Chemosystematic of Enterobacteriaceae Familia Obtained from Blood Cultures Based on Total Protein Profiles
The purpose of this study was to determine the chemosystematic of 14 strains of bacteria in blood cultures from Semarang using 1 reference strain S. typhi NCTC 786, based on the total protein profi les with the similarity relationship analysis based on Simple Matching Coeffi cient (SSM) analysis and...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Universitas Gadjah Mada, Yogyakarta
2013-06-01
|
Series: | Indonesian Journal of Biotechnology |
Subjects: | |
Online Access: | https://jurnal.ugm.ac.id/ijbiotech/article/view/7862 |
id |
doaj-42a86f807ade4e4cac879856872ce0aa |
---|---|
record_format |
Article |
spelling |
doaj-42a86f807ade4e4cac879856872ce0aa2020-11-24T21:59:21ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412013-06-01181586310.22146/ijbiotech.78626578Chemosystematic of Enterobacteriaceae Familia Obtained from Blood Cultures Based on Total Protein ProfilesSri Darmawati0Langkah Sembiring1Widya Asmara2Wayan T. Artama3Syaiful Anwar4Laboratory of Microbiology, Faculty of Nursing and Health, Universitas Muhammadiyah Semarang, SemarangLaboratory of Microbiology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta3Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, YogyakartaDepartment of Biochemistry, Faculty of Veterinary Medicine, Universitas Gadjah Mada, YogyakartaFaculty of Animal Husbandry, Universitas Diponegoro, SemarangThe purpose of this study was to determine the chemosystematic of 14 strains of bacteria in blood cultures from Semarang using 1 reference strain S. typhi NCTC 786, based on the total protein profi les with the similarity relationship analysis based on Simple Matching Coeffi cient (SSM) analysis and algorithm methodof unweighted pair group with averages (UPGMA) presented in a dendrogram. The results showed that thechemosystematic based on the total protein profi les using SDS-PAGE method can classify the member ofbacterial strains of each species. The Clusters respectively consist of 4 strains of S. typhi (similarity: 89.7%),2 strains of Ser. marcescens (similarity: 89.7%), two strains of E. coli, and one strain of Salmonella ssp, S. typhi NCTC 786 (similarity: 100%). Those three incorporated clusters had the similarity value of 75.3%. Those four strains of Ent. cloacae composed in one cluster (similarity: 100%) are incorporated in a cluster consisting of one strain of Kleb. pneumoniae (similarity: 92.9%). Both clusters were incorporated in a cluster consisting of S. typhi NCTC 786 (similarity: 67.9%). Key words: Enterobacteriaceae, chemosystematic, blood cultures, protein profilehttps://jurnal.ugm.ac.id/ijbiotech/article/view/7862EnterobacteriaceaeChemosystematicBlood CulturesProtein profile |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sri Darmawati Langkah Sembiring Widya Asmara Wayan T. Artama Syaiful Anwar |
spellingShingle |
Sri Darmawati Langkah Sembiring Widya Asmara Wayan T. Artama Syaiful Anwar Chemosystematic of Enterobacteriaceae Familia Obtained from Blood Cultures Based on Total Protein Profiles Indonesian Journal of Biotechnology Enterobacteriaceae Chemosystematic Blood Cultures Protein profile |
author_facet |
Sri Darmawati Langkah Sembiring Widya Asmara Wayan T. Artama Syaiful Anwar |
author_sort |
Sri Darmawati |
title |
Chemosystematic of Enterobacteriaceae Familia Obtained from Blood Cultures Based on Total Protein Profiles |
title_short |
Chemosystematic of Enterobacteriaceae Familia Obtained from Blood Cultures Based on Total Protein Profiles |
title_full |
Chemosystematic of Enterobacteriaceae Familia Obtained from Blood Cultures Based on Total Protein Profiles |
title_fullStr |
Chemosystematic of Enterobacteriaceae Familia Obtained from Blood Cultures Based on Total Protein Profiles |
title_full_unstemmed |
Chemosystematic of Enterobacteriaceae Familia Obtained from Blood Cultures Based on Total Protein Profiles |
title_sort |
chemosystematic of enterobacteriaceae familia obtained from blood cultures based on total protein profiles |
publisher |
Universitas Gadjah Mada, Yogyakarta |
series |
Indonesian Journal of Biotechnology |
issn |
0853-8654 2089-2241 |
publishDate |
2013-06-01 |
description |
The purpose of this study was to determine the chemosystematic of 14 strains of bacteria in blood cultures from Semarang using 1 reference strain S. typhi NCTC 786, based on the total protein profi les with the similarity relationship analysis based on Simple Matching Coeffi cient (SSM) analysis and algorithm methodof unweighted pair group with averages (UPGMA) presented in a dendrogram. The results showed that thechemosystematic based on the total protein profi les using SDS-PAGE method can classify the member ofbacterial strains of each species. The Clusters respectively consist of 4 strains of S. typhi (similarity: 89.7%),2 strains of Ser. marcescens (similarity: 89.7%), two strains of E. coli, and one strain of Salmonella ssp, S. typhi NCTC 786 (similarity: 100%). Those three incorporated clusters had the similarity value of 75.3%. Those four strains of Ent. cloacae composed in one cluster (similarity: 100%) are incorporated in a cluster consisting of one strain of Kleb. pneumoniae (similarity: 92.9%). Both clusters were incorporated in a cluster consisting of S. typhi NCTC 786 (similarity: 67.9%). Key words: Enterobacteriaceae, chemosystematic, blood cultures, protein profile |
topic |
Enterobacteriaceae Chemosystematic Blood Cultures Protein profile |
url |
https://jurnal.ugm.ac.id/ijbiotech/article/view/7862 |
work_keys_str_mv |
AT sridarmawati chemosystematicofenterobacteriaceaefamiliaobtainedfrombloodculturesbasedontotalproteinprofiles AT langkahsembiring chemosystematicofenterobacteriaceaefamiliaobtainedfrombloodculturesbasedontotalproteinprofiles AT widyaasmara chemosystematicofenterobacteriaceaefamiliaobtainedfrombloodculturesbasedontotalproteinprofiles AT wayantartama chemosystematicofenterobacteriaceaefamiliaobtainedfrombloodculturesbasedontotalproteinprofiles AT syaifulanwar chemosystematicofenterobacteriaceaefamiliaobtainedfrombloodculturesbasedontotalproteinprofiles |
_version_ |
1725847698345558016 |