De Novo A-to-I RNA Editing Discovery in lncRNA
Background: Adenosine to inosine (A-to-I) RNA editing is the most frequent editing event in humans. It converts adenosine to inosine in double-stranded RNA regions (in coding and non-coding RNAs) through the action of the adenosine deaminase acting on RNA (ADAR) enzymes. Long non-coding RNAs, partic...
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MDPI AG
2020-10-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/12/10/2959 |
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doaj-42c1166efe9740b180de5176832e1d2d2020-11-25T03:38:32ZengMDPI AGCancers2072-66942020-10-01122959295910.3390/cancers12102959De Novo A-to-I RNA Editing Discovery in lncRNADomenico Alessandro Silvestris0Chiara Scopa1Sara Hanchi2Franco Locatelli3Angela Gallo4RNA Editing Lab, Department of Hematology and Oncology and Cell and Gene Therapy, Bambino Gesù Children’s Hospital (OPBG), IRCCS, 00146 Rome, ItalyRNA Editing Lab, Department of Hematology and Oncology and Cell and Gene Therapy, Bambino Gesù Children’s Hospital (OPBG), IRCCS, 00146 Rome, ItalyRNA Editing Lab, Department of Hematology and Oncology and Cell and Gene Therapy, Bambino Gesù Children’s Hospital (OPBG), IRCCS, 00146 Rome, ItalyRNA Editing Lab, Department of Hematology and Oncology and Cell and Gene Therapy, Bambino Gesù Children’s Hospital (OPBG), IRCCS, 00146 Rome, ItalyRNA Editing Lab, Department of Hematology and Oncology and Cell and Gene Therapy, Bambino Gesù Children’s Hospital (OPBG), IRCCS, 00146 Rome, ItalyBackground: Adenosine to inosine (A-to-I) RNA editing is the most frequent editing event in humans. It converts adenosine to inosine in double-stranded RNA regions (in coding and non-coding RNAs) through the action of the adenosine deaminase acting on RNA (ADAR) enzymes. Long non-coding RNAs, particularly abundant in the brain, account for a large fraction of the human transcriptome, and their important regulatory role is becoming progressively evident in both normal and transformed cells. Results: Herein, we present a bioinformatic analysis to generate a comprehensive inosinome picture in long non-coding RNAs (lncRNAs), using an ad hoc index and searching for de novo editing events in the normal brain cortex as well as in glioblastoma, a highly aggressive human brain cancer. We discovered >10,000 new sites and 335 novel lncRNAs that undergo editing, never reported before. We found a generalized downregulation of editing at multiple lncRNA sites in glioblastoma samples when compared to the normal brain cortex. Conclusion: Overall, our study discloses a novel layer of complexity that controls lncRNAs in the brain and brain cancer.https://www.mdpi.com/2072-6694/12/10/2959RNA editinglncRNAbrainglioblastoma |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Domenico Alessandro Silvestris Chiara Scopa Sara Hanchi Franco Locatelli Angela Gallo |
| spellingShingle |
Domenico Alessandro Silvestris Chiara Scopa Sara Hanchi Franco Locatelli Angela Gallo De Novo A-to-I RNA Editing Discovery in lncRNA Cancers RNA editing lncRNA brain glioblastoma |
| author_facet |
Domenico Alessandro Silvestris Chiara Scopa Sara Hanchi Franco Locatelli Angela Gallo |
| author_sort |
Domenico Alessandro Silvestris |
| title |
De Novo A-to-I RNA Editing Discovery in lncRNA |
| title_short |
De Novo A-to-I RNA Editing Discovery in lncRNA |
| title_full |
De Novo A-to-I RNA Editing Discovery in lncRNA |
| title_fullStr |
De Novo A-to-I RNA Editing Discovery in lncRNA |
| title_full_unstemmed |
De Novo A-to-I RNA Editing Discovery in lncRNA |
| title_sort |
de novo a-to-i rna editing discovery in lncrna |
| publisher |
MDPI AG |
| series |
Cancers |
| issn |
2072-6694 |
| publishDate |
2020-10-01 |
| description |
Background: Adenosine to inosine (A-to-I) RNA editing is the most frequent editing event in humans. It converts adenosine to inosine in double-stranded RNA regions (in coding and non-coding RNAs) through the action of the adenosine deaminase acting on RNA (ADAR) enzymes. Long non-coding RNAs, particularly abundant in the brain, account for a large fraction of the human transcriptome, and their important regulatory role is becoming progressively evident in both normal and transformed cells. Results: Herein, we present a bioinformatic analysis to generate a comprehensive inosinome picture in long non-coding RNAs (lncRNAs), using an ad hoc index and searching for de novo editing events in the normal brain cortex as well as in glioblastoma, a highly aggressive human brain cancer. We discovered >10,000 new sites and 335 novel lncRNAs that undergo editing, never reported before. We found a generalized downregulation of editing at multiple lncRNA sites in glioblastoma samples when compared to the normal brain cortex. Conclusion: Overall, our study discloses a novel layer of complexity that controls lncRNAs in the brain and brain cancer. |
| topic |
RNA editing lncRNA brain glioblastoma |
| url |
https://www.mdpi.com/2072-6694/12/10/2959 |
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