Post-synaptic Release of the Neuronal Tissue-Type Plasminogen Activator (tPA)
The neuronal serine protease tissue-type Plasminogen Activator (tPA) is an important player of the neuronal survival and of the synaptic plasticity. Thus, a better understanding the mechanisms regulating the neuronal trafficking of tPA is required to further understand how tPA can influence brain fu...
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doaj-4320c44fa0e744c8ab01cf323c88f93f2020-11-24T22:15:09ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022019-04-011310.3389/fncel.2019.00164451111Post-synaptic Release of the Neuronal Tissue-Type Plasminogen Activator (tPA)Sophie Lenoir0Alexandre Varangot1Laurent Lebouvier2Thierry Galli3Yannick Hommet4Denis Vivien5Denis Vivien6Physiopathology and Imaging of Neurological Disorders, UNICAEN, INSERM, UMR-S U1237, Normandie Université, Caen, FrancePhysiopathology and Imaging of Neurological Disorders, UNICAEN, INSERM, UMR-S U1237, Normandie Université, Caen, FrancePhysiopathology and Imaging of Neurological Disorders, UNICAEN, INSERM, UMR-S U1237, Normandie Université, Caen, FranceMembrane Traffic in Healthy & Diseased Brain, Center of Psychiatry and Neurosciences, INSERM U894, Sorbonne Paris-Cité, Université Paris Descartes, Paris, FrancePhysiopathology and Imaging of Neurological Disorders, UNICAEN, INSERM, UMR-S U1237, Normandie Université, Caen, FrancePhysiopathology and Imaging of Neurological Disorders, UNICAEN, INSERM, UMR-S U1237, Normandie Université, Caen, FranceDepartment of Clinical Research, Caen University Hospital, CHU Caen, Caen, FranceThe neuronal serine protease tissue-type Plasminogen Activator (tPA) is an important player of the neuronal survival and of the synaptic plasticity. Thus, a better understanding the mechanisms regulating the neuronal trafficking of tPA is required to further understand how tPA can influence brain functions. Using confocal imaging including living cells and high-resolution cell imaging combined with an innovating labeling of tPA, we demonstrate that the neuronal tPA is contained in endosomal vesicles positives for Rabs and in exosomal vesicles positives for synaptobrevin-2 (VAMP2) in dendrites and axons. tPA-containing vesicles differ in their dynamics with the dendritic tPA containing-vesicles less mobile than the axonal tPA-containing vesicles, these laters displaying mainly a retrograde trafficking. Interestingly spontaneous exocytosis of tPA containing-vesicles occurs largely in dendrites.https://www.frontiersin.org/article/10.3389/fncel.2019.00164/fulltissue-type plasminogen activatortraffickingvesiclessynapsesneurons |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sophie Lenoir Alexandre Varangot Laurent Lebouvier Thierry Galli Yannick Hommet Denis Vivien Denis Vivien |
spellingShingle |
Sophie Lenoir Alexandre Varangot Laurent Lebouvier Thierry Galli Yannick Hommet Denis Vivien Denis Vivien Post-synaptic Release of the Neuronal Tissue-Type Plasminogen Activator (tPA) Frontiers in Cellular Neuroscience tissue-type plasminogen activator trafficking vesicles synapses neurons |
author_facet |
Sophie Lenoir Alexandre Varangot Laurent Lebouvier Thierry Galli Yannick Hommet Denis Vivien Denis Vivien |
author_sort |
Sophie Lenoir |
title |
Post-synaptic Release of the Neuronal Tissue-Type Plasminogen Activator (tPA) |
title_short |
Post-synaptic Release of the Neuronal Tissue-Type Plasminogen Activator (tPA) |
title_full |
Post-synaptic Release of the Neuronal Tissue-Type Plasminogen Activator (tPA) |
title_fullStr |
Post-synaptic Release of the Neuronal Tissue-Type Plasminogen Activator (tPA) |
title_full_unstemmed |
Post-synaptic Release of the Neuronal Tissue-Type Plasminogen Activator (tPA) |
title_sort |
post-synaptic release of the neuronal tissue-type plasminogen activator (tpa) |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular Neuroscience |
issn |
1662-5102 |
publishDate |
2019-04-01 |
description |
The neuronal serine protease tissue-type Plasminogen Activator (tPA) is an important player of the neuronal survival and of the synaptic plasticity. Thus, a better understanding the mechanisms regulating the neuronal trafficking of tPA is required to further understand how tPA can influence brain functions. Using confocal imaging including living cells and high-resolution cell imaging combined with an innovating labeling of tPA, we demonstrate that the neuronal tPA is contained in endosomal vesicles positives for Rabs and in exosomal vesicles positives for synaptobrevin-2 (VAMP2) in dendrites and axons. tPA-containing vesicles differ in their dynamics with the dendritic tPA containing-vesicles less mobile than the axonal tPA-containing vesicles, these laters displaying mainly a retrograde trafficking. Interestingly spontaneous exocytosis of tPA containing-vesicles occurs largely in dendrites. |
topic |
tissue-type plasminogen activator trafficking vesicles synapses neurons |
url |
https://www.frontiersin.org/article/10.3389/fncel.2019.00164/full |
work_keys_str_mv |
AT sophielenoir postsynapticreleaseoftheneuronaltissuetypeplasminogenactivatortpa AT alexandrevarangot postsynapticreleaseoftheneuronaltissuetypeplasminogenactivatortpa AT laurentlebouvier postsynapticreleaseoftheneuronaltissuetypeplasminogenactivatortpa AT thierrygalli postsynapticreleaseoftheneuronaltissuetypeplasminogenactivatortpa AT yannickhommet postsynapticreleaseoftheneuronaltissuetypeplasminogenactivatortpa AT denisvivien postsynapticreleaseoftheneuronaltissuetypeplasminogenactivatortpa AT denisvivien postsynapticreleaseoftheneuronaltissuetypeplasminogenactivatortpa |
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