Curcumin Treatment Improves Motor Behavior in α-Synuclein Transgenic Mice.
The curry spice curcumin plays a protective role in mouse models of neurodegenerative diseases, and can also directly modulate aggregation of α-synuclein protein in vitro, yet no studies have described the interaction of curcumin and α-synuclein in genetic synucleinopathy mouse models. Here we exami...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2015-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4452784?pdf=render |
id |
doaj-427c2f76a25c48b2aca9845e2458712b |
---|---|
record_format |
Article |
spelling |
doaj-427c2f76a25c48b2aca9845e2458712b2020-11-24T20:50:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01106e012851010.1371/journal.pone.0128510Curcumin Treatment Improves Motor Behavior in α-Synuclein Transgenic Mice.Kateri J SpinelliValerie R OsterbergCharles K MeshulAmala SoumyanathVivek K UnniThe curry spice curcumin plays a protective role in mouse models of neurodegenerative diseases, and can also directly modulate aggregation of α-synuclein protein in vitro, yet no studies have described the interaction of curcumin and α-synuclein in genetic synucleinopathy mouse models. Here we examined the effect of chronic and acute curcumin treatment in the Syn-GFP mouse line, which overexpresses wild-type human α-synuclein protein. We discovered that curcumin diet intervention significantly improved gait impairments and resulted in an increase in phosphorylated forms of α-synuclein at cortical presynaptic terminals. Acute curcumin treatment also caused an increase in phosphorylated α-synuclein in terminals, but had no direct effect on α-synuclein aggregation, as measured by in vivo multiphoton imaging and Proteinase-K digestion. Using LC-MS/MS, we detected ~5 ng/mL and ~12 ng/mL free curcumin in the plasma of chronic or acutely treated mice, with a glucuronidation rate of 94% and 97%, respectively. Despite the low plasma levels and extensive metabolism of curcumin, these results show that dietary curcumin intervention correlates with significant behavioral and molecular changes in a genetic synucleinopathy mouse model that mimics human disease.http://europepmc.org/articles/PMC4452784?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kateri J Spinelli Valerie R Osterberg Charles K Meshul Amala Soumyanath Vivek K Unni |
spellingShingle |
Kateri J Spinelli Valerie R Osterberg Charles K Meshul Amala Soumyanath Vivek K Unni Curcumin Treatment Improves Motor Behavior in α-Synuclein Transgenic Mice. PLoS ONE |
author_facet |
Kateri J Spinelli Valerie R Osterberg Charles K Meshul Amala Soumyanath Vivek K Unni |
author_sort |
Kateri J Spinelli |
title |
Curcumin Treatment Improves Motor Behavior in α-Synuclein Transgenic Mice. |
title_short |
Curcumin Treatment Improves Motor Behavior in α-Synuclein Transgenic Mice. |
title_full |
Curcumin Treatment Improves Motor Behavior in α-Synuclein Transgenic Mice. |
title_fullStr |
Curcumin Treatment Improves Motor Behavior in α-Synuclein Transgenic Mice. |
title_full_unstemmed |
Curcumin Treatment Improves Motor Behavior in α-Synuclein Transgenic Mice. |
title_sort |
curcumin treatment improves motor behavior in α-synuclein transgenic mice. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
The curry spice curcumin plays a protective role in mouse models of neurodegenerative diseases, and can also directly modulate aggregation of α-synuclein protein in vitro, yet no studies have described the interaction of curcumin and α-synuclein in genetic synucleinopathy mouse models. Here we examined the effect of chronic and acute curcumin treatment in the Syn-GFP mouse line, which overexpresses wild-type human α-synuclein protein. We discovered that curcumin diet intervention significantly improved gait impairments and resulted in an increase in phosphorylated forms of α-synuclein at cortical presynaptic terminals. Acute curcumin treatment also caused an increase in phosphorylated α-synuclein in terminals, but had no direct effect on α-synuclein aggregation, as measured by in vivo multiphoton imaging and Proteinase-K digestion. Using LC-MS/MS, we detected ~5 ng/mL and ~12 ng/mL free curcumin in the plasma of chronic or acutely treated mice, with a glucuronidation rate of 94% and 97%, respectively. Despite the low plasma levels and extensive metabolism of curcumin, these results show that dietary curcumin intervention correlates with significant behavioral and molecular changes in a genetic synucleinopathy mouse model that mimics human disease. |
url |
http://europepmc.org/articles/PMC4452784?pdf=render |
work_keys_str_mv |
AT katerijspinelli curcumintreatmentimprovesmotorbehaviorinasynucleintransgenicmice AT valerierosterberg curcumintreatmentimprovesmotorbehaviorinasynucleintransgenicmice AT charleskmeshul curcumintreatmentimprovesmotorbehaviorinasynucleintransgenicmice AT amalasoumyanath curcumintreatmentimprovesmotorbehaviorinasynucleintransgenicmice AT vivekkunni curcumintreatmentimprovesmotorbehaviorinasynucleintransgenicmice |
_version_ |
1716805161998876672 |