In vitro induction and differentiation of umbilical cord mesenchymal stem cells into neuron-like cells by all-trans retinoic acid
AIM: To determine the optimal concentration for inducing the differentiation of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) into neuron-like cells, although it is understood that all-trans retinoic acid (ATRA) regulates cell proliferation in the nervous system by modulating the ba...
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doaj-435523b860f640bbbaba0acf5bebf0652020-11-24T22:41:39ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982015-04-018225025610.3980/j.issn.2222-3959.2015.02.07In vitro induction and differentiation of umbilical cord mesenchymal stem cells into neuron-like cells by all-trans retinoic acidWei Jin0Yao-Peng Xu1An-Huai Yang2 Yi-Qiao Xing3Eye Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, ChinaDepartment of Urology, Wuhan General Hospital of Guangzhou Military Command, Wuhan 430072, Hubei Province, China Eye Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, ChinaEye Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, ChinaAIM: To determine the optimal concentration for inducing the differentiation of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) into neuron-like cells, although it is understood that all-trans retinoic acid (ATRA) regulates cell proliferation in the nervous system by modulating the balance between mitosis and apoptosis. METHODS: The abilities of ATRA to promote apoptosis as well as neural differentiation were assessed in cultured hUC-MSCs by morphological observation, MTT assay, annexin V-FITC/PI flow cytometry and immunocytochemistry. RESULTS: The data showed that low concentrations of ATRA (0.5 µmol, 0.25 µmol) had no effect on the number of cells. However, treatment with 1.0 µmol or 2.0 µmol ATRA induced a 24.16% and 52.67% reduction in cell number, respectively, compared with vehicle-treated cultures. Further, 4.0 µmol ATRA had a potent effect on cell number, with almost no adherent cells recovered after 24h. We further showed that 0.5 µmol ATRA caused these cells to express characteristic markers of neuronal progenitor cells. CONCLUSION: Taken together, we conclude that ATRA has a dose-dependent influence on the neural differentiation and apoptosis of hUC-MSCs. These findings have implications on the use of ATRA-differentiated hUC-MSCs for the study of neural degeneration diseases. http://www.ijo.cn/en_publish/2015/2/20150207.pdfhuman umbilical cord mesenchymal stem cellsdifferentiationapoptosisneuron-like cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wei Jin Yao-Peng Xu An-Huai Yang Yi-Qiao Xing |
spellingShingle |
Wei Jin Yao-Peng Xu An-Huai Yang Yi-Qiao Xing In vitro induction and differentiation of umbilical cord mesenchymal stem cells into neuron-like cells by all-trans retinoic acid International Journal of Ophthalmology human umbilical cord mesenchymal stem cells differentiation apoptosis neuron-like cells |
author_facet |
Wei Jin Yao-Peng Xu An-Huai Yang Yi-Qiao Xing |
author_sort |
Wei Jin |
title |
In vitro induction and differentiation of umbilical cord mesenchymal stem cells into neuron-like cells by all-trans retinoic acid |
title_short |
In vitro induction and differentiation of umbilical cord mesenchymal stem cells into neuron-like cells by all-trans retinoic acid |
title_full |
In vitro induction and differentiation of umbilical cord mesenchymal stem cells into neuron-like cells by all-trans retinoic acid |
title_fullStr |
In vitro induction and differentiation of umbilical cord mesenchymal stem cells into neuron-like cells by all-trans retinoic acid |
title_full_unstemmed |
In vitro induction and differentiation of umbilical cord mesenchymal stem cells into neuron-like cells by all-trans retinoic acid |
title_sort |
in vitro induction and differentiation of umbilical cord mesenchymal stem cells into neuron-like cells by all-trans retinoic acid |
publisher |
Press of International Journal of Ophthalmology (IJO PRESS) |
series |
International Journal of Ophthalmology |
issn |
2222-3959 2227-4898 |
publishDate |
2015-04-01 |
description |
AIM: To determine the optimal concentration for inducing the differentiation of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) into neuron-like cells, although it is understood that all-trans retinoic acid (ATRA) regulates cell proliferation in the nervous system by modulating the balance between mitosis and apoptosis.
METHODS: The abilities of ATRA to promote apoptosis as well as neural differentiation were assessed in cultured hUC-MSCs by morphological observation, MTT assay, annexin V-FITC/PI flow cytometry and immunocytochemistry.
RESULTS: The data showed that low concentrations of ATRA (0.5 µmol, 0.25 µmol) had no effect on the number of cells. However, treatment with 1.0 µmol or 2.0 µmol ATRA induced a 24.16% and 52.67% reduction in cell number, respectively, compared with vehicle-treated cultures. Further, 4.0 µmol ATRA had a potent effect on cell number, with almost no adherent cells recovered after 24h. We further showed that 0.5 µmol ATRA caused these cells to express characteristic markers of neuronal progenitor cells.
CONCLUSION: Taken together, we conclude that ATRA has a dose-dependent influence on the neural differentiation and apoptosis of hUC-MSCs. These findings have implications on the use of ATRA-differentiated hUC-MSCs for the study of neural degeneration diseases.
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topic |
human umbilical cord mesenchymal stem cells differentiation apoptosis neuron-like cells |
url |
http://www.ijo.cn/en_publish/2015/2/20150207.pdf |
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