A flow cytometry-based FRET assay to identify and analyse protein-protein interactions in living cells.
Försters resonance energy transfer (FRET) microscopy is widely used for the analysis of protein interactions in intact cells. However, FRET microscopy is technically challenging and does not allow assessing interactions in large cell numbers. To overcome these limitations we developed a flow cytomet...
Main Authors: | Carina Banning, Jörg Votteler, Dirk Hoffmann, Herwig Koppensteiner, Martin Warmer, Rudolph Reimer, Frank Kirchhoff, Ulrich Schubert, Joachim Hauber, Michael Schindler |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2010-02-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC2825263?pdf=render |
Similar Items
-
Analysis of IFITM-IFITM Interactions by a Flow Cytometry-Based FRET Assay
by: Michael Winkler, et al.
Published: (2019-08-01) -
Formation of trans-activation competent HIV-1 Rev:RRE complexes requires the recruitment of multiple protein activation domains.
by: Dirk Hoffmann, et al.
Published: (2012-01-01) -
Practical and reliable FRET/FLIM pair of fluorescent proteins
by: Shemiakina Irina I, et al.
Published: (2009-03-01) -
Vpu serine 52 dependent counteraction of tetherin is required for HIV-1 replication in macrophages, but not in ex vivo human lymphoid tissue
by: Specht Anke, et al.
Published: (2010-01-01) -
Solution structure of the equine infectious anemia virus p9 protein: a rationalization of its different ALIX binding requirements compared to the analogous HIV-p6 protein
by: Henklein Peter, et al.
Published: (2009-09-01)