Remodeling of the Actin Network Associated with the Non-Structural Protein 1 (NS1) of West Nile Virus and Formation of NS1-Containing Tunneling Nanotubes
The cellular response to the recombinant NS1 protein of West Nile virus (NS1<sup>WNV</sup>) was studied using three different cell types: Vero E6 simian epithelial cells, SH-SY5Y human neuroblastoma cells, and U-87MG human astrocytoma cells. Cells were exposed to two different forms of N...
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doaj-437187ca8d7a4a7fb59170c14c937ed92020-11-25T01:48:11ZengMDPI AGViruses1999-49152019-09-01111090110.3390/v11100901v11100901Remodeling of the Actin Network Associated with the Non-Structural Protein 1 (NS1) of West Nile Virus and Formation of NS1-Containing Tunneling NanotubesWilhelm Furnon0Pascal Fender1Marie-Pierre Confort2Sophie Desloire3Sawitree Nangola4Kuntida Kitidee5Caroline Leroux6Maxime Ratinier7Frédérick Arnaud8Sylvie Lecollinet9Pierre Boulanger10Saw-See Hong11Université de Lyon, University Claude Bernard Lyon 1, INRA, EPHE, IVPC, UMR754, Viral Infections & Comparative Pathology, Cedex 07, 69366 Lyon, FranceInstitut de Biologie Structurale, CNRS UMR 5075, 38042 Grenoble, FranceUniversité de Lyon, University Claude Bernard Lyon 1, INRA, EPHE, IVPC, UMR754, Viral Infections & Comparative Pathology, Cedex 07, 69366 Lyon, FranceUniversité de Lyon, University Claude Bernard Lyon 1, INRA, EPHE, IVPC, UMR754, Viral Infections & Comparative Pathology, Cedex 07, 69366 Lyon, FranceDepartment of Medical Technology, School of Allied Health Sciences, University of Phayao, Phayao 56000, ThailandCenter for Research & Innovation, Faculty of Medical Technology, Mahidol University, Nakhon Pathom 73170, ThailandUniversité de Lyon, University Claude Bernard Lyon 1, INRA, EPHE, IVPC, UMR754, Viral Infections & Comparative Pathology, Cedex 07, 69366 Lyon, FranceUniversité de Lyon, University Claude Bernard Lyon 1, INRA, EPHE, IVPC, UMR754, Viral Infections & Comparative Pathology, Cedex 07, 69366 Lyon, FranceUniversité de Lyon, University Claude Bernard Lyon 1, INRA, EPHE, IVPC, UMR754, Viral Infections & Comparative Pathology, Cedex 07, 69366 Lyon, FranceUMR-1161 Virology, ANSES, INRA, Ecole Nationale Vétérinaire d’Alfort, ANSES Animal Health Laboratory, EURL on Equine Diseases, 94704 Maisons-Alfort, FranceUniversité de Lyon, University Claude Bernard Lyon 1, INRA, EPHE, IVPC, UMR754, Viral Infections & Comparative Pathology, Cedex 07, 69366 Lyon, FranceUniversité de Lyon, University Claude Bernard Lyon 1, INRA, EPHE, IVPC, UMR754, Viral Infections & Comparative Pathology, Cedex 07, 69366 Lyon, FranceThe cellular response to the recombinant NS1 protein of West Nile virus (NS1<sup>WNV</sup>) was studied using three different cell types: Vero E6 simian epithelial cells, SH-SY5Y human neuroblastoma cells, and U-87MG human astrocytoma cells. Cells were exposed to two different forms of NS1<sup>WNV</sup>: (i) the exogenous secreted form, sNS1<sup>WNV</sup>, added to the extracellular milieu; and (ii) the endogenous NS1<sup>WNV</sup>, the intracellular form expressed in plasmid-transfected cells. The cell attachment and uptake of sNS1<sup>WNV</sup> varied with the cell type and were only detectable in Vero E6 and SH-SY5Y cells. Addition of sNS1<sup>WNV</sup> to the cell culture medium resulted in significant remodeling of the actin filament network in Vero E6 cells. This effect was not observed in SH-SY5Y and U-87MG cells, implying that the cellular uptake of sNS1<sup>WNV</sup> and actin network remodeling were dependent on cell type. In the three cell types, NS1<sup>WNV</sup>-expressing cells formed filamentous projections reminiscent of tunneling nanotubes (TNTs). These TNT-like projections were found to contain actin and NS1<sup>WNV</sup> proteins. Interestingly, similar actin-rich, TNT-like filaments containing NS1<sup>WNV</sup> and the viral envelope glycoprotein E<sup>WNV</sup> were also observed in WNV-infected Vero E6 cells.https://www.mdpi.com/1999-4915/11/10/901flaviviruswest nile virusnon-structural protein 1 (ns1)cytoskeletonactin networktunneling nanotubes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wilhelm Furnon Pascal Fender Marie-Pierre Confort Sophie Desloire Sawitree Nangola Kuntida Kitidee Caroline Leroux Maxime Ratinier Frédérick Arnaud Sylvie Lecollinet Pierre Boulanger Saw-See Hong |
