Comparison of the performance of a chemiluminescence assay and an ELISA for detection of anti-GBM antibodies
Objective Autoantibodies to the α3 chain noncollagen 1 domain of type IV collagen (α3(IV)NC1) are a serological hallmark in the diagnosis of anti-glomerular basement membrane (GBM) disease. The objective of our study was to compare the performance of anti-glomerular basement membrane (GBM) antibody...
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2020-01-01
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Online Access: | http://dx.doi.org/10.1080/0886022X.2019.1702056 |
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doaj-42abeb4e687f493ab85510f42ba038532021-03-18T14:42:05ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492020-01-01421485310.1080/0886022X.2019.17020561702056Comparison of the performance of a chemiluminescence assay and an ELISA for detection of anti-GBM antibodiesYing Tan0Wei Pang1Xiaoyu Jia2Ming-hui Zhao3Renal Division, Department of Medicine, Peking University First HospitalRenal Division, Department of Medicine, Peking University First HospitalRenal Division, Department of Medicine, Peking University First HospitalRenal Division, Department of Medicine, Peking University First HospitalObjective Autoantibodies to the α3 chain noncollagen 1 domain of type IV collagen (α3(IV)NC1) are a serological hallmark in the diagnosis of anti-glomerular basement membrane (GBM) disease. The objective of our study was to compare the performance of anti-glomerular basement membrane (GBM) antibody detection by chemiluminescence immunoassay (CIA) and by enzyme-linked immunosorbent assays (ELISAs). Methods Sera from outpatients who were suspected to have anti-GBM disease and 31 patients with biopsy-proven anti-GBM disease were collected. Thirty normal controls were also included. All samples were tested for anti-GBM antibodies by CIA and commercial ELISA. The anti-GBM antibody-positive samples were confirmed by a homemade ELISA coated with recombinant human α3(IV)NC1. Results Compared with detection of anti-GBM antibodies with ELISA, detection of anti-GBM antibodies with CIA showed a positivity agreement of 70% and a negativity agreement of 98.6%. Among the 4 patients with different results, the anti-GBM antibody detection by CIA was in agreement with the homemade ELISA coated with recombinant human α3(IV)NC1 and the clinical diagnosis. In 31 patients with anti-GBM disease, good agreement was achieved in the detection of anti-GBM antibodies with CIA, commercial ELISA and the homemade ELISA (100%, 100%). The AUC for CIA and commercial ELISA was 0.987 and 0.966, respectively. Conclusions The detection of anti-GBM antibodies with CIA demonstrated good sensitivity and specificity and was in good agreement with our homemade ELISA, which seems better than the commercial ELISA in suspected anti-GBM disease patients. The three assays performed in parallel in the diagnosis of anti-GBM disease patients.http://dx.doi.org/10.1080/0886022X.2019.1702056autoantibodiesgbmgoodpasture’s syndromerapidly progressive crescentic glomerulonephritis (rpgn) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ying Tan Wei Pang Xiaoyu Jia Ming-hui Zhao |
spellingShingle |
Ying Tan Wei Pang Xiaoyu Jia Ming-hui Zhao Comparison of the performance of a chemiluminescence assay and an ELISA for detection of anti-GBM antibodies Renal Failure autoantibodies gbm goodpasture’s syndrome rapidly progressive crescentic glomerulonephritis (rpgn) |
author_facet |
Ying Tan Wei Pang Xiaoyu Jia Ming-hui Zhao |
author_sort |
Ying Tan |
title |
Comparison of the performance of a chemiluminescence assay and an ELISA for detection of anti-GBM antibodies |
title_short |
Comparison of the performance of a chemiluminescence assay and an ELISA for detection of anti-GBM antibodies |
title_full |
Comparison of the performance of a chemiluminescence assay and an ELISA for detection of anti-GBM antibodies |
title_fullStr |
Comparison of the performance of a chemiluminescence assay and an ELISA for detection of anti-GBM antibodies |
title_full_unstemmed |
Comparison of the performance of a chemiluminescence assay and an ELISA for detection of anti-GBM antibodies |
title_sort |
comparison of the performance of a chemiluminescence assay and an elisa for detection of anti-gbm antibodies |
publisher |
Taylor & Francis Group |
series |
Renal Failure |
issn |
0886-022X 1525-6049 |
publishDate |
2020-01-01 |
description |
Objective Autoantibodies to the α3 chain noncollagen 1 domain of type IV collagen (α3(IV)NC1) are a serological hallmark in the diagnosis of anti-glomerular basement membrane (GBM) disease. The objective of our study was to compare the performance of anti-glomerular basement membrane (GBM) antibody detection by chemiluminescence immunoassay (CIA) and by enzyme-linked immunosorbent assays (ELISAs). Methods Sera from outpatients who were suspected to have anti-GBM disease and 31 patients with biopsy-proven anti-GBM disease were collected. Thirty normal controls were also included. All samples were tested for anti-GBM antibodies by CIA and commercial ELISA. The anti-GBM antibody-positive samples were confirmed by a homemade ELISA coated with recombinant human α3(IV)NC1. Results Compared with detection of anti-GBM antibodies with ELISA, detection of anti-GBM antibodies with CIA showed a positivity agreement of 70% and a negativity agreement of 98.6%. Among the 4 patients with different results, the anti-GBM antibody detection by CIA was in agreement with the homemade ELISA coated with recombinant human α3(IV)NC1 and the clinical diagnosis. In 31 patients with anti-GBM disease, good agreement was achieved in the detection of anti-GBM antibodies with CIA, commercial ELISA and the homemade ELISA (100%, 100%). The AUC for CIA and commercial ELISA was 0.987 and 0.966, respectively. Conclusions The detection of anti-GBM antibodies with CIA demonstrated good sensitivity and specificity and was in good agreement with our homemade ELISA, which seems better than the commercial ELISA in suspected anti-GBM disease patients. The three assays performed in parallel in the diagnosis of anti-GBM disease patients. |
topic |
autoantibodies gbm goodpasture’s syndrome rapidly progressive crescentic glomerulonephritis (rpgn) |
url |
http://dx.doi.org/10.1080/0886022X.2019.1702056 |
work_keys_str_mv |
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