TRIM14 promotes colorectal cancer cell migration and invasion through the SPHK1/STAT3 pathway
Abstract Background Colorectal cancer (CRC) is one of the most lethal malignancies. Tripartite Motif Containing 14 (TRIM14) is a member of TRIM family proteins, which are involved in the pathogenesis of various cancers. This study aimed to investigate TRIM14 expression in CRC tissues, and its effect...
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doaj-430241c2e2cb4c9a8232469ed85d639b2020-11-25T02:01:42ZengBMCCancer Cell International1475-28672018-12-011811910.1186/s12935-018-0701-1TRIM14 promotes colorectal cancer cell migration and invasion through the SPHK1/STAT3 pathwayZhonghai Jin0Hongguang Li1Xiaofei Hong2Guangrong Ying3Xiaofeng Lu4Lilei Zhuang5Shenbao Wu6Department of Gastroenterology, Yiwu Hospital, Wenzhou Medical UniversityDepartment of Gastroenterology, Yiwu Hospital, Wenzhou Medical UniversityDepartment of Gastroenterology, Yiwu Hospital, Wenzhou Medical UniversityDepartment of Gastroenterology, Yiwu Hospital, Wenzhou Medical UniversityDepartment of Gastroenterology, Yiwu Hospital, Wenzhou Medical UniversityDepartment of Gastroenterology, Yiwu Hospital, Wenzhou Medical UniversityDepartment of Gastroenterology, Yiwu Hospital, Wenzhou Medical UniversityAbstract Background Colorectal cancer (CRC) is one of the most lethal malignancies. Tripartite Motif Containing 14 (TRIM14) is a member of TRIM family proteins, which are involved in the pathogenesis of various cancers. This study aimed to investigate TRIM14 expression in CRC tissues, and its effects on the migration and invasion of CRC cell lines. Methods TRIM14 mRNA expression was detected by real-time PCR analysis. Cell migration and invasion were measured by Transwell assays. Protein expression was assessed by western blot analysis. Results The expression of TRIM14 was significantly higher in CRC tissues than in matched non-cancerous tissues. TRIM14 knockdown by specific short hairpin RNA (shRNA) attenuated CRC cell migration and invasion, whereas TRIM14 overexpression caused reverse effect. Moreover, TRIM14 positively regulated the protein levels of sphingosine kinase 1 (SPHK1) and phosphorylated STAT3 (p-STAT3), as well as the mRNA and protein expression of matrix metalloproteinase 2, MMP9 and vascular endothelial growth factor, which are transcriptional targets of the STAT3 signaling pathway. Importantly, the blockage of the SPHK1/STAT3 signaling pathway by SKI-II or AG490 could reverse the TRIM14-promoted CRC cell migration and invasion. Conclusions Our results reveal a critical role for TRIM14 in promoting migration and invasion of CRC cells, and suggest TRIM14 may serve as a potential molecular target to prevent CRC metastasis.http://link.springer.com/article/10.1186/s12935-018-0701-1TRIM14STAT3SPHK1MigrationInvasion |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhonghai Jin Hongguang Li Xiaofei Hong Guangrong Ying Xiaofeng Lu Lilei Zhuang Shenbao Wu |
spellingShingle |
Zhonghai Jin Hongguang Li Xiaofei Hong Guangrong Ying Xiaofeng Lu Lilei Zhuang Shenbao Wu TRIM14 promotes colorectal cancer cell migration and invasion through the SPHK1/STAT3 pathway Cancer Cell International TRIM14 STAT3 SPHK1 Migration Invasion |
author_facet |
Zhonghai Jin Hongguang Li Xiaofei Hong Guangrong Ying Xiaofeng Lu Lilei Zhuang Shenbao Wu |
author_sort |
Zhonghai Jin |
title |
TRIM14 promotes colorectal cancer cell migration and invasion through the SPHK1/STAT3 pathway |
title_short |
TRIM14 promotes colorectal cancer cell migration and invasion through the SPHK1/STAT3 pathway |
title_full |
TRIM14 promotes colorectal cancer cell migration and invasion through the SPHK1/STAT3 pathway |
title_fullStr |
TRIM14 promotes colorectal cancer cell migration and invasion through the SPHK1/STAT3 pathway |
title_full_unstemmed |
TRIM14 promotes colorectal cancer cell migration and invasion through the SPHK1/STAT3 pathway |
title_sort |
trim14 promotes colorectal cancer cell migration and invasion through the sphk1/stat3 pathway |
publisher |
BMC |
series |
Cancer Cell International |
issn |
1475-2867 |
publishDate |
2018-12-01 |
description |
Abstract Background Colorectal cancer (CRC) is one of the most lethal malignancies. Tripartite Motif Containing 14 (TRIM14) is a member of TRIM family proteins, which are involved in the pathogenesis of various cancers. This study aimed to investigate TRIM14 expression in CRC tissues, and its effects on the migration and invasion of CRC cell lines. Methods TRIM14 mRNA expression was detected by real-time PCR analysis. Cell migration and invasion were measured by Transwell assays. Protein expression was assessed by western blot analysis. Results The expression of TRIM14 was significantly higher in CRC tissues than in matched non-cancerous tissues. TRIM14 knockdown by specific short hairpin RNA (shRNA) attenuated CRC cell migration and invasion, whereas TRIM14 overexpression caused reverse effect. Moreover, TRIM14 positively regulated the protein levels of sphingosine kinase 1 (SPHK1) and phosphorylated STAT3 (p-STAT3), as well as the mRNA and protein expression of matrix metalloproteinase 2, MMP9 and vascular endothelial growth factor, which are transcriptional targets of the STAT3 signaling pathway. Importantly, the blockage of the SPHK1/STAT3 signaling pathway by SKI-II or AG490 could reverse the TRIM14-promoted CRC cell migration and invasion. Conclusions Our results reveal a critical role for TRIM14 in promoting migration and invasion of CRC cells, and suggest TRIM14 may serve as a potential molecular target to prevent CRC metastasis. |
topic |
TRIM14 STAT3 SPHK1 Migration Invasion |
url |
http://link.springer.com/article/10.1186/s12935-018-0701-1 |
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