Reproducible Molecularly Imprinted Piezoelectric Sensor for Accurate and Sensitive Detection of Ractopamine in Swine and Feed Products
This paper describes the development of a reproducible molecularly imprinted piezoelectric sensor for the accurate and sensitive detection of ractopamine (RAC) in swine and feed products. The synthesized molecularly imprinted polymer (MIP) was directly immobilized on the surface of a quartz crystal...
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doaj-4408f8da935146b0854251c0bd3179dd2020-11-25T00:50:02ZengMDPI AGSensors1424-82202018-06-01186187010.3390/s18061870s18061870Reproducible Molecularly Imprinted Piezoelectric Sensor for Accurate and Sensitive Detection of Ractopamine in Swine and Feed ProductsMingfei Pan0Rui Li1Leling Xu2Jingying Yang3Xiaoyuan Cui4Shuo Wang5Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, ChinaKey Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, ChinaKey Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, ChinaKey Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, ChinaKey Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, ChinaKey Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, ChinaThis paper describes the development of a reproducible molecularly imprinted piezoelectric sensor for the accurate and sensitive detection of ractopamine (RAC) in swine and feed products. The synthesized molecularly imprinted polymer (MIP) was directly immobilized on the surface of a quartz crystal microbalance (QCM) Au chip as the recognition element. The experimental parameters in the fabrication, measurement and regeneration process were evaluated in detail to produce an MIP-based piezoelectric sensor with high sensing capability. The developed piezoelectric sensor was verified to perform favorably in the RAC analysis of swine and feed products, with acceptable accuracy (recovery: 75.9–93.3%), precision [relative standard deviation (n = 3): 2.3–6.4%], and sensitivity [limit of detection: 0.46 ng g−1 (swine) and 0.38 ng g−1 (feed)]. This portable MIP-based chip for the piezoelectric sensing of RAC could be reused for at least 30 cycles and easily stored for a long time. These results demonstrated that the developed MIP-based piezoelectric sensor presents an accurate, sensitive and cost-effective method for the quantitative detection of RAC in complex samples. This research offers a promising strategy for the development of novel effective devices used for use in food safety analysis.http://www.mdpi.com/1424-8220/18/6/1870ractopaminemolecularly imprinted piezoelectric sensorswine and feed samplesquantitative detection |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mingfei Pan Rui Li Leling Xu Jingying Yang Xiaoyuan Cui Shuo Wang |
spellingShingle |
Mingfei Pan Rui Li Leling Xu Jingying Yang Xiaoyuan Cui Shuo Wang Reproducible Molecularly Imprinted Piezoelectric Sensor for Accurate and Sensitive Detection of Ractopamine in Swine and Feed Products Sensors ractopamine molecularly imprinted piezoelectric sensor swine and feed samples quantitative detection |
author_facet |
Mingfei Pan Rui Li Leling Xu Jingying Yang Xiaoyuan Cui Shuo Wang |
author_sort |
Mingfei Pan |
title |
Reproducible Molecularly Imprinted Piezoelectric Sensor for Accurate and Sensitive Detection of Ractopamine in Swine and Feed Products |
title_short |
Reproducible Molecularly Imprinted Piezoelectric Sensor for Accurate and Sensitive Detection of Ractopamine in Swine and Feed Products |
title_full |
Reproducible Molecularly Imprinted Piezoelectric Sensor for Accurate and Sensitive Detection of Ractopamine in Swine and Feed Products |
title_fullStr |
Reproducible Molecularly Imprinted Piezoelectric Sensor for Accurate and Sensitive Detection of Ractopamine in Swine and Feed Products |
title_full_unstemmed |
Reproducible Molecularly Imprinted Piezoelectric Sensor for Accurate and Sensitive Detection of Ractopamine in Swine and Feed Products |
title_sort |
reproducible molecularly imprinted piezoelectric sensor for accurate and sensitive detection of ractopamine in swine and feed products |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2018-06-01 |
description |
This paper describes the development of a reproducible molecularly imprinted piezoelectric sensor for the accurate and sensitive detection of ractopamine (RAC) in swine and feed products. The synthesized molecularly imprinted polymer (MIP) was directly immobilized on the surface of a quartz crystal microbalance (QCM) Au chip as the recognition element. The experimental parameters in the fabrication, measurement and regeneration process were evaluated in detail to produce an MIP-based piezoelectric sensor with high sensing capability. The developed piezoelectric sensor was verified to perform favorably in the RAC analysis of swine and feed products, with acceptable accuracy (recovery: 75.9–93.3%), precision [relative standard deviation (n = 3): 2.3–6.4%], and sensitivity [limit of detection: 0.46 ng g−1 (swine) and 0.38 ng g−1 (feed)]. This portable MIP-based chip for the piezoelectric sensing of RAC could be reused for at least 30 cycles and easily stored for a long time. These results demonstrated that the developed MIP-based piezoelectric sensor presents an accurate, sensitive and cost-effective method for the quantitative detection of RAC in complex samples. This research offers a promising strategy for the development of novel effective devices used for use in food safety analysis. |
topic |
ractopamine molecularly imprinted piezoelectric sensor swine and feed samples quantitative detection |
url |
http://www.mdpi.com/1424-8220/18/6/1870 |
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