Subanesthetic Isoflurane Reduces Zymosan-Induced Inflammation in Murine Kupffer Cells by Inhibiting ROS-Activated p38 MAPK/NF-κB Signaling
Volatile anesthetic isoflurane (ISO) has immunomodulatory effects. The fungal component zymosan (ZY) induces inflammation through toll-like receptor 2 or dectin-1 signaling. We investigated the molecular actions of subanesthetic (0.7%) ISO against ZY-induced inflammatory activation in murine Kupffer...
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Format: | Article |
Language: | English |
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Hindawi Limited
2014-01-01
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Series: | Oxidative Medicine and Cellular Longevity |
Online Access: | http://dx.doi.org/10.1155/2014/851692 |
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doaj-427479ce9ee2432d931cbde1a5a5d4a7 |
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Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hui Wang Lei Wang Nan-lin Li Jun-tang Li Feng Yu Ya-li Zhao Ling Wang Jun Yi Ling Wang Jie-fang Bian Jiang-hao Chen Shi-fang Yuan Ting Wang Yong-gang Lv Ning-ning Liu Xiao-shan Zhu Rui Ling Jun Yun |
spellingShingle |
Hui Wang Lei Wang Nan-lin Li Jun-tang Li Feng Yu Ya-li Zhao Ling Wang Jun Yi Ling Wang Jie-fang Bian Jiang-hao Chen Shi-fang Yuan Ting Wang Yong-gang Lv Ning-ning Liu Xiao-shan Zhu Rui Ling Jun Yun Subanesthetic Isoflurane Reduces Zymosan-Induced Inflammation in Murine Kupffer Cells by Inhibiting ROS-Activated p38 MAPK/NF-κB Signaling Oxidative Medicine and Cellular Longevity |
author_facet |
Hui Wang Lei Wang Nan-lin Li Jun-tang Li Feng Yu Ya-li Zhao Ling Wang Jun Yi Ling Wang Jie-fang Bian Jiang-hao Chen Shi-fang Yuan Ting Wang Yong-gang Lv Ning-ning Liu Xiao-shan Zhu Rui Ling Jun Yun |
author_sort |
Hui Wang |
title |
Subanesthetic Isoflurane Reduces Zymosan-Induced Inflammation in Murine Kupffer Cells by Inhibiting ROS-Activated p38 MAPK/NF-κB Signaling |
title_short |
Subanesthetic Isoflurane Reduces Zymosan-Induced Inflammation in Murine Kupffer Cells by Inhibiting ROS-Activated p38 MAPK/NF-κB Signaling |
title_full |
Subanesthetic Isoflurane Reduces Zymosan-Induced Inflammation in Murine Kupffer Cells by Inhibiting ROS-Activated p38 MAPK/NF-κB Signaling |
title_fullStr |
Subanesthetic Isoflurane Reduces Zymosan-Induced Inflammation in Murine Kupffer Cells by Inhibiting ROS-Activated p38 MAPK/NF-κB Signaling |
title_full_unstemmed |
Subanesthetic Isoflurane Reduces Zymosan-Induced Inflammation in Murine Kupffer Cells by Inhibiting ROS-Activated p38 MAPK/NF-κB Signaling |
title_sort |
subanesthetic isoflurane reduces zymosan-induced inflammation in murine kupffer cells by inhibiting ros-activated p38 mapk/nf-κb signaling |
publisher |
Hindawi Limited |
series |
Oxidative Medicine and Cellular Longevity |
issn |
1942-0900 1942-0994 |
publishDate |
2014-01-01 |
description |
Volatile anesthetic isoflurane (ISO) has immunomodulatory effects. The fungal component zymosan (ZY) induces inflammation through toll-like receptor 2 or dectin-1 signaling. We investigated the molecular actions of subanesthetic (0.7%) ISO against ZY-induced inflammatory activation in murine Kupffer cells (KCs), which are known as the resident macrophages within the liver. We observed that ISO reduced ZY-induced cyclooxygenase 2 upregulation and prostaglandin E2 release, as determined by western blot and radioimmunoassay, respectively. ISO also reduced the production of tumor necrosis factor-α, interleukin-1β, IL-6, high-mobility group box-1, macrophage inflammatory protein-1α, macrophage inflammatory protein-2, and monocyte chemoattractant protein-1 as assessed by enzyme-linked immunosorbent assays. ISO blocked the ZY-induced nuclear translocation and DNA-binding activity of nuclear factor- (NF)-κB p65. Moreover, ISO attenuated ZY-induced p38 mitogen-activated protein kinase (MAPK) activation partly by scavenging reactive oxygen species (ROS); the interregulation that ROS activated p38 MAPK followed by NF-κB activation was crucial for the ZY-induced inflammatory responses in KCs. An in vivo study by peritoneal injection of ZY into BALB/C mice confirmed the anti-inflammatory properties of 0.7% ISO against ZY in KCs. These results suggest that ISO ameliorates ZY-induced inflammatory responses in murine KCs by inhibiting the interconnected ROS/p38 MAPK/NF-κB signaling pathways. |
