Circulating MiR-19b-3p, MiR-134-5p and MiR-186-5p are Promising Novel Biomarkers for Early Diagnosis of Acute Myocardial Infarction
Background/Aims: Recent studies have shown that circulating microRNAs (miRNAs) are emerging as promising biomarkers for cardiovascular diseases. This study aimed to determine whether miR-19b-3p, miR-134-5p and miR-186-5p can be used as novel indicators for acute myocardial infarction (AMI). Methods:...
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Cell Physiol Biochem Press GmbH & Co KG
2016-03-01
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doaj-43de4461f95a404b9cb5d0356298961d2020-11-25T00:12:42ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782016-03-013831015102910.1159/000443053443053Circulating MiR-19b-3p, MiR-134-5p and MiR-186-5p are Promising Novel Biomarkers for Early Diagnosis of Acute Myocardial InfarctionKe-Jing WangXin ZhaoYu-Zhou LiuQiu-Tang ZengXiao-Bo MaoSong-Nan LiMing ZhangChao JiangYou ZhouCheng QianKai-Ge FengHong-Quan GuanTing-Ting TangXiang ChengZhi-Jian ChenBackground/Aims: Recent studies have shown that circulating microRNAs (miRNAs) are emerging as promising biomarkers for cardiovascular diseases. This study aimed to determine whether miR-19b-3p, miR-134-5p and miR-186-5p can be used as novel indicators for acute myocardial infarction (AMI). Methods: To investigate the kinetic expression of the three selected miRNAs, we enrolled 18 patients with AMI and 20 matched controls. Plasma samples were collected from each participant, and total RNA was extracted. Quantitative real-time PCR and ELISA assays were used to investigate the expression of circulating miRNAs and cardiac troponin I (cTnI), respectively. Plasma samples from another age- and gender-matched cohort were collected to investigate the impact of medications for AMI on the expression of the selected miRNAs. Results: Levels of plasma miR-19b-3p, miR-134-5p and miR-186-5p were significantly increased in early stage of AMI. Plasma miR-19b-3p and miR-134-5p levels reached peak expression immediately after admission (T0), whereas miR-186-5p achieved peak expression at 4 h after T0. All of these times were earlier than the peak for cTnI (8 h after T0). In addition, all three miRNAs were positively correlated with cTnI. Receiver Operating Characteristic (ROC) analysis indicated that each single miRNA showed considerable diagnostic efficiency for predicting AMI. Furthermore, combining all three miRNAs in a panel increased the efficiency of distinguishing between patients with AMI and controls. Moreover, we found that heparin and medications for AMI did not impact the expression of these circulating miRNAs. Conclusion: Circulating miR-19b-3p, miR-134-5p and miR-186-5p could be considered promising novel diagnostic biomarkers for the early phase of AMI.http://www.karger.com/Article/FullText/443053Circulating microRNAsAcute myocardial infarctionDiagnostic biomarkers |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ke-Jing Wang Xin Zhao Yu-Zhou Liu Qiu-Tang Zeng Xiao-Bo Mao Song-Nan Li Ming Zhang Chao Jiang You Zhou Cheng Qian Kai-Ge Feng Hong-Quan Guan Ting-Ting Tang Xiang Cheng Zhi-Jian Chen |
spellingShingle |
Ke-Jing Wang Xin Zhao Yu-Zhou Liu Qiu-Tang Zeng Xiao-Bo Mao Song-Nan Li Ming Zhang Chao Jiang You Zhou Cheng Qian Kai-Ge Feng Hong-Quan Guan Ting-Ting Tang Xiang Cheng Zhi-Jian Chen Circulating MiR-19b-3p, MiR-134-5p and MiR-186-5p are Promising Novel Biomarkers for Early Diagnosis of Acute Myocardial Infarction Cellular Physiology and Biochemistry Circulating microRNAs Acute myocardial infarction Diagnostic biomarkers |
author_facet |
Ke-Jing Wang Xin Zhao Yu-Zhou Liu Qiu-Tang Zeng Xiao-Bo Mao Song-Nan Li Ming Zhang Chao Jiang You Zhou Cheng Qian Kai-Ge Feng Hong-Quan Guan Ting-Ting Tang Xiang Cheng Zhi-Jian Chen |
author_sort |
Ke-Jing Wang |
title |
Circulating MiR-19b-3p, MiR-134-5p and MiR-186-5p are Promising Novel Biomarkers for Early Diagnosis of Acute Myocardial Infarction |
title_short |
Circulating MiR-19b-3p, MiR-134-5p and MiR-186-5p are Promising Novel Biomarkers for Early Diagnosis of Acute Myocardial Infarction |
title_full |
Circulating MiR-19b-3p, MiR-134-5p and MiR-186-5p are Promising Novel Biomarkers for Early Diagnosis of Acute Myocardial Infarction |
title_fullStr |
Circulating MiR-19b-3p, MiR-134-5p and MiR-186-5p are Promising Novel Biomarkers for Early Diagnosis of Acute Myocardial Infarction |
title_full_unstemmed |
Circulating MiR-19b-3p, MiR-134-5p and MiR-186-5p are Promising Novel Biomarkers for Early Diagnosis of Acute Myocardial Infarction |
title_sort |
circulating mir-19b-3p, mir-134-5p and mir-186-5p are promising novel biomarkers for early diagnosis of acute myocardial infarction |
publisher |
Cell Physiol Biochem Press GmbH & Co KG |
series |
Cellular Physiology and Biochemistry |
issn |
1015-8987 1421-9778 |
publishDate |
2016-03-01 |
description |
Background/Aims: Recent studies have shown that circulating microRNAs (miRNAs) are emerging as promising biomarkers for cardiovascular diseases. This study aimed to determine whether miR-19b-3p, miR-134-5p and miR-186-5p can be used as novel indicators for acute myocardial infarction (AMI). Methods: To investigate the kinetic expression of the three selected miRNAs, we enrolled 18 patients with AMI and 20 matched controls. Plasma samples were collected from each participant, and total RNA was extracted. Quantitative real-time PCR and ELISA assays were used to investigate the expression of circulating miRNAs and cardiac troponin I (cTnI), respectively. Plasma samples from another age- and gender-matched cohort were collected to investigate the impact of medications for AMI on the expression of the selected miRNAs. Results: Levels of plasma miR-19b-3p, miR-134-5p and miR-186-5p were significantly increased in early stage of AMI. Plasma miR-19b-3p and miR-134-5p levels reached peak expression immediately after admission (T0), whereas miR-186-5p achieved peak expression at 4 h after T0. All of these times were earlier than the peak for cTnI (8 h after T0). In addition, all three miRNAs were positively correlated with cTnI. Receiver Operating Characteristic (ROC) analysis indicated that each single miRNA showed considerable diagnostic efficiency for predicting AMI. Furthermore, combining all three miRNAs in a panel increased the efficiency of distinguishing between patients with AMI and controls. Moreover, we found that heparin and medications for AMI did not impact the expression of these circulating miRNAs. Conclusion: Circulating miR-19b-3p, miR-134-5p and miR-186-5p could be considered promising novel diagnostic biomarkers for the early phase of AMI. |
topic |
Circulating microRNAs Acute myocardial infarction Diagnostic biomarkers |
url |
http://www.karger.com/Article/FullText/443053 |
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