Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line

Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line

The ACTOne cannabinoid receptor 1 functional system is comprised of transfected HEK cells with the parental cyclic nucleotide gated channel (CNG) co-transfected with cannabinoid receptor 1 (CB1). The ACTOne CB1 cell line was evaluated for cAMP driven fluorescence by optimizing experimental condition...

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Main Authors: Chaela S. Presley, Ammaar H. Abidi, Bob M. Moore, II
Format: Article
Language:English
Published: Elsevier 2016-06-01
Series:Data in Brief
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340916301949
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spelling doaj-42114caf221e4c6ea720631b0746466f2020-11-25T01:25:55ZengElsevierData in Brief2352-34092016-06-01711181123Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell lineChaela S. Presley0Ammaar H. Abidi1Bob M. Moore, II2University of Tennessee Health Science Center, College of Pharmacy, United StatesUniversity of Tennessee Health Science Center, College of Pharmacy, United StatesUniversity of Tennessee Health Science Center, College of Pharmacy, United StatesThe ACTOne cannabinoid receptor 1 functional system is comprised of transfected HEK cells with the parental cyclic nucleotide gated channel (CNG) co-transfected with cannabinoid receptor 1 (CB1). The ACTOne CB1 cell line was evaluated for cAMP driven fluorescence by optimizing experimental conditions for sensitivity to forskolin and CP 55,940, reading time point, reliability of cell passage number, and pertussis inactivation of Gi/o. Keywords: ACTOne, Cannabinoids, CB1, Pharmacology, cAMPhttp://www.sciencedirect.com/science/article/pii/S2352340916301949
collection DOAJ
language English
format Article
sources DOAJ
author Chaela S. Presley
Ammaar H. Abidi
Bob M. Moore, II
spellingShingle Chaela S. Presley
Ammaar H. Abidi
Bob M. Moore, II
Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line
Data in Brief
author_facet Chaela S. Presley
Ammaar H. Abidi
Bob M. Moore, II
author_sort Chaela S. Presley
title Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line
title_short Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line
title_full Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line
title_fullStr Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line
title_full_unstemmed Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line
title_sort optimization of camp fluorescence dataset from actone cannabinoid receptor 1 cell line
publisher Elsevier
series Data in Brief
issn 2352-3409
publishDate 2016-06-01
description The ACTOne cannabinoid receptor 1 functional system is comprised of transfected HEK cells with the parental cyclic nucleotide gated channel (CNG) co-transfected with cannabinoid receptor 1 (CB1). The ACTOne CB1 cell line was evaluated for cAMP driven fluorescence by optimizing experimental conditions for sensitivity to forskolin and CP 55,940, reading time point, reliability of cell passage number, and pertussis inactivation of Gi/o. Keywords: ACTOne, Cannabinoids, CB1, Pharmacology, cAMP
url http://www.sciencedirect.com/science/article/pii/S2352340916301949
work_keys_str_mv AT chaelaspresley optimizationofcampfluorescencedatasetfromactonecannabinoidreceptor1cellline
AT ammaarhabidi optimizationofcampfluorescencedatasetfromactonecannabinoidreceptor1cellline
AT bobmmooreii optimizationofcampfluorescencedatasetfromactonecannabinoidreceptor1cellline
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