Exosomes Secreted from Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Accelerate Skin Cell Proliferation
Induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) serve as a unique source for cell therapy. We investigated whether exosomes from iMSCs promote the proliferation of human keratinocytes (HaCaT) and human dermal fibroblasts (HDFs). iPSCs were established from human Wharton’s...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2018-10-01
|
Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | http://www.mdpi.com/1422-0067/19/10/3119 |
id |
doaj-4267f51ef1cd4534b5b34048d5dab4c2 |
---|---|
record_format |
Article |
spelling |
doaj-4267f51ef1cd4534b5b34048d5dab4c22020-11-25T00:23:41ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-10-011910311910.3390/ijms19103119ijms19103119Exosomes Secreted from Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Accelerate Skin Cell ProliferationSoo Kim0Seul Ki Lee1Hyunjung Kim2Tae Min Kim3Stem Cell Center, Asan Institute for Life Science, Asan Medical Center, Seoul 05505, KoreaStem Cell Center, Asan Institute for Life Science, Asan Medical Center, Seoul 05505, KoreaStem Cell Center, Asan Institute for Life Science, Asan Medical Center, Seoul 05505, KoreaGraduate School of International Agricultural Technology and Institute of Green-Bio Science and Technology, Pyeongchang Daero 1447, Seoul National University, Pyeongchang, Gangwon-do 25354, KoreaInduced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) serve as a unique source for cell therapy. We investigated whether exosomes from iMSCs promote the proliferation of human keratinocytes (HaCaT) and human dermal fibroblasts (HDFs). iPSCs were established from human Wharton’s jelly MSCs and were allowed to differentiate into iMSCs. Exosomes were collected from the culture supernatant of MSCs (MSC-exo) and iMSCs (iMSC-exo), and their characteristics were investigated. Both exosome types possessed basic characteristics of exosomes and were taken up by skin cells in vitro and in vivo. A significant increase in HaCaT proliferation was observed with iMSC-exo, although both exosomes increased the viability and cell cycle progression in HaCaT and HDFs. No significant difference was observed in the closure of wound scratch and the expression of reparative genes between cells treated with the two exosome types. Both exosomes enhanced the secretion of collagen in HaCaT and HDFs; however, an increase in fibronectin level was observed only in HaCaT, and this effect was better with iMSC-exo treatment. Only iMSC-exo increased the phosphorylation of extracellular signal-regulated kinase (ERK)-1/2. Our results indicate that iMSC-exo promote the proliferation of skin cells by stimulating ERK1/2 and highlight the application of iMSCs for producing exosomes.http://www.mdpi.com/1422-0067/19/10/3119iPSCsMSCsexosomewound healing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Soo Kim Seul Ki Lee Hyunjung Kim Tae Min Kim |
spellingShingle |
Soo Kim Seul Ki Lee Hyunjung Kim Tae Min Kim Exosomes Secreted from Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Accelerate Skin Cell Proliferation International Journal of Molecular Sciences iPSCs MSCs exosome wound healing |
author_facet |
Soo Kim Seul Ki Lee Hyunjung Kim Tae Min Kim |
author_sort |
Soo Kim |
title |
Exosomes Secreted from Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Accelerate Skin Cell Proliferation |
title_short |
Exosomes Secreted from Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Accelerate Skin Cell Proliferation |
title_full |
Exosomes Secreted from Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Accelerate Skin Cell Proliferation |
title_fullStr |
Exosomes Secreted from Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Accelerate Skin Cell Proliferation |
title_full_unstemmed |
Exosomes Secreted from Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Accelerate Skin Cell Proliferation |
title_sort |
exosomes secreted from induced pluripotent stem cell-derived mesenchymal stem cells accelerate skin cell proliferation |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2018-10-01 |
description |
Induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) serve as a unique source for cell therapy. We investigated whether exosomes from iMSCs promote the proliferation of human keratinocytes (HaCaT) and human dermal fibroblasts (HDFs). iPSCs were established from human Wharton’s jelly MSCs and were allowed to differentiate into iMSCs. Exosomes were collected from the culture supernatant of MSCs (MSC-exo) and iMSCs (iMSC-exo), and their characteristics were investigated. Both exosome types possessed basic characteristics of exosomes and were taken up by skin cells in vitro and in vivo. A significant increase in HaCaT proliferation was observed with iMSC-exo, although both exosomes increased the viability and cell cycle progression in HaCaT and HDFs. No significant difference was observed in the closure of wound scratch and the expression of reparative genes between cells treated with the two exosome types. Both exosomes enhanced the secretion of collagen in HaCaT and HDFs; however, an increase in fibronectin level was observed only in HaCaT, and this effect was better with iMSC-exo treatment. Only iMSC-exo increased the phosphorylation of extracellular signal-regulated kinase (ERK)-1/2. Our results indicate that iMSC-exo promote the proliferation of skin cells by stimulating ERK1/2 and highlight the application of iMSCs for producing exosomes. |
topic |
iPSCs MSCs exosome wound healing |
url |
http://www.mdpi.com/1422-0067/19/10/3119 |
work_keys_str_mv |
AT sookim exosomessecretedfrominducedpluripotentstemcellderivedmesenchymalstemcellsaccelerateskincellproliferation AT seulkilee exosomessecretedfrominducedpluripotentstemcellderivedmesenchymalstemcellsaccelerateskincellproliferation AT hyunjungkim exosomessecretedfrominducedpluripotentstemcellderivedmesenchymalstemcellsaccelerateskincellproliferation AT taeminkim exosomessecretedfrominducedpluripotentstemcellderivedmesenchymalstemcellsaccelerateskincellproliferation |
_version_ |
1725355600679796736 |