Gene Expression of Putative Pathogenicity-Related Genes in <i>Verticillium dahliae</i> in Response to Elicitation with Potato Extracts and during Infection Using Quantitative Real-Time PCR
Quantitative real-time PCR was used to monitor the expression of 15 <i>Verticillium dahliae</i>’s genes, putatively involved in pathogenicity, highly (HAV) and weakly aggressive (WAV) <i>V. dahliae</i> isolates after either (i) elicitation with potato leaf, stem, or root extr...
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doaj-421f552c475e49bda871b3d35437d91e2021-04-23T23:01:26ZengMDPI AGPathogens2076-08172021-04-011051051010.3390/pathogens10050510Gene Expression of Putative Pathogenicity-Related Genes in <i>Verticillium dahliae</i> in Response to Elicitation with Potato Extracts and during Infection Using Quantitative Real-Time PCRXiaohan Zhu0Arbia Arfaoui1Mohammad Sayari2Lorne R. Adam3Fouad Daayf4Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, CanadaDepartment of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, CanadaDepartment of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, CanadaDepartment of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, CanadaDepartment of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, CanadaQuantitative real-time PCR was used to monitor the expression of 15 <i>Verticillium dahliae</i>’s genes, putatively involved in pathogenicity, highly (HAV) and weakly aggressive (WAV) <i>V. dahliae</i> isolates after either (i) elicitation with potato leaf, stem, or root extracts, or (ii) inoculation of potato detached petioles. These genes, i.e., coding for Ras-GAP-like protein, serine/threonine protein kinase, Ubiquitin-conjugating enzyme variant-MMS2, NADH-ubiquinone oxidoreductase, Thioredoxin, Pyruvate dehydrogenase E1 VdPDHB, myo-inositol 2-dehydrogenase, and HAD-superfamily hydrolase, showed differential upregulation in the HAV versus WAV isolate in response to plant extracts or after inoculation of potato leaf petioles. This suggests their potential involvement in the observed differential aggressiveness between isolates. However, other genes like glucan endo-1,3-alpha-glucosidase and nuc-1 negative regulatory protein <i>VdPREG</i> showed higher activity in the WAV than in the HAV in response to potato extracts and/or during infection. This, in contrast, may suggest a role in their lower aggressiveness. These findings, along with future functional analysis of selected genes, will contribute to improving our understanding of <i>V. dahliae</i>’s pathogenesis. For example, expression of <i>VdPREG</i> negatively regulates phosphorus-acquisition enzymes, which may indicate a lower phosphorus acquisition activity in the WAV. Therefore, integrating the knowledge about the activity of both genes enhancing pathogenicity and those restraining it will provide a guild line for further functional characterization of the most critical genes, thus driving new ideas towards better Verticillium wilt management.https://www.mdpi.com/2076-0817/10/5/510<i>Verticillium dahliae</i>effectorsdefense inducershighly aggressivegene expression |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaohan Zhu Arbia Arfaoui Mohammad Sayari Lorne R. Adam Fouad Daayf |
spellingShingle |
Xiaohan Zhu Arbia Arfaoui Mohammad Sayari Lorne R. Adam Fouad Daayf Gene Expression of Putative Pathogenicity-Related Genes in <i>Verticillium dahliae</i> in Response to Elicitation with Potato Extracts and during Infection Using Quantitative Real-Time PCR Pathogens <i>Verticillium dahliae</i> effectors defense inducers highly aggressive gene expression |
author_facet |
Xiaohan Zhu Arbia Arfaoui Mohammad Sayari Lorne R. Adam Fouad Daayf |
author_sort |
Xiaohan Zhu |
title |
Gene Expression of Putative Pathogenicity-Related Genes in <i>Verticillium dahliae</i> in Response to Elicitation with Potato Extracts and during Infection Using Quantitative Real-Time PCR |
title_short |
Gene Expression of Putative Pathogenicity-Related Genes in <i>Verticillium dahliae</i> in Response to Elicitation with Potato Extracts and during Infection Using Quantitative Real-Time PCR |
title_full |
Gene Expression of Putative Pathogenicity-Related Genes in <i>Verticillium dahliae</i> in Response to Elicitation with Potato Extracts and during Infection Using Quantitative Real-Time PCR |
title_fullStr |
Gene Expression of Putative Pathogenicity-Related Genes in <i>Verticillium dahliae</i> in Response to Elicitation with Potato Extracts and during Infection Using Quantitative Real-Time PCR |
title_full_unstemmed |
Gene Expression of Putative Pathogenicity-Related Genes in <i>Verticillium dahliae</i> in Response to Elicitation with Potato Extracts and during Infection Using Quantitative Real-Time PCR |
title_sort |
gene expression of putative pathogenicity-related genes in <i>verticillium dahliae</i> in response to elicitation with potato extracts and during infection using quantitative real-time pcr |
publisher |
MDPI AG |
series |
Pathogens |
issn |
2076-0817 |
publishDate |
2021-04-01 |
description |
Quantitative real-time PCR was used to monitor the expression of 15 <i>Verticillium dahliae</i>’s genes, putatively involved in pathogenicity, highly (HAV) and weakly aggressive (WAV) <i>V. dahliae</i> isolates after either (i) elicitation with potato leaf, stem, or root extracts, or (ii) inoculation of potato detached petioles. These genes, i.e., coding for Ras-GAP-like protein, serine/threonine protein kinase, Ubiquitin-conjugating enzyme variant-MMS2, NADH-ubiquinone oxidoreductase, Thioredoxin, Pyruvate dehydrogenase E1 VdPDHB, myo-inositol 2-dehydrogenase, and HAD-superfamily hydrolase, showed differential upregulation in the HAV versus WAV isolate in response to plant extracts or after inoculation of potato leaf petioles. This suggests their potential involvement in the observed differential aggressiveness between isolates. However, other genes like glucan endo-1,3-alpha-glucosidase and nuc-1 negative regulatory protein <i>VdPREG</i> showed higher activity in the WAV than in the HAV in response to potato extracts and/or during infection. This, in contrast, may suggest a role in their lower aggressiveness. These findings, along with future functional analysis of selected genes, will contribute to improving our understanding of <i>V. dahliae</i>’s pathogenesis. For example, expression of <i>VdPREG</i> negatively regulates phosphorus-acquisition enzymes, which may indicate a lower phosphorus acquisition activity in the WAV. Therefore, integrating the knowledge about the activity of both genes enhancing pathogenicity and those restraining it will provide a guild line for further functional characterization of the most critical genes, thus driving new ideas towards better Verticillium wilt management. |
topic |
<i>Verticillium dahliae</i> effectors defense inducers highly aggressive gene expression |
url |
https://www.mdpi.com/2076-0817/10/5/510 |
work_keys_str_mv |
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