Genes <i>ScBx1</i> and <i>ScIgl</i>—Competitors or Cooperators?
Two genes, <i>Bx1</i> and <i>Igl</i>, both encoding indole-3-glycerol phosphate lyase (IGL), are believed to control the conversion of indole-3-glycerol phosphate (IGP) to indole. The first of these has generally been supposed to be regulated developmentally, being expressed...
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doaj-43966a642d30443e90cbaf2ed7dc44ba2020-11-25T00:31:11ZengMDPI AGGenes2073-44252020-02-0111222310.3390/genes11020223genes11020223Genes <i>ScBx1</i> and <i>ScIgl</i>—Competitors or Cooperators?Anna Wlazło0Magdalena Święcicka1Marek D. Koter2Tomasz Krępski3Leszek Bolibok4Anna Stochmal5Mariusz Kowalczyk6Monika Rakoczy-Trojanowska7Department of Plant Genetics, Breeding and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-787 Warsaw, PolandDepartment of Plant Genetics, Breeding and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-787 Warsaw, PolandDepartment of Plant Genetics, Breeding and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-787 Warsaw, PolandDepartment of Plant Genetics, Breeding and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-787 Warsaw, PolandDepartment of Silviculture, Institute of Forest Sciences, Warsaw University of Life Sciences, 02-787 Warsaw, PolandDepartment of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100 Puławy, PolandDepartment of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100 Puławy, PolandDepartment of Plant Genetics, Breeding and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-787 Warsaw, PolandTwo genes, <i>Bx1</i> and <i>Igl</i>, both encoding indole-3-glycerol phosphate lyase (IGL), are believed to control the conversion of indole-3-glycerol phosphate (IGP) to indole. The first of these has generally been supposed to be regulated developmentally, being expressed at early stages of plant development with the indole being used in the benzoxazinoid (BX) biosynthesis pathway. In contrast, it has been proposed that the second one is regulated by stresses and that the associated free indole is secreted as a volatile. However, our previous results contradicted this. In the present study, we show that the <i>ScIgl</i> gene takes over the role of <i>ScBx1</i> at later developmental stages, between the 42nd and 70th days after germination. In the majority of plants with silenced <i>ScBx1</i> expression, <i>ScIgl</i> was either expressed at a significantly higher level than <i>ScBx1</i> or it was the only gene with detectable expression. Therefore, we postulate that the synthesis of indole used in BX biosynthesis in rye is controlled by both <i>ScBx1</i> and <i>ScIgl</i>, which are both regulated developmentally and by stresses. In silico and in vivo analyses of the promoter sequences further confirmed our hypothesis that the roles and modes of regulation of the <i>ScBx1</i> and <i>ScIgl</i> genes are similar.https://www.mdpi.com/2073-4425/11/2/223ryegene expressiongene silencingbenzoxazinoid biosynthesis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anna Wlazło Magdalena Święcicka Marek D. Koter Tomasz Krępski Leszek Bolibok Anna Stochmal Mariusz Kowalczyk Monika Rakoczy-Trojanowska |
spellingShingle |
Anna Wlazło Magdalena Święcicka Marek D. Koter Tomasz Krępski Leszek Bolibok Anna Stochmal Mariusz Kowalczyk Monika Rakoczy-Trojanowska Genes <i>ScBx1</i> and <i>ScIgl</i>—Competitors or Cooperators? Genes rye gene expression gene silencing benzoxazinoid biosynthesis |
author_facet |
Anna Wlazło Magdalena Święcicka Marek D. Koter Tomasz Krępski Leszek Bolibok Anna Stochmal Mariusz Kowalczyk Monika Rakoczy-Trojanowska |
author_sort |
Anna Wlazło |
title |
Genes <i>ScBx1</i> and <i>ScIgl</i>—Competitors or Cooperators? |
title_short |
Genes <i>ScBx1</i> and <i>ScIgl</i>—Competitors or Cooperators? |
title_full |
Genes <i>ScBx1</i> and <i>ScIgl</i>—Competitors or Cooperators? |
title_fullStr |
Genes <i>ScBx1</i> and <i>ScIgl</i>—Competitors or Cooperators? |
title_full_unstemmed |
Genes <i>ScBx1</i> and <i>ScIgl</i>—Competitors or Cooperators? |
title_sort |
genes <i>scbx1</i> and <i>scigl</i>—competitors or cooperators? |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2020-02-01 |
description |
Two genes, <i>Bx1</i> and <i>Igl</i>, both encoding indole-3-glycerol phosphate lyase (IGL), are believed to control the conversion of indole-3-glycerol phosphate (IGP) to indole. The first of these has generally been supposed to be regulated developmentally, being expressed at early stages of plant development with the indole being used in the benzoxazinoid (BX) biosynthesis pathway. In contrast, it has been proposed that the second one is regulated by stresses and that the associated free indole is secreted as a volatile. However, our previous results contradicted this. In the present study, we show that the <i>ScIgl</i> gene takes over the role of <i>ScBx1</i> at later developmental stages, between the 42nd and 70th days after germination. In the majority of plants with silenced <i>ScBx1</i> expression, <i>ScIgl</i> was either expressed at a significantly higher level than <i>ScBx1</i> or it was the only gene with detectable expression. Therefore, we postulate that the synthesis of indole used in BX biosynthesis in rye is controlled by both <i>ScBx1</i> and <i>ScIgl</i>, which are both regulated developmentally and by stresses. In silico and in vivo analyses of the promoter sequences further confirmed our hypothesis that the roles and modes of regulation of the <i>ScBx1</i> and <i>ScIgl</i> genes are similar. |
topic |
rye gene expression gene silencing benzoxazinoid biosynthesis |
url |
https://www.mdpi.com/2073-4425/11/2/223 |
work_keys_str_mv |
AT annawlazło genesiscbx1iandisciglicompetitorsorcooperators AT magdalenaswiecicka genesiscbx1iandisciglicompetitorsorcooperators AT marekdkoter genesiscbx1iandisciglicompetitorsorcooperators AT tomaszkrepski genesiscbx1iandisciglicompetitorsorcooperators AT leszekbolibok genesiscbx1iandisciglicompetitorsorcooperators AT annastochmal genesiscbx1iandisciglicompetitorsorcooperators AT mariuszkowalczyk genesiscbx1iandisciglicompetitorsorcooperators AT monikarakoczytrojanowska genesiscbx1iandisciglicompetitorsorcooperators |
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