Antioxidant and antimicrobial potential of selected varieties of Piper betle L. (Betel leaf)

Abstract Piper betle L., is an evergreen perennial creeper belonging to family Piperaceae and is known to possess numerous medicinal properties. Current study focuses on evaluating antioxidant and antimicrobial potential of betel leaf. For the present study, distilled water, hexane, acetone and etha...

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Main Authors: CHAYANIKA SARMA, PRASAD RASANE, SAWINDER KAUR, JYOTI SINGH, JOGINDER SINGH, YOGESH GAT, UMAR GARBA, DAMANPREET KAUR, KAJAL DHAWAN
Format: Article
Language:English
Published: Academia Brasileira de Ciências 2018-10-01
Series:Anais da Academia Brasileira de Ciências
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018005015118&lng=en&tlng=en
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spelling doaj-43c70d3d5d664699b3d69dfc24e61c5f2020-11-24T20:59:57ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902018-10-01010.1590/0001-3765201820180285S0001-37652018005015118Antioxidant and antimicrobial potential of selected varieties of Piper betle L. (Betel leaf)CHAYANIKA SARMAPRASAD RASANESAWINDER KAURJYOTI SINGHJOGINDER SINGHYOGESH GATUMAR GARBADAMANPREET KAURKAJAL DHAWANAbstract Piper betle L., is an evergreen perennial creeper belonging to family Piperaceae and is known to possess numerous medicinal properties. Current study focuses on evaluating antioxidant and antimicrobial potential of betel leaf. For the present study, distilled water, hexane, acetone and ethanolic extracts of two varieties of betel leaves: Meetha paan and Banarasi paan were used. Biochemical tests such as proximate analysis (moisture, ash, protein, lipids, minerals viz., sodium and potassium), antioxidant activity tests (DPPH radical scavenging activity, total phenolics, ascorbic acid, reducing power) and antimicrobial test (antibacterial and antifungal susceptibility test) against four pathogens viz., B. subtilis, E. coli, A. niger and S. cerevisiae were determined. Ethanolic extract had the highest antioxidant activity (89.46% inhibition), while the aqueous extract exhibited lowest antioxidant activity (62.03% inhibition). With increasing concentration (5, 10, 25 and 50 µg/mL), the reducing power of leaf extracts also increased. The ascorbic acid was not significant in Banarasi paan (5.21mg/100 g) and Meetha paan (5.20mg/100 g). The highest antibacterial activity of ethanolic extract (Banarasi paan) may be attributed to the presence of phytosterols in the leaf varieties. Antioxidant and antimicrobial potential study will help to build a database and promote the utilization of betel leaf as a medicinal herb.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018005015118&lng=en&tlng=enPiper betlepiperaceaeantioxidantantimicrobialbiochemical
collection DOAJ
language English
format Article
sources DOAJ
author CHAYANIKA SARMA
PRASAD RASANE
SAWINDER KAUR
JYOTI SINGH
JOGINDER SINGH
YOGESH GAT
UMAR GARBA
DAMANPREET KAUR
KAJAL DHAWAN
spellingShingle CHAYANIKA SARMA
PRASAD RASANE
SAWINDER KAUR
JYOTI SINGH
JOGINDER SINGH
YOGESH GAT
UMAR GARBA
DAMANPREET KAUR
KAJAL DHAWAN
Antioxidant and antimicrobial potential of selected varieties of Piper betle L. (Betel leaf)
Anais da Academia Brasileira de Ciências
Piper betle
piperaceae
antioxidant
antimicrobial
biochemical
author_facet CHAYANIKA SARMA
PRASAD RASANE
SAWINDER KAUR
JYOTI SINGH
JOGINDER SINGH
YOGESH GAT
UMAR GARBA
DAMANPREET KAUR
KAJAL DHAWAN
author_sort CHAYANIKA SARMA
title Antioxidant and antimicrobial potential of selected varieties of Piper betle L. (Betel leaf)
title_short Antioxidant and antimicrobial potential of selected varieties of Piper betle L. (Betel leaf)
title_full Antioxidant and antimicrobial potential of selected varieties of Piper betle L. (Betel leaf)
title_fullStr Antioxidant and antimicrobial potential of selected varieties of Piper betle L. (Betel leaf)
title_full_unstemmed Antioxidant and antimicrobial potential of selected varieties of Piper betle L. (Betel leaf)
title_sort antioxidant and antimicrobial potential of selected varieties of piper betle l. (betel leaf)
publisher Academia Brasileira de Ciências
series Anais da Academia Brasileira de Ciências
issn 1678-2690
publishDate 2018-10-01
description Abstract Piper betle L., is an evergreen perennial creeper belonging to family Piperaceae and is known to possess numerous medicinal properties. Current study focuses on evaluating antioxidant and antimicrobial potential of betel leaf. For the present study, distilled water, hexane, acetone and ethanolic extracts of two varieties of betel leaves: Meetha paan and Banarasi paan were used. Biochemical tests such as proximate analysis (moisture, ash, protein, lipids, minerals viz., sodium and potassium), antioxidant activity tests (DPPH radical scavenging activity, total phenolics, ascorbic acid, reducing power) and antimicrobial test (antibacterial and antifungal susceptibility test) against four pathogens viz., B. subtilis, E. coli, A. niger and S. cerevisiae were determined. Ethanolic extract had the highest antioxidant activity (89.46% inhibition), while the aqueous extract exhibited lowest antioxidant activity (62.03% inhibition). With increasing concentration (5, 10, 25 and 50 µg/mL), the reducing power of leaf extracts also increased. The ascorbic acid was not significant in Banarasi paan (5.21mg/100 g) and Meetha paan (5.20mg/100 g). The highest antibacterial activity of ethanolic extract (Banarasi paan) may be attributed to the presence of phytosterols in the leaf varieties. Antioxidant and antimicrobial potential study will help to build a database and promote the utilization of betel leaf as a medicinal herb.
topic Piper betle
piperaceae
antioxidant
antimicrobial
biochemical
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018005015118&lng=en&tlng=en
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