Investigation of Chemical Composition, Antioxidant, Anticholinesterase and Anti-urease activities of Euphorbia helioscopia
In this study, chemical composition, antioxidant, anticholinesterase and anti-urease activities of the essential oil, hexane, acetone, methanol and water extracts of Euphorbia helioscopia were investigated. The chemical composition of the essential oil was analyzed by GC and GC/MS and β-cubebene (19...
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International Journal of Secondary Metabolite
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doaj-44100525d47f40d18097b8477db9616d2020-11-25T02:26:24ZengInternational Journal of Secondary MetaboliteInternational Journal of Secondary Metabolite2148-69052018-09-015325926910.21448/ijsm.433681618Investigation of Chemical Composition, Antioxidant, Anticholinesterase and Anti-urease activities of Euphorbia helioscopiaEbru DeveciGülsen Tel-ÇayanMehmet Emin DuruIn this study, chemical composition, antioxidant, anticholinesterase and anti-urease activities of the essential oil, hexane, acetone, methanol and water extracts of Euphorbia helioscopia were investigated. The chemical composition of the essential oil was analyzed by GC and GC/MS and β-cubebene (19.3 %), palmitic acid (12.2 %) and caryophyllene oxide (11.7 %) were identified as major compounds. The antioxidant activity of essential oil and extracts was performed by several methods such as β-carotene-linoleic acid, DPPH•, ABTS•+ radical scavenging, CUPRAC and metal chelating assays. The water extract showed higher antioxidant activity than BHA and α-tocopherol in β-carotene-linoleic acid, DPPH•, ABTS•+ and CUPRAC assays with IC50: 1.08±0.39, 15.57±0.48, 1.89±0.33 and 12.50±0.11 μg/mL values, respectively. Also, the anticholinesterase and anti-urease activities were tested against acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and urease enzymes, spectrophotometrically. The acetone extract (81.23±0.58 %) showed very close BChE inhibitory activity to galantamine. The hexane extract (96.97±0.36 %) of E. helioscopia showed higher anti-urease activity than thiourea (96.93±0.17 %) whereas the essential oil (91.37±0.26 %) indicated very close anti-urease activity to the standard.https://dergipark.org.tr/tr/pub/ijsm/issue/37294/433681?publisher=ijateeuphorbia helioscopiaessential oilantioxidant activityanticholinesterase activityanti-urease activity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ebru Deveci Gülsen Tel-Çayan Mehmet Emin Duru |
spellingShingle |
Ebru Deveci Gülsen Tel-Çayan Mehmet Emin Duru Investigation of Chemical Composition, Antioxidant, Anticholinesterase and Anti-urease activities of Euphorbia helioscopia International Journal of Secondary Metabolite euphorbia helioscopia essential oil antioxidant activity anticholinesterase activity anti-urease activity |
author_facet |
Ebru Deveci Gülsen Tel-Çayan Mehmet Emin Duru |
author_sort |
Ebru Deveci |
title |
Investigation of Chemical Composition, Antioxidant, Anticholinesterase and Anti-urease activities of Euphorbia helioscopia |
title_short |
Investigation of Chemical Composition, Antioxidant, Anticholinesterase and Anti-urease activities of Euphorbia helioscopia |
title_full |
Investigation of Chemical Composition, Antioxidant, Anticholinesterase and Anti-urease activities of Euphorbia helioscopia |
title_fullStr |
Investigation of Chemical Composition, Antioxidant, Anticholinesterase and Anti-urease activities of Euphorbia helioscopia |
title_full_unstemmed |
Investigation of Chemical Composition, Antioxidant, Anticholinesterase and Anti-urease activities of Euphorbia helioscopia |
title_sort |
investigation of chemical composition, antioxidant, anticholinesterase and anti-urease activities of euphorbia helioscopia |
publisher |
International Journal of Secondary Metabolite |
series |
International Journal of Secondary Metabolite |
issn |
2148-6905 |
publishDate |
2018-09-01 |
description |
In this
study, chemical composition, antioxidant, anticholinesterase and anti-urease activities
of the essential oil, hexane, acetone, methanol and water extracts of Euphorbia helioscopia were investigated.
The chemical composition of the essential oil was analyzed by GC and GC/MS and β-cubebene (19.3 %), palmitic acid (12.2
%) and caryophyllene oxide (11.7 %) were identified as major compounds. The antioxidant
activity of essential oil and extracts was performed by several methods such as
β-carotene-linoleic acid, DPPH•, ABTS•+ radical scavenging,
CUPRAC and metal chelating assays. The water extract showed higher antioxidant activity
than BHA and α-tocopherol in β-carotene-linoleic acid, DPPH•, ABTS•+
and CUPRAC assays with IC50: 1.08±0.39, 15.57±0.48, 1.89±0.33 and 12.50±0.11
μg/mL values, respectively. Also, the anticholinesterase and anti-urease activities
were tested against acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and
urease enzymes, spectrophotometrically. The acetone extract (81.23±0.58 %) showed
very close BChE inhibitory activity to galantamine. The hexane extract (96.97±0.36
%) of E. helioscopia showed higher anti-urease
activity than thiourea (96.93±0.17 %) whereas the essential oil (91.37±0.26 %) indicated
very close anti-urease activity to the standard. |
topic |
euphorbia helioscopia essential oil antioxidant activity anticholinesterase activity anti-urease activity |
url |
https://dergipark.org.tr/tr/pub/ijsm/issue/37294/433681?publisher=ijate |
work_keys_str_mv |
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1724847398670303232 |