The Association of Multiple Gene Variants with Ageing Skeletal Muscle Phenotypes in Elderly Women
There is a scarcity of studies that have investigated the role of multiple single nucleotide polymorphisms (SNPs) on a range of muscle phenotypes in an elderly population. The present study investigated the possible association of 24 SNPs with skeletal muscle phenotypes in 307 elderly Caucasian wome...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-12-01
|
Series: | Genes |
Subjects: | |
Online Access: | https://www.mdpi.com/2073-4425/11/12/1459 |
id |
doaj-42a3d395ba984dbf91fdb57205e2c0f3 |
---|---|
record_format |
Article |
spelling |
doaj-42a3d395ba984dbf91fdb57205e2c0f32020-12-06T00:00:38ZengMDPI AGGenes2073-44252020-12-01111459145910.3390/genes11121459The Association of Multiple Gene Variants with Ageing Skeletal Muscle Phenotypes in Elderly WomenPraval Khanal0Lingxiao He1Adam J. Herbert2Georgina K. Stebbings3Gladys L. Onambele-Pearson4Hans Degens5Christopher I. Morse6Martine Thomis7Alun G. Williams8Musculoskeletal Science and Sports Medicine Research Centre, Department of Sport and Exercise Sciences, Manchester Metropolitan University, Manchester M15 6BH, UKMusculoskeletal Science and Sports Medicine Research Centre, Department of Sport and Exercise Sciences, Manchester Metropolitan University, Manchester M15 6BH, UKDepartment of Sport and Exercise, Birmingham City University, Birmingham B5 5JU, UKMusculoskeletal Science and Sports Medicine Research Centre, Department of Sport and Exercise Sciences, Manchester Metropolitan University, Manchester M15 6BH, UKMusculoskeletal Science and Sports Medicine Research Centre, Department of Sport and Exercise Sciences, Manchester Metropolitan University, Manchester M15 6BH, UKDepartment of Life Sciences, Manchester Metropolitan University, Manchester M15 6BH, UKMusculoskeletal Science and Sports Medicine Research Centre, Department of Sport and Exercise Sciences, Manchester Metropolitan University, Manchester M15 6BH, UKDepartment of Movement Sciences, Physical Activity, Sports & Health Research Group, KU Leuven, 3001 Leuven, BelgiumMusculoskeletal Science and Sports Medicine Research Centre, Department of Sport and Exercise Sciences, Manchester Metropolitan University, Manchester M15 6BH, UKThere is a scarcity of studies that have investigated the role of multiple single nucleotide polymorphisms (SNPs) on a range of muscle phenotypes in an elderly population. The present study investigated the possible association of 24 SNPs with skeletal muscle phenotypes in 307 elderly Caucasian women (aged 60–91 years, 66.3 ± 11.3 kg). Skeletal muscle phenotypes included biceps brachii thickness, vastus lateralis cross-sectional areas, maximal hand grip strength, isometric knee extension and elbow flexion torque. Genotyping for 24 SNPs, chosen on their skeletal muscle structural or functional links, was conducted on DNA extracted from blood or saliva. Of the 24 SNPs, 10 were associated with at least one skeletal muscle phenotype. <i>HIF1A</i> rs11549465 was associated with three skeletal muscle phenotypes and <i>PTK2</i> rs7460 and <i>ACVR1B</i> rs10783485 were each associated with two phenotypes. <i>PTK2</i> rs7843014, <i>COL1A1</i> rs1800012, <i>CNTF</i> rs1800169, <i>NOS3</i> rs1799983, <i>MSTN</i> rs1805086, <i>TRHR</i> rs7832552 and <i>FTO</i> rs9939609 were each associated with one. Elderly women possessing favourable genotypes were 3.6–13.2% stronger and had 4.6–14.7% larger muscle than those with less favourable genotypes. These associations, together with future work involving a broader range of SNPs, may help identify individuals at particular risk of an age-associated loss of independence.https://www.mdpi.com/2073-4425/11/12/1459single nucleotide polymorphismsneuromuscularelderlygenotyping |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Praval Khanal Lingxiao He Adam J. Herbert Georgina K. Stebbings Gladys L. Onambele-Pearson Hans Degens Christopher I. Morse Martine Thomis Alun G. Williams |
spellingShingle |
Praval Khanal Lingxiao He Adam J. Herbert Georgina K. Stebbings Gladys L. Onambele-Pearson Hans Degens Christopher I. Morse Martine Thomis Alun G. Williams The Association of Multiple Gene Variants with Ageing Skeletal Muscle Phenotypes in Elderly Women Genes single nucleotide polymorphisms neuromuscular elderly genotyping |
author_facet |
