Isolation of plant growth-promoting Pseudomonas sp. PPR8 from the rhizosphere of Phaseolus vulgaris L.
In vitro screening of plant growth-promoting (PGP) traits was carried out using eight Pseudomonas spp., PPR1 to PPR8, isolated from the rhizosphere of Phaseolus vulgaris growing on the Uttarakhand Himalayan range in India. All the isolates were fast growers, positive for catalase, oxidase a...
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doaj-42f945d2d56b4613a846c26f4f608b3d2020-11-24T22:49:56ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392016-01-0168236337410.2298/ABS150701028K0354-46641600028KIsolation of plant growth-promoting Pseudomonas sp. PPR8 from the rhizosphere of Phaseolus vulgaris L.Kumar Pankaj0Dubey Ramesh Chandra1Maheshwari Dinesh Kumar2Park Yong-Ha3Bajpai Vivek K.4Gurukul Kangri University, Department of Botany and Microbiology, Haridwar, Uttrakhand, India + Dolphin (PG) College of Science & Agriculture, Department of Microbiology, Chunni Kalan, Fatehgarh Sahib, Punjab, IndiaGurukul Kangri University, Department of Botany and Microbiology, Haridwar, Uttrakhand, IndiaGurukul Kangri University, Department of Botany and Microbiology, Haridwar, Uttrakhand, IndiaYeungnam University, School of Biotechnology, Department of Applied Microbiology and Biotechnology, Gyeongsan, Gyeongbuk, Republic of KoreaYeungnam University, School of Biotechnology, Department of Applied Microbiology and Biotechnology, Gyeongsan, Gyeongbuk, Republic of KoreaIn vitro screening of plant growth-promoting (PGP) traits was carried out using eight Pseudomonas spp., PPR1 to PPR8, isolated from the rhizosphere of Phaseolus vulgaris growing on the Uttarakhand Himalayan range in India. All the isolates were fast growers, positive for catalase, oxidase and urease activities, and utilized lactose and some amino acids. All the isolates were indole acetic acid (IAA) positive, however PPR8 solubilized potassium and zinc along with various other types of inorganic (tricalcium, dicalcium and zinc phosphate) and organic (calcium phytate) phosphates, as well as producing siderophore and ACC deaminase. PPR8 also produced cyanogens, extracellular chitinase, β-1,3-glucanase, β-1,4-glucanase and oxalate oxidase. Based on the PGP traits of all isolates, PPR8 was found to be the most potent plant growth-promoting rhizobacteria (PGPR). Further, PPR8 was identified as Pseudomonas sp. PPR8, based on 16S rRNA gene sequencing analysis. Moreover, the PGP activities of PPR8 confirmed it to be a potent biocontrol agent, inhibiting the growth of various plant pathogenic fungi. This study reveals the potential of Pseudomonas sp. PPR8 to be used as a good bioinoculant for growth promotion of common bean and for the protection of important legume crops from various deleterious phytopathogens.http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600028K.pdfPseudomonas sp. PPR8Phaseolus vulgarisplant growth promotionbiocontrol |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kumar Pankaj Dubey Ramesh Chandra Maheshwari Dinesh Kumar Park Yong-Ha Bajpai Vivek K. |
spellingShingle |
Kumar Pankaj Dubey Ramesh Chandra Maheshwari Dinesh Kumar Park Yong-Ha Bajpai Vivek K. Isolation of plant growth-promoting Pseudomonas sp. PPR8 from the rhizosphere of Phaseolus vulgaris L. Archives of Biological Sciences Pseudomonas sp. PPR8 Phaseolus vulgaris plant growth promotion biocontrol |
author_facet |
Kumar Pankaj Dubey Ramesh Chandra Maheshwari Dinesh Kumar Park Yong-Ha Bajpai Vivek K. |
author_sort |
Kumar Pankaj |
title |
Isolation of plant growth-promoting Pseudomonas sp. PPR8 from the rhizosphere of Phaseolus vulgaris L. |
title_short |
Isolation of plant growth-promoting Pseudomonas sp. PPR8 from the rhizosphere of Phaseolus vulgaris L. |
title_full |
Isolation of plant growth-promoting Pseudomonas sp. PPR8 from the rhizosphere of Phaseolus vulgaris L. |
title_fullStr |
Isolation of plant growth-promoting Pseudomonas sp. PPR8 from the rhizosphere of Phaseolus vulgaris L. |
title_full_unstemmed |
Isolation of plant growth-promoting Pseudomonas sp. PPR8 from the rhizosphere of Phaseolus vulgaris L. |
title_sort |
isolation of plant growth-promoting pseudomonas sp. ppr8 from the rhizosphere of phaseolus vulgaris l. |
publisher |
University of Belgrade, University of Novi Sad |
series |
Archives of Biological Sciences |
issn |
0354-4664 1821-4339 |
publishDate |
2016-01-01 |
description |
In vitro screening of plant growth-promoting (PGP) traits was carried out
using eight Pseudomonas spp., PPR1 to PPR8, isolated from the rhizosphere of
Phaseolus vulgaris growing on the Uttarakhand Himalayan range in India. All
the isolates were fast growers, positive for catalase, oxidase and urease
activities, and utilized lactose and some amino acids. All the isolates were
indole acetic acid (IAA) positive, however PPR8 solubilized potassium and
zinc along with various other types of inorganic (tricalcium, dicalcium and
zinc phosphate) and organic (calcium phytate) phosphates, as well as
producing siderophore and ACC deaminase. PPR8 also produced cyanogens,
extracellular chitinase, β-1,3-glucanase, β-1,4-glucanase and oxalate
oxidase. Based on the PGP traits of all isolates, PPR8 was found to be the
most potent plant growth-promoting rhizobacteria (PGPR). Further, PPR8 was
identified as Pseudomonas sp. PPR8, based on 16S rRNA gene sequencing
analysis. Moreover, the PGP activities of PPR8 confirmed it to be a potent
biocontrol agent, inhibiting the growth of various plant pathogenic fungi.
This study reveals the potential of Pseudomonas sp. PPR8 to be used as a
good bioinoculant for growth promotion of common bean and for the protection
of important legume crops from various deleterious phytopathogens. |
topic |
Pseudomonas sp. PPR8 Phaseolus vulgaris plant growth promotion biocontrol |
url |
http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600028K.pdf |
work_keys_str_mv |
AT kumarpankaj isolationofplantgrowthpromotingpseudomonasspppr8fromtherhizosphereofphaseolusvulgarisl AT dubeyrameshchandra isolationofplantgrowthpromotingpseudomonasspppr8fromtherhizosphereofphaseolusvulgarisl AT maheshwaridineshkumar isolationofplantgrowthpromotingpseudomonasspppr8fromtherhizosphereofphaseolusvulgarisl AT parkyongha isolationofplantgrowthpromotingpseudomonasspppr8fromtherhizosphereofphaseolusvulgarisl AT bajpaivivekk isolationofplantgrowthpromotingpseudomonasspppr8fromtherhizosphereofphaseolusvulgarisl |
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