Molecular Biological Study of Anti-cancer Effects of Bee Venom Aqua-acupuncture
To study anti-cancer effect and molecular biological mechanism of bee venom for aqua-acupuncture, the effects of bee venom on cell viability and apoptosis were analyzed using MTT assay, tryphan blue assay, [3H]thymidine release assay, flow cytometric analysis, and activity of caspase-3 protease acti...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Korean Pharmacopuncture Institute
2000-07-01
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| Series: | Journal of Pharmacopuncture |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3831/KPI.2000.3.1.001 |
| Summary: | To study anti-cancer effect and molecular biological mechanism of bee venom for aqua-acupuncture, the effects of bee venom on cell viability and apoptosis were analyzed using MTT assay, tryphan blue assay, [3H]thymidine release assay, flow cytometric analysis, and activity of caspase-3 protease activity assay. To explore whether anti-cancer effects of bee venom are associated with the transcriptional control of gene expression, quantitative RT-PCR analysis of apoptosis-related genes was performed.
The obtained results are summarized as follows:
1. The MTT assay demonstrated that cell viability was decreased by bee venom in a dose-dependant manner.
2. Significant induction of apoptosis was identified using tryphan blue assay, [3H]thymidine release assay, and flow cytometric analysis of sub G1 fraction.
3. In analysis of caspase-3 protease activity, the activity had increased significantly, in a dose-dependant manner.
4. Quantitative RT-PCR analysis of the apoptosis-related genes showed that Bcl-2 and Bcl-XL were down-regulated whereas Bax was up-regulated by bee venom treatment.
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| ISSN: | 2093-6966 2234-6856 |