Macrophage Biocompatibility of CoCr Wear Particles Produced under Polarization in Hyaluronic Acid Aqueous Solution
Macrophages are the main cells involved in inflammatory processes and in the primary response to debris derived from wear of implanted CoCr alloys. The biocompatibility of wear particles from a high carbon CoCr alloy produced under polarization in hyaluronic acid (HA) aqueous solution was evaluated...
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doaj-4271920a564a475ab143b12a9a2df2de2020-11-25T00:29:55ZengMDPI AGMaterials1996-19442018-05-0111575610.3390/ma11050756ma11050756Macrophage Biocompatibility of CoCr Wear Particles Produced under Polarization in Hyaluronic Acid Aqueous SolutionBlanca Teresa Perez-Maceda0María Encarnación López-Fernández1Iván Díaz2Aaron Kavanaugh3Fabrizio Billi4María Lorenza Escudero5María Cristina García-Alonso6Rosa María Lozano7Cell-Biomaterial Recognition Lab., Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040 Madrid, SpainCell-Biomaterial Recognition Lab., Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040 Madrid, SpainDepartment of Surface Engineering, Corrosion and Durability, Centro Nacional de Investigaciones Metalúrgicas (CENIM-CSIC), Avda. Gregorio del Amo 8, 28040 Madrid, SpainDepartment of Orthopaedic Surgery, David Geffen School of Medicine, University of California Los Angeles, Orthopaedic Hospital Research Center, 615 Charles E. Young Dr. South, Room 450A, Los Angeles, CA 90095 USADepartment of Orthopaedic Surgery, David Geffen School of Medicine, University of California Los Angeles, Orthopaedic Hospital Research Center, 615 Charles E. Young Dr. South, Room 450A, Los Angeles, CA 90095 USADepartment of Surface Engineering, Corrosion and Durability, Centro Nacional de Investigaciones Metalúrgicas (CENIM-CSIC), Avda. Gregorio del Amo 8, 28040 Madrid, SpainDepartment of Surface Engineering, Corrosion and Durability, Centro Nacional de Investigaciones Metalúrgicas (CENIM-CSIC), Avda. Gregorio del Amo 8, 28040 Madrid, SpainCell-Biomaterial Recognition Lab., Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040 Madrid, SpainMacrophages are the main cells involved in inflammatory processes and in the primary response to debris derived from wear of implanted CoCr alloys. The biocompatibility of wear particles from a high carbon CoCr alloy produced under polarization in hyaluronic acid (HA) aqueous solution was evaluated in J774A.1 mouse macrophages cultures. Polarization was applied to mimic the electrical interactions observed in living tissues. Wear tests were performed in a pin-on-disk tribometer integrating an electrochemical cell in phosphate buffer solution (PBS) and in PBS supplemented with 3 g/L HA, an average concentration that is generally found in synovial fluid, used as lubricant solution. Wear particles produced in 3 g/L HA solution showed a higher biocompatibility in J774A.1 macrophages in comparison to those elicited by particles obtained in PBS. A considerable enhancement in macrophages biocompatibility in the presence of 3 g/L of HA was further observed by the application of polarization at potentials having current densities typical of injured tissues suggesting that polarization produces an effect on the surface of the metallic material that leads to the production of wear particles that seem to be macrophage-biocompatible and less cytotoxic. The results showed the convenience of considering the influence of the electric interactions in the chemical composition of debris detached from metallic surfaces under wear corrosion to get a better understanding of the biological effects caused by the wear products.http://www.mdpi.com/1996-1944/11/5/756polarizationCoCr alloywear particleshyaluronic acidmacrophages biocompatibility |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Blanca Teresa Perez-Maceda María Encarnación López-Fernández Iván Díaz Aaron Kavanaugh Fabrizio Billi María Lorenza Escudero María Cristina García-Alonso Rosa María Lozano |
spellingShingle |
Blanca Teresa Perez-Maceda María Encarnación López-Fernández Iván Díaz Aaron Kavanaugh Fabrizio Billi María Lorenza Escudero María Cristina García-Alonso Rosa María Lozano Macrophage Biocompatibility of CoCr Wear Particles Produced under Polarization in Hyaluronic Acid Aqueous Solution Materials polarization CoCr alloy wear particles hyaluronic acid macrophages biocompatibility |
author_facet |
Blanca Teresa Perez-Maceda María Encarnación López-Fernández Iván Díaz Aaron Kavanaugh Fabrizio Billi María Lorenza Escudero María Cristina García-Alonso Rosa María Lozano |
author_sort |
Blanca Teresa Perez-Maceda |
title |
Macrophage Biocompatibility of CoCr Wear Particles Produced under Polarization in Hyaluronic Acid Aqueous Solution |
title_short |
Macrophage Biocompatibility of CoCr Wear Particles Produced under Polarization in Hyaluronic Acid Aqueous Solution |
title_full |
Macrophage Biocompatibility of CoCr Wear Particles Produced under Polarization in Hyaluronic Acid Aqueous Solution |
title_fullStr |
Macrophage Biocompatibility of CoCr Wear Particles Produced under Polarization in Hyaluronic Acid Aqueous Solution |
title_full_unstemmed |
Macrophage Biocompatibility of CoCr Wear Particles Produced under Polarization in Hyaluronic Acid Aqueous Solution |
title_sort |
macrophage biocompatibility of cocr wear particles produced under polarization in hyaluronic acid aqueous solution |
publisher |
MDPI AG |
series |
Materials |
issn |
1996-1944 |
publishDate |
2018-05-01 |
description |
Macrophages are the main cells involved in inflammatory processes and in the primary response to debris derived from wear of implanted CoCr alloys. The biocompatibility of wear particles from a high carbon CoCr alloy produced under polarization in hyaluronic acid (HA) aqueous solution was evaluated in J774A.1 mouse macrophages cultures. Polarization was applied to mimic the electrical interactions observed in living tissues. Wear tests were performed in a pin-on-disk tribometer integrating an electrochemical cell in phosphate buffer solution (PBS) and in PBS supplemented with 3 g/L HA, an average concentration that is generally found in synovial fluid, used as lubricant solution. Wear particles produced in 3 g/L HA solution showed a higher biocompatibility in J774A.1 macrophages in comparison to those elicited by particles obtained in PBS. A considerable enhancement in macrophages biocompatibility in the presence of 3 g/L of HA was further observed by the application of polarization at potentials having current densities typical of injured tissues suggesting that polarization produces an effect on the surface of the metallic material that leads to the production of wear particles that seem to be macrophage-biocompatible and less cytotoxic. The results showed the convenience of considering the influence of the electric interactions in the chemical composition of debris detached from metallic surfaces under wear corrosion to get a better understanding of the biological effects caused by the wear products. |
topic |
polarization CoCr alloy wear particles hyaluronic acid macrophages biocompatibility |
url |
http://www.mdpi.com/1996-1944/11/5/756 |
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