Transcription strategies related to photosynthesis and nitrogen metabolism of wheat in response to nitrogen deficiency
Abstract Background Agricultural yield is closely associated with nitrogen application. Thus, reducing the application of nitrogen without affecting agricultural production remains a challenging task. To understand the metabolic, physiological, and morphological response of wheat (Triticum aestivum)...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2020-10-01
|
Series: | BMC Plant Biology |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12870-020-02662-3 |
id |
doaj-4279483ab45245c190637a204624d448 |
---|---|
record_format |
Article |
spelling |
doaj-4279483ab45245c190637a204624d4482020-11-25T02:06:35ZengBMCBMC Plant Biology1471-22292020-10-0120111310.1186/s12870-020-02662-3Transcription strategies related to photosynthesis and nitrogen metabolism of wheat in response to nitrogen deficiencyXin Liu0Chengmiao Yin1Li Xiang2Weitao Jiang3Shaozhuo Xu4Zhiquan Mao5State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural UniversityState Key Laboratory of Crop Biology, College of Horticultural Science and Engineering, Shandong Agricultural UniversityState Key Laboratory of Crop Biology, College of Horticultural Science and Engineering, Shandong Agricultural UniversityState Key Laboratory of Crop Biology, College of Horticultural Science and Engineering, Shandong Agricultural UniversityState Key Laboratory of Crop Biology, College of Horticultural Science and Engineering, Shandong Agricultural UniversityState Key Laboratory of Crop Biology, College of Horticultural Science and Engineering, Shandong Agricultural UniversityAbstract Background Agricultural yield is closely associated with nitrogen application. Thus, reducing the application of nitrogen without affecting agricultural production remains a challenging task. To understand the metabolic, physiological, and morphological response of wheat (Triticum aestivum) to nitrogen deficiency, it is crucial to identify the genes involved in the activated signaling pathways. Results We conducted a hydroponic experiment using a complete nutrient solution (N1) and a nutrient solution without nitrogen (N0). Wheat plants under nitrogen-deficient conditions (NDC) showed decreased crop height, leaf area, root volume, photosynthetic rate, crop weight, and increased root length, root surface area, root/shoot ratio. It indicates that nitrogen deficiency altered the phenotype of wheat plants. Furthermore, we performed a comprehensive analysis of the phenotype, transcriptome, GO pathways, and KEGG pathways of DEGs identified in wheat grown under NDC. It showed up-regulation of Exp (24), and Nrt (9) gene family members, which increased the nitrogen absorption and down-regulation of Pet (3), Psb (8), Nar (3), and Nir (1) gene family members hampered photosynthesis and nitrogen metabolism. Conclusions We identified 48 candidate genes that were involved in improved photosynthesis and nitrogen metabolism in wheat plants grown under NDC. These genes may serve as molecular markers for genetic breeding of crops.http://link.springer.com/article/10.1186/s12870-020-02662-3Nitrogen deficiencyNitrogen metabolismPhotosynthesisTranscriptomeWheat |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xin Liu Chengmiao Yin Li Xiang Weitao Jiang Shaozhuo Xu Zhiquan Mao |
spellingShingle |
Xin Liu Chengmiao Yin Li Xiang Weitao Jiang Shaozhuo Xu Zhiquan Mao Transcription strategies related to photosynthesis and nitrogen metabolism of wheat in response to nitrogen deficiency BMC Plant Biology Nitrogen deficiency Nitrogen metabolism Photosynthesis Transcriptome Wheat |
author_facet |
Xin Liu Chengmiao Yin Li Xiang Weitao Jiang Shaozhuo Xu Zhiquan Mao |
author_sort |
Xin Liu |
title |
Transcription strategies related to photosynthesis and nitrogen metabolism of wheat in response to nitrogen deficiency |
title_short |
Transcription strategies related to photosynthesis and nitrogen metabolism of wheat in response to nitrogen deficiency |
title_full |
Transcription strategies related to photosynthesis and nitrogen metabolism of wheat in response to nitrogen deficiency |
title_fullStr |
Transcription strategies related to photosynthesis and nitrogen metabolism of wheat in response to nitrogen deficiency |
title_full_unstemmed |
Transcription strategies related to photosynthesis and nitrogen metabolism of wheat in response to nitrogen deficiency |
title_sort |
transcription strategies related to photosynthesis and nitrogen metabolism of wheat in response to nitrogen deficiency |
publisher |
BMC |
series |
BMC Plant Biology |
issn |
1471-2229 |
publishDate |
2020-10-01 |
description |
Abstract Background Agricultural yield is closely associated with nitrogen application. Thus, reducing the application of nitrogen without affecting agricultural production remains a challenging task. To understand the metabolic, physiological, and morphological response of wheat (Triticum aestivum) to nitrogen deficiency, it is crucial to identify the genes involved in the activated signaling pathways. Results We conducted a hydroponic experiment using a complete nutrient solution (N1) and a nutrient solution without nitrogen (N0). Wheat plants under nitrogen-deficient conditions (NDC) showed decreased crop height, leaf area, root volume, photosynthetic rate, crop weight, and increased root length, root surface area, root/shoot ratio. It indicates that nitrogen deficiency altered the phenotype of wheat plants. Furthermore, we performed a comprehensive analysis of the phenotype, transcriptome, GO pathways, and KEGG pathways of DEGs identified in wheat grown under NDC. It showed up-regulation of Exp (24), and Nrt (9) gene family members, which increased the nitrogen absorption and down-regulation of Pet (3), Psb (8), Nar (3), and Nir (1) gene family members hampered photosynthesis and nitrogen metabolism. Conclusions We identified 48 candidate genes that were involved in improved photosynthesis and nitrogen metabolism in wheat plants grown under NDC. These genes may serve as molecular markers for genetic breeding of crops. |
topic |
Nitrogen deficiency Nitrogen metabolism Photosynthesis Transcriptome Wheat |
url |
http://link.springer.com/article/10.1186/s12870-020-02662-3 |
work_keys_str_mv |
AT xinliu transcriptionstrategiesrelatedtophotosynthesisandnitrogenmetabolismofwheatinresponsetonitrogendeficiency AT chengmiaoyin transcriptionstrategiesrelatedtophotosynthesisandnitrogenmetabolismofwheatinresponsetonitrogendeficiency AT lixiang transcriptionstrategiesrelatedtophotosynthesisandnitrogenmetabolismofwheatinresponsetonitrogendeficiency AT weitaojiang transcriptionstrategiesrelatedtophotosynthesisandnitrogenmetabolismofwheatinresponsetonitrogendeficiency AT shaozhuoxu transcriptionstrategiesrelatedtophotosynthesisandnitrogenmetabolismofwheatinresponsetonitrogendeficiency AT zhiquanmao transcriptionstrategiesrelatedtophotosynthesisandnitrogenmetabolismofwheatinresponsetonitrogendeficiency |
_version_ |
1724933152117358592 |