Recent Trends in Enzyme Immobilization—Concepts for Expanding the Biocatalysis Toolbox
Enzymes have been exploited by humans for thousands of years in brewing and baking, but it is only recently that biocatalysis has become a mainstream technology for synthesis. Today, enzymes are used extensively in the manufacturing of pharmaceuticals, food, fine chemicals, flavors, fragrances and o...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-05-01
|
Series: | Molecules |
Subjects: | |
Online Access: | https://www.mdpi.com/1420-3049/26/9/2822 |
id |
doaj-43b588e894dc43168d02cc82322c8ece |
---|---|
record_format |
Article |
spelling |
doaj-43b588e894dc43168d02cc82322c8ece2021-05-31T23:35:53ZengMDPI AGMolecules1420-30492021-05-01262822282210.3390/molecules26092822Recent Trends in Enzyme Immobilization—Concepts for Expanding the Biocatalysis ToolboxHans-Jürgen Federsel0Thomas S. Moody1Steve J.C. Taylor2RISE Research Institutes of Sweden, Department of Chemical Process and Pharmaceutical Development, P.O. Box 5607, S-114 86 Stockholm, SwedenAlmac Sciences Ltd., 20 Seagoe Industrial Estate, Craigavon BT63 5QD, UKAlmac Sciences Ltd., 20 Seagoe Industrial Estate, Craigavon BT63 5QD, UKEnzymes have been exploited by humans for thousands of years in brewing and baking, but it is only recently that biocatalysis has become a mainstream technology for synthesis. Today, enzymes are used extensively in the manufacturing of pharmaceuticals, food, fine chemicals, flavors, fragrances and other products. Enzyme immobilization technology has also developed in parallel as a means of increasing enzyme performance and reducing process costs. The aim of this review is to present and discuss some of the more recent promising technical developments in enzyme immobilization, including the supports used, methods of fabrication, and their application in synthesis. The review highlights new support technologies such as the use of well-established polysaccharides in novel ways, the use of magnetic particles, DNA, renewable materials and hybrid organic–inorganic supports. The review also addresses how immobilization is being integrated into developing biocatalytic technology, for example in flow biocatalysis, the use of 3D printing and multi-enzymatic cascade reactions.https://www.mdpi.com/1420-3049/26/9/2822enzymebiocatalysisimmobilizationflow processessupport technologies3D-printing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hans-Jürgen Federsel Thomas S. Moody Steve J.C. Taylor |
spellingShingle |
Hans-Jürgen Federsel Thomas S. Moody Steve J.C. Taylor Recent Trends in Enzyme Immobilization—Concepts for Expanding the Biocatalysis Toolbox Molecules enzyme biocatalysis immobilization flow processes support technologies 3D-printing |
author_facet |
Hans-Jürgen Federsel Thomas S. Moody Steve J.C. Taylor |
author_sort |
Hans-Jürgen Federsel |
title |
Recent Trends in Enzyme Immobilization—Concepts for Expanding the Biocatalysis Toolbox |
title_short |
Recent Trends in Enzyme Immobilization—Concepts for Expanding the Biocatalysis Toolbox |
title_full |
Recent Trends in Enzyme Immobilization—Concepts for Expanding the Biocatalysis Toolbox |
title_fullStr |
Recent Trends in Enzyme Immobilization—Concepts for Expanding the Biocatalysis Toolbox |
title_full_unstemmed |
Recent Trends in Enzyme Immobilization—Concepts for Expanding the Biocatalysis Toolbox |
title_sort |
recent trends in enzyme immobilization—concepts for expanding the biocatalysis toolbox |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2021-05-01 |
description |
Enzymes have been exploited by humans for thousands of years in brewing and baking, but it is only recently that biocatalysis has become a mainstream technology for synthesis. Today, enzymes are used extensively in the manufacturing of pharmaceuticals, food, fine chemicals, flavors, fragrances and other products. Enzyme immobilization technology has also developed in parallel as a means of increasing enzyme performance and reducing process costs. The aim of this review is to present and discuss some of the more recent promising technical developments in enzyme immobilization, including the supports used, methods of fabrication, and their application in synthesis. The review highlights new support technologies such as the use of well-established polysaccharides in novel ways, the use of magnetic particles, DNA, renewable materials and hybrid organic–inorganic supports. The review also addresses how immobilization is being integrated into developing biocatalytic technology, for example in flow biocatalysis, the use of 3D printing and multi-enzymatic cascade reactions. |
topic |
enzyme biocatalysis immobilization flow processes support technologies 3D-printing |
url |
https://www.mdpi.com/1420-3049/26/9/2822 |
work_keys_str_mv |
AT hansjurgenfedersel recenttrendsinenzymeimmobilizationconceptsforexpandingthebiocatalysistoolbox AT thomassmoody recenttrendsinenzymeimmobilizationconceptsforexpandingthebiocatalysistoolbox AT stevejctaylor recenttrendsinenzymeimmobilizationconceptsforexpandingthebiocatalysistoolbox |
_version_ |
1721417039762948096 |