Reversal of type 1 diabetes via islet β cell regeneration following immune modulation by cord blood-derived multipotent stem cells

• Main Authors: Zhao Yong, Jiang Zhaoshun, Zhao Tingbao, Ye Mingliang, Hu Chengjin, Yin Zhaohui, Li Heng, Zhang Ye, Diao Yalin, Li Yunxiang, Chen Yingjian, Sun Xiaoming, Fisk Mary, Skidgel Randal, Holterman Mark, Prabhakar Bellur, Mazzone Theodore
• Format: Article
• Language: English
• Published: BMC 2012-01-01
• Series: BMC Medicine
• Online Access: http://www.biomedcentral.com/1741-7015/10/3

<p>Abstract</p> <p>Background</p> <p>Inability to control autoimmunity is the primary barrier to developing a cure for type 1 diabetes (T1D). Evidence that human cord blood-derived multipotent stem cells (CB-SCs) can control autoimmune responses by altering regulatory T cells (Tregs) and human islet β cell-specific T cell clones offers promise for a new approach to overcome the autoimmunity underlying T1D.</p> <p>Methods</p> <p>We developed a procedure for Stem Cell Educator therapy in which a patient's blood is circulated through a closed-loop system that separates lymphocytes from the whole blood and briefly co-cultures them with adherent CB-SCs before returning them to the patient's circulation. In an open-label, phase1/phase 2 study, patients (n = 15) with T1D received one treatment with the Stem Cell Educator. Median age was 29 years (range: 15 to 41), and median diabetic history was 8 years (range: 1 to 21).</p> <p>Results</p> <p>Stem Cell Educator therapy was well tolerated in all participants with minimal pain from two venipunctures and no adverse events. Stem Cell Educator therapy can markedly improve C-peptide levels, reduce the median glycated hemoglobin A₁C (HbA₁C) values, and decrease the median daily dose of insulin in patients with some residual β cell function (n = 6) and patients with no residual pancreatic islet β cell function (n = 6). Treatment also produced an increase in basal and glucose-stimulated C-peptide levels through 40 weeks. However, participants in the Control Group (n = 3) did not exhibit significant change at any follow-up. Individuals who received Stem Cell Educator therapy exhibited increased expression of co-stimulating molecules (specifically, CD28 and ICOS), increases in the number of CD4⁺CD25⁺Foxp3⁺Tregs, and restoration of Th1/Th2/Th3 cytokine balance.</p> <p>Conclusions</p> <p>Stem Cell Educator therapy is safe, and in individuals with moderate or severe T1D, a single treatment produces lasting improvement in metabolic control. Initial results indicate Stem Cell Educator therapy reverses autoimmunity and promotes regeneration of islet β cells. Successful immune modulation by CB-SCs and the resulting clinical improvement in patient status may have important implications for other autoimmune and inflammation-related diseases without the safety and ethical concerns associated with conventional stem cell-based approaches.</p> <p>Trial registration</p> <p>ClinicalTrials.gov number, <a href="http://www.clinicaltrials.gov/ct2/show/NCT01350219">NCT01350219</a>.</p>