Diversity of Mycobacterium tuberculosis Isolates from New Pulmonary Tuberculosis Cases in Addis Ababa, Ethiopia
Understanding the genetic diversity of Mycobacterium tuberculosis is needed for a better understanding of the epidemiology of TB and could have implications for the development of new diagnostics, drugs, and vaccines. M. tuberculosis isolates were characterized using spoligotyping and were compared...
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doaj-438ad6225b8a4b789ae9c2cfe248d2732020-11-24T23:13:55ZengHindawi LimitedTuberculosis Research and Treatment2090-150X2090-15182012-01-01201210.1155/2012/892079892079Diversity of Mycobacterium tuberculosis Isolates from New Pulmonary Tuberculosis Cases in Addis Ababa, EthiopiaAdane Mihret0Yonas Bekele1Andre G. Loxton2Annemie M. Jordan3Lawrence Yamuah4Abraham Aseffa5Rawleigh Howe6Gerhard Walzl7Immunology Unit, Armauer Hansen Research Institute, P.O. Box 1005, Addis Ababa, EthiopiaImmunology Unit, Armauer Hansen Research Institute, P.O. Box 1005, Addis Ababa, EthiopiaDivision of Molecular Biology and Human Genetics, DST/NRF Centre of Excellence for Biomedical Tuberculosis Research, MRC Centre for Molecular and Cellular Biology, Faculty of Medicine and Health Sciences, Stellenbosch University, P.O. Box 19063, Francie van Zijl Drive, Tygerberg 7505, South AfricaDivision of Molecular Biology and Human Genetics, DST/NRF Centre of Excellence for Biomedical Tuberculosis Research, MRC Centre for Molecular and Cellular Biology, Faculty of Medicine and Health Sciences, Stellenbosch University, P.O. Box 19063, Francie van Zijl Drive, Tygerberg 7505, South AfricaImmunology Unit, Armauer Hansen Research Institute, P.O. Box 1005, Addis Ababa, EthiopiaImmunology Unit, Armauer Hansen Research Institute, P.O. Box 1005, Addis Ababa, EthiopiaImmunology Unit, Armauer Hansen Research Institute, P.O. Box 1005, Addis Ababa, EthiopiaDivision of Molecular Biology and Human Genetics, DST/NRF Centre of Excellence for Biomedical Tuberculosis Research, MRC Centre for Molecular and Cellular Biology, Faculty of Medicine and Health Sciences, Stellenbosch University, P.O. Box 19063, Francie van Zijl Drive, Tygerberg 7505, South AfricaUnderstanding the genetic diversity of Mycobacterium tuberculosis is needed for a better understanding of the epidemiology of TB and could have implications for the development of new diagnostics, drugs, and vaccines. M. tuberculosis isolates were characterized using spoligotyping and were compared with the SpoIDB4 database of the Pasteur Institute of Guadeloupe. A total of 53 different patterns were identified among 192 isolates examined. 169 of the isolates were classified into one of the 33 shared SITs, whereas the remaining 23 corresponded to 20 orphan patterns. 54% of the isolates were ascribed to the T family, a family which has not been well defined to date. Other prominent families were CAS, Haarlem, LAM, Beijing, and Unknown comprising 26%, 13%, 2.6%, 0.5%, and 2.1%, respectively. Among HIV-positive patients, 10 patterns were observed among 25 isolates. The T (38.5%), H (26.9%), and CAS (23.1%) families were the most common among HIV-positive individuals. The diversity of the M. tuberculosis strains found in this study is very high, and there was no difference in the distribution of families in HIV-positive and HIV-negative TB patients except the H family. Tuberculosis transmission in Addis Ababa is due to only the modern M. tuberculosis families (CAS, LAM, T, Beijing, Haarlem, and U).http://dx.doi.org/10.1155/2012/892079 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Adane Mihret Yonas Bekele Andre G. Loxton Annemie M. Jordan Lawrence Yamuah Abraham Aseffa Rawleigh Howe Gerhard Walzl |
spellingShingle |
Adane Mihret Yonas Bekele Andre G. Loxton Annemie M. Jordan Lawrence Yamuah Abraham Aseffa Rawleigh Howe Gerhard Walzl Diversity of Mycobacterium tuberculosis Isolates from New Pulmonary Tuberculosis Cases in Addis Ababa, Ethiopia Tuberculosis Research and Treatment |
author_facet |
Adane Mihret Yonas Bekele Andre G. Loxton Annemie M. Jordan Lawrence Yamuah Abraham Aseffa Rawleigh Howe Gerhard Walzl |
author_sort |
Adane Mihret |
title |
Diversity of Mycobacterium tuberculosis Isolates from New Pulmonary Tuberculosis Cases in Addis Ababa, Ethiopia |
title_short |
Diversity of Mycobacterium tuberculosis Isolates from New Pulmonary Tuberculosis Cases in Addis Ababa, Ethiopia |
title_full |
Diversity of Mycobacterium tuberculosis Isolates from New Pulmonary Tuberculosis Cases in Addis Ababa, Ethiopia |
title_fullStr |
Diversity of Mycobacterium tuberculosis Isolates from New Pulmonary Tuberculosis Cases in Addis Ababa, Ethiopia |
title_full_unstemmed |
Diversity of Mycobacterium tuberculosis Isolates from New Pulmonary Tuberculosis Cases in Addis Ababa, Ethiopia |
title_sort |
diversity of mycobacterium tuberculosis isolates from new pulmonary tuberculosis cases in addis ababa, ethiopia |
publisher |
Hindawi Limited |
series |
Tuberculosis Research and Treatment |
issn |
2090-150X 2090-1518 |
publishDate |
2012-01-01 |
description |
Understanding the genetic diversity of Mycobacterium tuberculosis is needed for a better understanding of the epidemiology of TB and could have implications for the development of new diagnostics, drugs, and vaccines. M. tuberculosis isolates were characterized using spoligotyping and were compared with the SpoIDB4 database of the Pasteur Institute of Guadeloupe. A total of 53 different patterns were identified among 192 isolates examined. 169 of the isolates were classified into one of the 33 shared SITs, whereas the remaining 23 corresponded to 20 orphan patterns. 54% of the isolates were ascribed to the T family, a family which has not been well defined to date. Other prominent families were CAS, Haarlem, LAM, Beijing, and Unknown comprising 26%, 13%, 2.6%, 0.5%, and 2.1%, respectively. Among HIV-positive patients, 10 patterns were observed among 25 isolates. The T (38.5%), H (26.9%), and CAS (23.1%) families were the most common among HIV-positive individuals. The diversity of the M. tuberculosis strains found in this study is very high, and there was no difference in the distribution of families in HIV-positive and HIV-negative TB patients except the H family. Tuberculosis transmission in Addis Ababa is due to only the modern M. tuberculosis families (CAS, LAM, T, Beijing, Haarlem, and U). |
url |
http://dx.doi.org/10.1155/2012/892079 |
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