spellingShingle |
Wilhelm Furnon Pascal Fender Marie-Pierre Confort Sophie Desloire Sawitree Nangola Kuntida Kitidee Caroline Leroux Maxime Ratinier Frédérick Arnaud Sylvie Lecollinet Pierre Boulanger Saw-See Hong Remodeling of the Actin Network Associated with the Non-Structural Protein 1 (NS1) of West Nile Virus and Formation of NS1-Containing Tunneling Nanotubes Viruses flavivirus west nile virus non-structural protein 1 (ns1) cytoskeleton actin network tunneling nanotubes |
author_facet |
Wilhelm Furnon Pascal Fender Marie-Pierre Confort Sophie Desloire Sawitree Nangola Kuntida Kitidee Caroline Leroux Maxime Ratinier Frédérick Arnaud Sylvie Lecollinet Pierre Boulanger Saw-See Hong |
author_sort |
Wilhelm Furnon |
title |
Remodeling of the Actin Network Associated with the Non-Structural Protein 1 (NS1) of West Nile Virus and Formation of NS1-Containing Tunneling Nanotubes |
title_short |
Remodeling of the Actin Network Associated with the Non-Structural Protein 1 (NS1) of West Nile Virus and Formation of NS1-Containing Tunneling Nanotubes |
title_full |
Remodeling of the Actin Network Associated with the Non-Structural Protein 1 (NS1) of West Nile Virus and Formation of NS1-Containing Tunneling Nanotubes |
title_fullStr |
Remodeling of the Actin Network Associated with the Non-Structural Protein 1 (NS1) of West Nile Virus and Formation of NS1-Containing Tunneling Nanotubes |
title_full_unstemmed |
Remodeling of the Actin Network Associated with the Non-Structural Protein 1 (NS1) of West Nile Virus and Formation of NS1-Containing Tunneling Nanotubes |
title_sort |
remodeling of the actin network associated with the non-structural protein 1 (ns1) of west nile virus and formation of ns1-containing tunneling nanotubes |
publisher |
MDPI AG |
series |
Viruses |
issn |
1999-4915 |
publishDate |
2019-09-01 |
description |
The cellular response to the recombinant NS1 protein of West Nile virus (NS1<sup>WNV</sup>) was studied using three different cell types: Vero E6 simian epithelial cells, SH-SY5Y human neuroblastoma cells, and U-87MG human astrocytoma cells. Cells were exposed to two different forms of NS1<sup>WNV</sup>: (i) the exogenous secreted form, sNS1<sup>WNV</sup>, added to the extracellular milieu; and (ii) the endogenous NS1<sup>WNV</sup>, the intracellular form expressed in plasmid-transfected cells. The cell attachment and uptake of sNS1<sup>WNV</sup> varied with the cell type and were only detectable in Vero E6 and SH-SY5Y cells. Addition of sNS1<sup>WNV</sup> to the cell culture medium resulted in significant remodeling of the actin filament network in Vero E6 cells. This effect was not observed in SH-SY5Y and U-87MG cells, implying that the cellular uptake of sNS1<sup>WNV</sup> and actin network remodeling were dependent on cell type. In the three cell types, NS1<sup>WNV</sup>-expressing cells formed filamentous projections reminiscent of tunneling nanotubes (TNTs). These TNT-like projections were found to contain actin and NS1<sup>WNV</sup> proteins. Interestingly, similar actin-rich, TNT-like filaments containing NS1<sup>WNV</sup> and the viral envelope glycoprotein E<sup>WNV</sup> were also observed in WNV-infected Vero E6 cells. |
topic |
flavivirus west nile virus non-structural protein 1 (ns1) cytoskeleton actin network tunneling nanotubes |
url |
https://www.mdpi.com/1999-4915/11/10/901 |
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