An AFLP marker linked to the leaf rust resistance gene LrBi16 and test of allelism with Lr14a on chromosome arm 7BL

An AFLP marker linked to the leaf rust resistance gene LrBi16 and test of allelism with Lr14a on chromosome arm 7BL

Leaf rust (LR), caused by Puccinia triticina, is one of the most widespread diseases of common wheat (Triticum aestivum L.) worldwide. The LR resistance gene LrBi16 has been mapped on chromosome arm 7BL in Chinese wheat cultivar Bimai 16 and was closely linked to SSR loci Xcfa2257 and Xgwm344 with g...

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Main Authors: Peipei Zhang, Huixin Zho, Caixia Lan, Zaifeng Li, Daqun Liu
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2015-04-01
Series:Crop Journal
Subjects:
Amplified fragment length polymorphism
Genetic linkage
Molecular mapping
Puccinia triticina
Online Access:http://www.sciencedirect.com/science/article/pii/S2214514115000148
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spelling doaj-42111a78774349ac81fe2a6345afe1e42021-02-02T03:49:00ZengKeAi Communications Co., Ltd.Crop Journal2095-54212214-51412015-04-0132152156doi:10.1016/j.cj.2014.11.004An AFLP marker linked to the leaf rust resistance gene LrBi16 and test of allelism with Lr14a on chromosome arm 7BLPeipei Zhang 0Huixin Zho1Caixia Lan 2Zaifeng Li 3Daqun Liu 4Department of Plant Pathology, College of Plant Protection, Hebei Agricultural University, Baoding 071001, Hebei, ChinaDepartment of Plant Pathology, College of Plant Protection, Hebei Agricultural University, Baoding 071001, Hebei, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600 Mexico D.F., MexicoDepartment of Plant Pathology, College of Plant Protection, Hebei Agricultural University, Baoding 071001, Hebei, ChinaDepartment of Plant Pathology, College of Plant Protection, Hebei Agricultural University, Baoding 071001, Hebei, ChinaLeaf rust (LR), caused by Puccinia triticina, is one of the most widespread diseases of common wheat (Triticum aestivum L.) worldwide. The LR resistance gene LrBi16 has been mapped on chromosome arm 7BL in Chinese wheat cultivar Bimai 16 and was closely linked to SSR loci Xcfa2257 and Xgwm344 with genetic distances of 2.8 cM and 2.9 cM, respectively. In the present study, a total of 304 AFLP primer pairs were used to screen Bimai 16 and Thatcher and resistant and susceptible DNA bulks. The polymorphic AFLP marker P-ATT/M-CGC173 bp was used to genotype F2 and F3 populations to identify markers more closely linked to LrBi16. Marker P-ATT/M-CGC173 bp was tightly linked to LrBi16 with a genetic distance of 0.5 cM. As LrBi16 was mapped near the Lr14a locus, 809 F2 plants from the Bimai 16/RL6013 (Lr14a) cross were inoculated with the Pt pathotype FHNQ to test the allelism of Lr14a and LrBi16. All of the F2 plants were resistant to FHNQ (IT between; and 2), suggesting that Lr14a and LrBi16 are allelic.http://www.sciencedirect.com/science/article/pii/S2214514115000148Amplified fragment length polymorphismGenetic linkageMolecular mappingPuccinia triticina
collection DOAJ
language English
format Article
sources DOAJ
author Peipei Zhang
Huixin Zho
Caixia Lan
Zaifeng Li
Daqun Liu
spellingShingle Peipei Zhang
Huixin Zho
Caixia Lan
Zaifeng Li
Daqun Liu
An AFLP marker linked to the leaf rust resistance gene LrBi16 and test of allelism with Lr14a on chromosome arm 7BL
Crop Journal
Amplified fragment length polymorphism
Genetic linkage
Molecular mapping
Puccinia triticina
author_facet Peipei Zhang
Huixin Zho
Caixia Lan
Zaifeng Li
Daqun Liu
author_sort Peipei Zhang
title An AFLP marker linked to the leaf rust resistance gene LrBi16 and test of allelism with Lr14a on chromosome arm 7BL
title_short An AFLP marker linked to the leaf rust resistance gene LrBi16 and test of allelism with Lr14a on chromosome arm 7BL
title_full An AFLP marker linked to the leaf rust resistance gene LrBi16 and test of allelism with Lr14a on chromosome arm 7BL
title_fullStr An AFLP marker linked to the leaf rust resistance gene LrBi16 and test of allelism with Lr14a on chromosome arm 7BL
title_full_unstemmed An AFLP marker linked to the leaf rust resistance gene LrBi16 and test of allelism with Lr14a on chromosome arm 7BL
title_sort aflp marker linked to the leaf rust resistance gene lrbi16 and test of allelism with lr14a on chromosome arm 7bl
publisher KeAi Communications Co., Ltd.
series Crop Journal
issn 2095-5421
2214-5141
publishDate 2015-04-01
description Leaf rust (LR), caused by Puccinia triticina, is one of the most widespread diseases of common wheat (Triticum aestivum L.) worldwide. The LR resistance gene LrBi16 has been mapped on chromosome arm 7BL in Chinese wheat cultivar Bimai 16 and was closely linked to SSR loci Xcfa2257 and Xgwm344 with genetic distances of 2.8 cM and 2.9 cM, respectively. In the present study, a total of 304 AFLP primer pairs were used to screen Bimai 16 and Thatcher and resistant and susceptible DNA bulks. The polymorphic AFLP marker P-ATT/M-CGC173 bp was used to genotype F2 and F3 populations to identify markers more closely linked to LrBi16. Marker P-ATT/M-CGC173 bp was tightly linked to LrBi16 with a genetic distance of 0.5 cM. As LrBi16 was mapped near the Lr14a locus, 809 F2 plants from the Bimai 16/RL6013 (Lr14a) cross were inoculated with the Pt pathotype FHNQ to test the allelism of Lr14a and LrBi16. All of the F2 plants were resistant to FHNQ (IT between; and 2), suggesting that Lr14a and LrBi16 are allelic.
topic Amplified fragment length polymorphism
Genetic linkage
Molecular mapping
Puccinia triticina
url http://www.sciencedirect.com/science/article/pii/S2214514115000148
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