The Potential Health Benefits of Polyphenol-Rich Extracts from Cichorium intybus L. Studied on Caco-2 Cells Model
Phytochemicals can exert their bioactivity without reaching the systemic circulation; scarcely absorbed antioxidants might reach the large bowel contributing to protection from oxidative damage-induced gastrointestinal diseases. In the present work, we aimed to study the relationship between potenti...
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2016-01-01
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Series: | Oxidative Medicine and Cellular Longevity |
Online Access: | http://dx.doi.org/10.1155/2016/1594616 |
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doaj-4251956d4ba34261bb209ed3a1e73e512020-11-24T23:54:19ZengHindawi LimitedOxidative Medicine and Cellular Longevity1942-09001942-09942016-01-01201610.1155/2016/15946161594616The Potential Health Benefits of Polyphenol-Rich Extracts from Cichorium intybus L. Studied on Caco-2 Cells ModelElena Azzini0Giuseppe Maiani1Ivana Garaguso2Angela Polito3Maria S. Foddai4Eugenia Venneria5Alessandra Durazzo6Federica Intorre7Lara Palomba8Maria L. Rauseo9Ginevra Lombardi-Boccia10Fabio Nobili11Council for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, ItalyCouncil for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, ItalyCouncil for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, ItalyCouncil for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, ItalyCouncil for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, ItalyCouncil for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, ItalyCouncil for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, ItalyCouncil for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, ItalyCouncil for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, ItalyCouncil for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, ItalyCouncil for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, ItalyCouncil for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, ItalyPhytochemicals can exert their bioactivity without reaching the systemic circulation; scarcely absorbed antioxidants might reach the large bowel contributing to protection from oxidative damage-induced gastrointestinal diseases. In the present work, we aimed to study the relationship between potential activity of polyphenol-rich extracts from Cichorium intybus L. and changes in morphological characteristics on Caco-2 cells. Phytochemicals content (carotenoids and flavonoids) and total antioxidant activity of Red Chicory of Treviso and Variegated Chicory of Castelfranco were evaluated. The bioactivity of polyphenol-rich extracts from chicories was studied in in vitro Caco-2 cell monolayers model. Morphological characteristics changes to test the antioxidant and/or prooxidant effect were verified by histological analysis and observed by Electronic Scansion Microscopy (SEM). On Caco-2 cell model, the polyphenols fractions from chicories have indicated a moderate antioxidant behavior until 17 μM concentration, while 70 μM and 34 μM exert cytotoxic effects for Treviso’s and Castelfranco’s Chicory, respectively, highlighted by TEER decreasing, increased permeability, and alteration of epithelium. Our findings support the beneficial effects of these products in counteracting the oxidative stress and cellular damage, induced in vitro on Caco-2 cell model, through interaction with the mucopolysaccharide complexes in the glycocalyx, maintaining in vivo a healthy and effective intestinal barrier.http://dx.doi.org/10.1155/2016/1594616 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elena Azzini Giuseppe Maiani Ivana Garaguso Angela Polito Maria S. Foddai Eugenia Venneria Alessandra Durazzo Federica Intorre Lara Palomba Maria L. Rauseo Ginevra Lombardi-Boccia Fabio Nobili |
spellingShingle |
Elena Azzini Giuseppe Maiani Ivana Garaguso Angela Polito Maria S. Foddai Eugenia Venneria Alessandra Durazzo Federica Intorre Lara Palomba Maria L. Rauseo Ginevra Lombardi-Boccia Fabio Nobili The Potential Health Benefits of Polyphenol-Rich Extracts from Cichorium intybus L. Studied on Caco-2 Cells Model Oxidative Medicine and Cellular Longevity |
author_facet |
Elena Azzini Giuseppe Maiani Ivana Garaguso Angela Polito Maria S. Foddai Eugenia Venneria Alessandra Durazzo Federica Intorre Lara Palomba Maria L. Rauseo Ginevra Lombardi-Boccia Fabio Nobili |
author_sort |
Elena Azzini |
title |
The Potential Health Benefits of Polyphenol-Rich Extracts from Cichorium intybus L. Studied on Caco-2 Cells Model |
title_short |
The Potential Health Benefits of Polyphenol-Rich Extracts from Cichorium intybus L. Studied on Caco-2 Cells Model |
title_full |
The Potential Health Benefits of Polyphenol-Rich Extracts from Cichorium intybus L. Studied on Caco-2 Cells Model |
title_fullStr |
The Potential Health Benefits of Polyphenol-Rich Extracts from Cichorium intybus L. Studied on Caco-2 Cells Model |
title_full_unstemmed |
The Potential Health Benefits of Polyphenol-Rich Extracts from Cichorium intybus L. Studied on Caco-2 Cells Model |
title_sort |
potential health benefits of polyphenol-rich extracts from cichorium intybus l. studied on caco-2 cells model |
publisher |
Hindawi Limited |
series |
Oxidative Medicine and Cellular Longevity |
issn |
1942-0900 1942-0994 |
publishDate |
2016-01-01 |
description |
Phytochemicals can exert their bioactivity without reaching the systemic circulation; scarcely absorbed antioxidants might reach the large bowel contributing to protection from oxidative damage-induced gastrointestinal diseases. In the present work, we aimed to study the relationship between potential activity of polyphenol-rich extracts from Cichorium intybus L. and changes in morphological characteristics on Caco-2 cells. Phytochemicals content (carotenoids and flavonoids) and total antioxidant activity of Red Chicory of Treviso and Variegated Chicory of Castelfranco were evaluated. The bioactivity of polyphenol-rich extracts from chicories was studied in in vitro Caco-2 cell monolayers model. Morphological characteristics changes to test the antioxidant and/or prooxidant effect were verified by histological analysis and observed by Electronic Scansion Microscopy (SEM). On Caco-2 cell model, the polyphenols fractions from chicories have indicated a moderate antioxidant behavior until 17 μM concentration, while 70 μM and 34 μM exert cytotoxic effects for Treviso’s and Castelfranco’s Chicory, respectively, highlighted by TEER decreasing, increased permeability, and alteration of epithelium. Our findings support the beneficial effects of these products in counteracting the oxidative stress and cellular damage, induced in vitro on Caco-2 cell model, through interaction with the mucopolysaccharide complexes in the glycocalyx, maintaining in vivo a healthy and effective intestinal barrier. |
url |
http://dx.doi.org/10.1155/2016/1594616 |
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