Proteogenomic Characterization of Novel x-Type High Molecular Weight Glutenin Subunit 1Ax1.1

Analysis of Portuguese wheat (Triticum aestivum L.) landrace ‘Barbela’ revealed the existence of a new x-type high molecular weight-glutenin subunit (HMW-GS) encoded at the Glu-A1 locus, which we named 1Ax1.1. Using one-dimensional and two-dimensional electrophoresis and mass spectrometry, we compar...

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Main Authors: Gilberto Igrejas, Gérard Branlard, Catherine Ravel, Mireille Dardevet, Annie Faye, Emmanuelle Bancel, Miguel Ribeiro
Format: Article
Language:English
Published: MDPI AG 2013-03-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:http://www.mdpi.com/1422-0067/14/3/5650
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spelling doaj-43b32e091be14f19abf1b6d555df92c72020-11-25T00:29:57ZengMDPI AGInternational Journal of Molecular Sciences1422-00672013-03-011435650566710.3390/ijms14035650Proteogenomic Characterization of Novel x-Type High Molecular Weight Glutenin Subunit 1Ax1.1Gilberto IgrejasGérard BranlardCatherine RavelMireille DardevetAnnie FayeEmmanuelle BancelMiguel RibeiroAnalysis of Portuguese wheat (Triticum aestivum L.) landrace ‘Barbela’ revealed the existence of a new x-type high molecular weight-glutenin subunit (HMW-GS) encoded at the Glu-A1 locus, which we named 1Ax1.1. Using one-dimensional and two-dimensional electrophoresis and mass spectrometry, we compared subunit 1Ax1.1 with other subunits encoded at the Glu-A1 locus. Subunit 1Ax1.1 has a theoretical molecular weight of 93,648 Da (or 91,508 Da for the mature protein) and an isoelectric point (pI) of about 5.7, making it the largest and most acidic HMW-GS known to be encoded at Glu-A1. Specific primers were designed to amplify and sequence 2601 bp of the Glu-A1 locus from the ‘Barbela 28’ wheat genome. A very high level of identity was found between the sequence encoding 1Ax1.1 and those encoding other alleles of the locus. The major difference found was an insertion of 36 amino acids in the central repetitive domain.http://www.mdpi.com/1422-0067/14/3/5650Triticum aestivum L.wheatstorage proteinsgluteninsHMW-GSGlu-A11Ax1.1MALDI-TOF-MS
collection DOAJ
language English
format Article
sources DOAJ
author Gilberto Igrejas
Gérard Branlard
Catherine Ravel
Mireille Dardevet
Annie Faye
Emmanuelle Bancel
Miguel Ribeiro
spellingShingle Gilberto Igrejas
Gérard Branlard
Catherine Ravel
Mireille Dardevet
Annie Faye
Emmanuelle Bancel
Miguel Ribeiro
Proteogenomic Characterization of Novel x-Type High Molecular Weight Glutenin Subunit 1Ax1.1
International Journal of Molecular Sciences
Triticum aestivum L.
wheat
storage proteins
glutenins
HMW-GS
Glu-A1
1Ax1.1
MALDI-TOF-MS
author_facet Gilberto Igrejas
Gérard Branlard
Catherine Ravel
Mireille Dardevet
Annie Faye
Emmanuelle Bancel
Miguel Ribeiro
author_sort Gilberto Igrejas
title Proteogenomic Characterization of Novel x-Type High Molecular Weight Glutenin Subunit 1Ax1.1
title_short Proteogenomic Characterization of Novel x-Type High Molecular Weight Glutenin Subunit 1Ax1.1
title_full Proteogenomic Characterization of Novel x-Type High Molecular Weight Glutenin Subunit 1Ax1.1
title_fullStr Proteogenomic Characterization of Novel x-Type High Molecular Weight Glutenin Subunit 1Ax1.1
title_full_unstemmed Proteogenomic Characterization of Novel x-Type High Molecular Weight Glutenin Subunit 1Ax1.1
title_sort proteogenomic characterization of novel x-type high molecular weight glutenin subunit 1ax1.1
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1422-0067
publishDate 2013-03-01
description Analysis of Portuguese wheat (Triticum aestivum L.) landrace ‘Barbela’ revealed the existence of a new x-type high molecular weight-glutenin subunit (HMW-GS) encoded at the Glu-A1 locus, which we named 1Ax1.1. Using one-dimensional and two-dimensional electrophoresis and mass spectrometry, we compared subunit 1Ax1.1 with other subunits encoded at the Glu-A1 locus. Subunit 1Ax1.1 has a theoretical molecular weight of 93,648 Da (or 91,508 Da for the mature protein) and an isoelectric point (pI) of about 5.7, making it the largest and most acidic HMW-GS known to be encoded at Glu-A1. Specific primers were designed to amplify and sequence 2601 bp of the Glu-A1 locus from the ‘Barbela 28’ wheat genome. A very high level of identity was found between the sequence encoding 1Ax1.1 and those encoding other alleles of the locus. The major difference found was an insertion of 36 amino acids in the central repetitive domain.
topic Triticum aestivum L.
wheat
storage proteins
glutenins
HMW-GS
Glu-A1
1Ax1.1
MALDI-TOF-MS
url http://www.mdpi.com/1422-0067/14/3/5650
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