Metatranscriptomic and metagenomic description of the bacterial nitrogen metabolism in waste water wet oxidation effluents
Anaerobic digestion is a common method for reducing the amount of sludge solids in used waters and enabling biogas production. The wet oxidation process (WOX) improves anaerobic digestion by converting carbon into methane through oxidation of organic compounds. WOX produces effluents rich in ammonia...
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doaj-426cb3ef17dc49af9cee718560c087582020-11-25T02:14:04ZengElsevierHeliyon2405-84402017-10-01310e00427Metatranscriptomic and metagenomic description of the bacterial nitrogen metabolism in waste water wet oxidation effluentsJulien Crovadore0Vice Soljan1Gautier Calmin2Romain Chablais3Bastien Cochard4François Lefort5Plants and pathogens group, Institute Land Nature and Environment, Hepia, HES-SO University of Applied Sciences and Arts Western Switzerland, 150 route de Presinge, 1254 Jussy, SwitzerlandPuratis Sàrl, EPFL Innovation Park, Building C, 1015 Lausanne, SwitzerlandFaculty of Engineering and Architecture, HES-SO University of Applied Sciences and Arts Western Switzerland, Rue de la Jeunesse 1, 2800 Delémont, SwitzerlandPlants and pathogens group, Institute Land Nature and Environment, Hepia, HES-SO University of Applied Sciences and Arts Western Switzerland, 150 route de Presinge, 1254 Jussy, SwitzerlandPlants and pathogens group, Institute Land Nature and Environment, Hepia, HES-SO University of Applied Sciences and Arts Western Switzerland, 150 route de Presinge, 1254 Jussy, SwitzerlandPlants and pathogens group, Institute Land Nature and Environment, Hepia, HES-SO University of Applied Sciences and Arts Western Switzerland, 150 route de Presinge, 1254 Jussy, Switzerland; Corresponding author.Anaerobic digestion is a common method for reducing the amount of sludge solids in used waters and enabling biogas production. The wet oxidation process (WOX) improves anaerobic digestion by converting carbon into methane through oxidation of organic compounds. WOX produces effluents rich in ammonia, which must be removed to maintain the activity of methanogens. Ammonia removal from WOX could be biologically operated by aerobic granules. To this end, granulation experiments were conducted in 2 bioreactors containing an activated sludge (AS). For the first time, the dynamics of the microbial community structure and the expression levels of 7 enzymes of the nitrogen metabolism in such active microbial communities were followed in regard to time by metagenomics and metatranscriptomics. It was shown that bacterial communities adapt to the wet oxidation effluent by increasing the expression level of the nitrogen metabolism, suggesting that these biological activities could be a less costly alternative for the elimination of ammonia, resulting in a reduction of the use of chemicals and energy consumption in sewage plants. This study reached a strong sequencing depth (from 4.4 to 7.6 Gb) and enlightened a yet unknown diversity of the microorganisms involved in the nitrogen pathway. Moreover, this approach revealed the abundance and expression levels of specialised enzymes involved in nitrification, denitrification, ammonification, dissimilatory nitrate reduction to ammonium (DNRA) and nitrogen fixation processes in AS.http://www.sciencedirect.com/science/article/pii/S240584401731280XApplied sciencesBiological sciencesEnvironmental scienceGeneticsMicrobiology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Julien Crovadore Vice Soljan Gautier Calmin Romain Chablais Bastien Cochard François Lefort |
spellingShingle |
Julien Crovadore Vice Soljan Gautier Calmin Romain Chablais Bastien Cochard François Lefort Metatranscriptomic and metagenomic description of the bacterial nitrogen metabolism in waste water wet oxidation effluents Heliyon Applied sciences Biological sciences Environmental science Genetics Microbiology |
author_facet |
Julien Crovadore Vice Soljan Gautier Calmin Romain Chablais Bastien Cochard François Lefort |
author_sort |
Julien Crovadore |
title |
Metatranscriptomic and metagenomic description of the bacterial nitrogen metabolism in waste water wet oxidation effluents |
title_short |
Metatranscriptomic and metagenomic description of the bacterial nitrogen metabolism in waste water wet oxidation effluents |
title_full |
Metatranscriptomic and metagenomic description of the bacterial nitrogen metabolism in waste water wet oxidation effluents |
title_fullStr |
Metatranscriptomic and metagenomic description of the bacterial nitrogen metabolism in waste water wet oxidation effluents |
title_full_unstemmed |
Metatranscriptomic and metagenomic description of the bacterial nitrogen metabolism in waste water wet oxidation effluents |
title_sort |
metatranscriptomic and metagenomic description of the bacterial nitrogen metabolism in waste water wet oxidation effluents |
publisher |
Elsevier |
series |
Heliyon |
issn |
2405-8440 |
publishDate |
2017-10-01 |
description |
Anaerobic digestion is a common method for reducing the amount of sludge solids in used waters and enabling biogas production. The wet oxidation process (WOX) improves anaerobic digestion by converting carbon into methane through oxidation of organic compounds. WOX produces effluents rich in ammonia, which must be removed to maintain the activity of methanogens. Ammonia removal from WOX could be biologically operated by aerobic granules. To this end, granulation experiments were conducted in 2 bioreactors containing an activated sludge (AS). For the first time, the dynamics of the microbial community structure and the expression levels of 7 enzymes of the nitrogen metabolism in such active microbial communities were followed in regard to time by metagenomics and metatranscriptomics. It was shown that bacterial communities adapt to the wet oxidation effluent by increasing the expression level of the nitrogen metabolism, suggesting that these biological activities could be a less costly alternative for the elimination of ammonia, resulting in a reduction of the use of chemicals and energy consumption in sewage plants. This study reached a strong sequencing depth (from 4.4 to 7.6 Gb) and enlightened a yet unknown diversity of the microorganisms involved in the nitrogen pathway. Moreover, this approach revealed the abundance and expression levels of specialised enzymes involved in nitrification, denitrification, ammonification, dissimilatory nitrate reduction to ammonium (DNRA) and nitrogen fixation processes in AS. |
topic |
Applied sciences Biological sciences Environmental science Genetics Microbiology |
url |
http://www.sciencedirect.com/science/article/pii/S240584401731280X |
work_keys_str_mv |
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