url |
http://dx.doi.org/10.1155/2014/851692 |
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doaj-427479ce9ee2432d931cbde1a5a5d4a72020-11-24T22:15:20ZengHindawi LimitedOxidative Medicine and Cellular Longevity1942-09001942-09942014-01-01201410.1155/2014/851692851692Subanesthetic Isoflurane Reduces Zymosan-Induced Inflammation in Murine Kupffer Cells by Inhibiting ROS-Activated p38 MAPK/NF-κB SignalingHui Wang0Lei Wang1Nan-lin Li2Jun-tang Li3Feng Yu4Ya-li Zhao5Ling Wang6Jun Yi7Ling Wang8Jie-fang Bian9Jiang-hao Chen10Shi-fang Yuan11Ting Wang12Yong-gang Lv13Ning-ning Liu14Xiao-shan Zhu15Rui Ling16Jun Yun17Department of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, No. 15 Changle West Road, Xi’an, Shaanxi 710032, ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, No. 15 Changle West Road, Xi’an, Shaanxi 710032, ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, No. 15 Changle West Road, Xi’an, Shaanxi 710032, ChinaInstitute of Anal-Colorectal Surgery, No. 150 Central Hospital of PLA, Luoyang, Henan 451000, ChinaCentre of Material and Drug Supply, No. 150 Central Hospital of PLA, Luoyang, Henan 451000, ChinaCentre of Material and Drug Supply, No. 150 Central Hospital of PLA, Luoyang, Henan 451000, ChinaDepartment of Anesthesiology, No. 150 Central Hospital of PLA, Luoyang, Henan 451000, ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, No. 15 Changle West Road, Xi’an, Shaanxi 710032, ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, No. 15 Changle West Road, Xi’an, Shaanxi 710032, ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, No. 15 Changle West Road, Xi’an, Shaanxi 710032, ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, No. 15 Changle West Road, Xi’an, Shaanxi 710032, ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, No. 15 Changle West Road, Xi’an, Shaanxi 710032, ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, No. 15 Changle West Road, Xi’an, Shaanxi 710032, ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, No. 15 Changle West Road, Xi’an, Shaanxi 710032, ChinaInstitute of Anal-Colorectal Surgery, No. 150 Central Hospital of PLA, Luoyang, Henan 451000, ChinaInstitute of Anal-Colorectal Surgery, No. 150 Central Hospital of PLA, Luoyang, Henan 451000, ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, No. 15 Changle West Road, Xi’an, Shaanxi 710032, ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, No. 15 Changle West Road, Xi’an, Shaanxi 710032, ChinaVolatile anesthetic isoflurane (ISO) has immunomodulatory effects. The fungal component zymosan (ZY) induces inflammation through toll-like receptor 2 or dectin-1 signaling. We investigated the molecular actions of subanesthetic (0.7%) ISO against ZY-induced inflammatory activation in murine Kupffer cells (KCs), which are known as the resident macrophages within the liver. We observed that ISO reduced ZY-induced cyclooxygenase 2 upregulation and prostaglandin E2 release, as determined by western blot and radioimmunoassay, respectively. ISO also reduced the production of tumor necrosis factor-α, interleukin-1β, IL-6, high-mobility group box-1, macrophage inflammatory protein-1α, macrophage inflammatory protein-2, and monocyte chemoattractant protein-1 as assessed by enzyme-linked immunosorbent assays. ISO blocked the ZY-induced nuclear translocation and DNA-binding activity of nuclear factor- (NF)-κB p65. Moreover, ISO attenuated ZY-induced p38 mitogen-activated protein kinase (MAPK) activation partly by scavenging reactive oxygen species (ROS); the interregulation that ROS activated p38 MAPK followed by NF-κB activation was crucial for the ZY-induced inflammatory responses in KCs. An in vivo study by peritoneal injection of ZY into BALB/C mice confirmed the anti-inflammatory properties of 0.7% ISO against ZY in KCs. These results suggest that ISO ameliorates ZY-induced inflammatory responses in murine KCs by inhibiting the interconnected ROS/p38 MAPK/NF-κB signaling pathways.http://dx.doi.org/10.1155/2014/851692 |