Generation of hyperlipidemic rabbit models using multiple sgRNAs targeted CRISPR/Cas9 gene editing system

Generation of hyperlipidemic rabbit models using multiple sgRNAs targeted CRISPR/Cas9 gene editing system

Abstract Objective To generate novel rabbit models with a large-fragment deletion of either LDL receptor (LDLR) and/or apolipoprotein (apoE) genes for the study of hyperlipidemic and atherosclerosis. Methods CRISPR/Cas9 system directed by a multiple sgRNAs system was used in rabbit embryos to edit t...

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Bibliographic Details
Main Authors: Tingting Yuan, Yi Zhong, Yingge Wang, Ting Zhang, Rui Lu, Minya Zhou, Yaoyao Lu, Kunning Yan, Yajie Chen, Zhehui Hu, Jingyan Liang, Jianglin Fan, Yong Cheng
Format: Article
Language:English
Published: BMC 2019-03-01
Series:Lipids in Health and Disease
Subjects:
CRISPR/Cas9
Hypercholesterolemia
Atherosclerosis
Rabbits
Multiple sgRNAs
Online Access:http://link.springer.com/article/10.1186/s12944-019-1013-8

Internet

http://link.springer.com/article/10.1186/s12944-019-1013-8

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