Praval Khanal Lingxiao He Adam J. Herbert Georgina K. Stebbings Gladys L. Onambele-Pearson Hans Degens Christopher I. Morse Martine Thomis Alun G. Williams |
author_sort |
Praval Khanal |
title |
The Association of Multiple Gene Variants with Ageing Skeletal Muscle Phenotypes in Elderly Women |
title_short |
The Association of Multiple Gene Variants with Ageing Skeletal Muscle Phenotypes in Elderly Women |
title_full |
The Association of Multiple Gene Variants with Ageing Skeletal Muscle Phenotypes in Elderly Women |
title_fullStr |
The Association of Multiple Gene Variants with Ageing Skeletal Muscle Phenotypes in Elderly Women |
title_full_unstemmed |
The Association of Multiple Gene Variants with Ageing Skeletal Muscle Phenotypes in Elderly Women |
title_sort |
association of multiple gene variants with ageing skeletal muscle phenotypes in elderly women |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2020-12-01 |
description |
There is a scarcity of studies that have investigated the role of multiple single nucleotide polymorphisms (SNPs) on a range of muscle phenotypes in an elderly population. The present study investigated the possible association of 24 SNPs with skeletal muscle phenotypes in 307 elderly Caucasian women (aged 60–91 years, 66.3 ± 11.3 kg). Skeletal muscle phenotypes included biceps brachii thickness, vastus lateralis cross-sectional areas, maximal hand grip strength, isometric knee extension and elbow flexion torque. Genotyping for 24 SNPs, chosen on their skeletal muscle structural or functional links, was conducted on DNA extracted from blood or saliva. Of the 24 SNPs, 10 were associated with at least one skeletal muscle phenotype. <i>HIF1A</i> rs11549465 was associated with three skeletal muscle phenotypes and <i>PTK2</i> rs7460 and <i>ACVR1B</i> rs10783485 were each associated with two phenotypes. <i>PTK2</i> rs7843014, <i>COL1A1</i> rs1800012, <i>CNTF</i> rs1800169, <i>NOS3</i> rs1799983, <i>MSTN</i> rs1805086, <i>TRHR</i> rs7832552 and <i>FTO</i> rs9939609 were each associated with one. Elderly women possessing favourable genotypes were 3.6–13.2% stronger and had 4.6–14.7% larger muscle than those with less favourable genotypes. These associations, together with future work involving a broader range of SNPs, may help identify individuals at particular risk of an age-associated loss of independence. |
topic |
single nucleotide polymorphisms neuromuscular elderly genotyping |
url |
https://www.mdpi.com/2073-4425/11/12/1459 |
work_keys_str_mv |
AT pravalkhanal theassociationofmultiplegenevariantswithageingskeletalmusclephenotypesinelderlywomen AT lingxiaohe theassociationofmultiplegenevariantswithageingskeletalmusclephenotypesinelderlywomen AT adamjherbert theassociationofmultiplegenevariantswithageingskeletalmusclephenotypesinelderlywomen AT georginakstebbings theassociationofmultiplegenevariantswithageingskeletalmusclephenotypesinelderlywomen AT gladyslonambelepearson theassociationofmultiplegenevariantswithageingskeletalmusclephenotypesinelderlywomen AT hansdegens theassociationofmultiplegenevariantswithageingskeletalmusclephenotypesinelderlywomen AT christopherimorse theassociationofmultiplegenevariantswithageingskeletalmusclephenotypesinelderlywomen AT martinethomis theassociationofmultiplegenevariantswithageingskeletalmusclephenotypesinelderlywomen AT alungwilliams theassociationofmultiplegenevariantswithageingskeletalmusclephenotypesinelderlywomen AT pravalkhanal associationofmultiplegenevariantswithageingskeletalmusclephenotypesinelderlywomen AT lingxiaohe associationofmultiplegenevariantswithageingskeletalmusclephenotypesinelderlywomen AT adamjherbert associationofmultiplegenevariantswithageingskeletalmusclephenotypesinelderlywomen AT georginakstebbings associationofmultiplegenevariantswithageingskeletalmusclephenotypesinelderlywomen AT gladyslonambelepearson associationofmultiplegenevariantswithageingskeletalmusclephenotypesinelderlywomen AT hansdegens associationofmultiplegenevariantswithageingskeletalmusclephenotypesinelderlywomen AT christopherimorse associationofmultiplegenevariantswithageingskeletalmusclephenotypesinelderlywomen AT martinethomis associationofmultiplegenevariantswithageingskeletalmusclephenotypesinelderlywomen AT alungwilliams associationofmultiplegenevariantswithageingskeletalmusclephenotypesinelderlywomen |
_version_ |
1724399548671983616 |