High-resolution monitoring from birth to sexual maturity of a male reef manta ray, Mobula alfredi, held in captivity for 7 years: changes in external morphology, behavior, and steroid hormones levels

• Main Authors: Ryo Nozu, Kiyomi Murakumo, Rui Matsumoto, Yosuke Matsumoto, Nagisa Yano, Masaru Nakamura, Makio Yanagisawa, Keiichi Ueda, Keiichi Sato
• Format: Article
• Language: English
• Published: BMC 2017-10-01
• Series: BMC Zoology
• Subjects: Reef manta ray; Mobula Alfredi; Male; Sexual maturation; In-captivity; Mating behaviors
• Online Access: http://link.springer.com/article/10.1186/s40850-017-0023-0

Abstract Background In this study, we report the first case of a male reef manta ray, Mobula alfredi, becoming sexually mature in captivity and present its reproductive characteristics. Methods We investigated changes in external morphology, behavior, and levels of steroid hormones in a male M. alfredi during its sexual maturation process. Results At 2 years and 6 months of age, the male exceeded 300 cm in disc width. Then, at around 3 years of age, the male started to chase a female in the tank and exhibited androgen levels similar to that of another matured male, indicating that the study specimen had begun sexual maturation endocrinologically. Its first copulation event was observed at 5 years and 4 months and appeared behaviorally similar to field observations. Seven months after copulation, we performed a biopsy to collect its semen, including any motile sperm. Conclusion Taken together, these results indicate that the captive male M. alfredi individual shows signs of sexual maturation (size of disc width, testosterone levels, reproductive behavior) already with the age of 2.5 to 3 years. As a first copulation was observed with 5 years 4 months of age and the presence of sperm were confirmed at the age of 5 years 11 months of age, the studied animal reached its full sexual maturity at the latest at the age of around 5 years.