Rapid and sensitive detection of ochratoxin A in rice flour using a fluorescent microsphere immunochromatographic test strip assay
In this study, a high sensitivity and specificity monoclonal antibody (mAb) was developed based on the hydroxy and carboxy-termini of ochratoxin A (OTA). A fluorescent microsphere immunochromatographic test strip (FM-ICTS) was assembled for the rapid detection the OTA in rice flour. Some relevant pa...
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Online Access: | http://dx.doi.org/10.1080/09540105.2020.1745157 |
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doaj-42ddbae9d6be473a8ed14f77e90ede602020-12-17T12:50:41ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432020-01-0131156357410.1080/09540105.2020.17451571745157Rapid and sensitive detection of ochratoxin A in rice flour using a fluorescent microsphere immunochromatographic test strip assayYuan Cheng0Liqiang Liu1Haiying Liu2Liguang Xu3Hua Kuang4State Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityIn this study, a high sensitivity and specificity monoclonal antibody (mAb) was developed based on the hydroxy and carboxy-termini of ochratoxin A (OTA). A fluorescent microsphere immunochromatographic test strip (FM-ICTS) was assembled for the rapid detection the OTA in rice flour. Some relevant parameters were optimized including the best concentration of coating antigen and optimal diluent buffer composition. Under optimal conditions, the strip assay had a visual limit of detection (LOD) of 2 ng/mL and a cut-off value of 16 ng/mL for OTA in rice flour samples. By means of a strip scan reader, we obtained a quantitative measurement for OTA with an LOD of 1.27 ng/mL. No significant difference in the strip test results was seen when compared to an ic-ELISA assay or LC–MS/MS. This also shows, therefore, that the FM-ICTS has potential application for rapid and accurate quantitative detection in the market place.http://dx.doi.org/10.1080/09540105.2020.1745157rice flourochratoxin afood safetyfluorescent microspheremonoclonal antibody |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuan Cheng Liqiang Liu Haiying Liu Liguang Xu Hua Kuang |
spellingShingle |
Yuan Cheng Liqiang Liu Haiying Liu Liguang Xu Hua Kuang Rapid and sensitive detection of ochratoxin A in rice flour using a fluorescent microsphere immunochromatographic test strip assay Food and Agricultural Immunology rice flour ochratoxin a food safety fluorescent microsphere monoclonal antibody |
author_facet |
Yuan Cheng Liqiang Liu Haiying Liu Liguang Xu Hua Kuang |
author_sort |
Yuan Cheng |
title |
Rapid and sensitive detection of ochratoxin A in rice flour using a fluorescent microsphere immunochromatographic test strip assay |
title_short |
Rapid and sensitive detection of ochratoxin A in rice flour using a fluorescent microsphere immunochromatographic test strip assay |
title_full |
Rapid and sensitive detection of ochratoxin A in rice flour using a fluorescent microsphere immunochromatographic test strip assay |
title_fullStr |
Rapid and sensitive detection of ochratoxin A in rice flour using a fluorescent microsphere immunochromatographic test strip assay |
title_full_unstemmed |
Rapid and sensitive detection of ochratoxin A in rice flour using a fluorescent microsphere immunochromatographic test strip assay |
title_sort |
rapid and sensitive detection of ochratoxin a in rice flour using a fluorescent microsphere immunochromatographic test strip assay |
publisher |
Taylor & Francis Group |
series |
Food and Agricultural Immunology |
issn |
0954-0105 1465-3443 |
publishDate |
2020-01-01 |
description |
In this study, a high sensitivity and specificity monoclonal antibody (mAb) was developed based on the hydroxy and carboxy-termini of ochratoxin A (OTA). A fluorescent microsphere immunochromatographic test strip (FM-ICTS) was assembled for the rapid detection the OTA in rice flour. Some relevant parameters were optimized including the best concentration of coating antigen and optimal diluent buffer composition. Under optimal conditions, the strip assay had a visual limit of detection (LOD) of 2 ng/mL and a cut-off value of 16 ng/mL for OTA in rice flour samples. By means of a strip scan reader, we obtained a quantitative measurement for OTA with an LOD of 1.27 ng/mL. No significant difference in the strip test results was seen when compared to an ic-ELISA assay or LC–MS/MS. This also shows, therefore, that the FM-ICTS has potential application for rapid and accurate quantitative detection in the market place. |
topic |
rice flour ochratoxin a food safety fluorescent microsphere monoclonal antibody |
url |
http://dx.doi.org/10.1080/09540105.2020.1745157 |
work_keys_str_mv |
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