Comparison of Sendai Virus-Mediated Gene Transfer Efficiency to Adhesive and Floating Adipose Tissue-Derived Stem Cells
Sendai virus (SeV) vectors have potential clinical applications because they can efficiently introduce foreign genes without toxicity into various organs. A recent study reported the green fluorescent protein (GFP) gene transfer to adipose tissue-derived stem cells (ASCs) with SeV vectors results in...
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doaj-4411192090a44c9daf8d87c4ea0ba6972020-11-25T03:44:08ZengSAGE PublishingCell Transplantation0963-68971555-38922009-05-011810.1177/096368970901805-616Comparison of Sendai Virus-Mediated Gene Transfer Efficiency to Adhesive and Floating Adipose Tissue-Derived Stem CellsHiroshi Yukawa0Hirofumi Noguchi1Koichi Oishi2Makoto Inoue3Mamoru Hasegawa4Michinari Hamaguchi5Nobuyuki Hamajima6Shuji Hayashi7Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya 461-0047, JapanBaylor All Saints Medical Center, Baylor Research Institute, Dallas, TX 75204, USADepartment of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya 461-0047, JapanDNAVEC Corporation, Tsukuba-city, Ibaraki, 305-0856, JapanDNAVEC Corporation, Tsukuba-city, Ibaraki, 305-0856, JapanDivision of Cancer Biology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, JapanDepartment of Preventive Medicine, Biostatistics and Medical Decision Making, Nagoya University Graduate School of Medicine, Nagoya 466-8550, JapanDepartment of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya 461-0047, JapanSendai virus (SeV) vectors have potential clinical applications because they can efficiently introduce foreign genes without toxicity into various organs. A recent study reported the green fluorescent protein (GFP) gene transfer to adipose tissue-derived stem cells (ASCs) with SeV vectors results in more efficient expression of GFP than AdV and identified the preservation of the multilineage potential of ASCs transfected with SeV vectors. This study assessed the gene transfer efficiency to floating ASCs with SeV vectors. Although a slight cytotoxicity was observed, the efficiency of gene transfer to cells in the floating state was much higher at all times and all concentrations at MOIs of 2, 10, and 20 than in the adhesion state. Moreover, ASCs transfected with SeV vectors in floating state have the same potential for their differentiation into specific tissues, such as adipocytes and osteocytes, as untransfected ASCs. These data suggest that SeV transfection to ASCs in the floating state could therefore be useful for gene transfer technology.https://doi.org/10.1177/096368970901805-616 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hiroshi Yukawa Hirofumi Noguchi Koichi Oishi Makoto Inoue Mamoru Hasegawa Michinari Hamaguchi Nobuyuki Hamajima Shuji Hayashi |
spellingShingle |
Hiroshi Yukawa Hirofumi Noguchi Koichi Oishi Makoto Inoue Mamoru Hasegawa Michinari Hamaguchi Nobuyuki Hamajima Shuji Hayashi Comparison of Sendai Virus-Mediated Gene Transfer Efficiency to Adhesive and Floating Adipose Tissue-Derived Stem Cells Cell Transplantation |
author_facet |
Hiroshi Yukawa Hirofumi Noguchi Koichi Oishi Makoto Inoue Mamoru Hasegawa Michinari Hamaguchi Nobuyuki Hamajima Shuji Hayashi |
author_sort |
Hiroshi Yukawa |
title |
Comparison of Sendai Virus-Mediated Gene Transfer Efficiency to Adhesive and Floating Adipose Tissue-Derived Stem Cells |
title_short |
Comparison of Sendai Virus-Mediated Gene Transfer Efficiency to Adhesive and Floating Adipose Tissue-Derived Stem Cells |
title_full |
Comparison of Sendai Virus-Mediated Gene Transfer Efficiency to Adhesive and Floating Adipose Tissue-Derived Stem Cells |
title_fullStr |
Comparison of Sendai Virus-Mediated Gene Transfer Efficiency to Adhesive and Floating Adipose Tissue-Derived Stem Cells |
title_full_unstemmed |
Comparison of Sendai Virus-Mediated Gene Transfer Efficiency to Adhesive and Floating Adipose Tissue-Derived Stem Cells |
title_sort |
comparison of sendai virus-mediated gene transfer efficiency to adhesive and floating adipose tissue-derived stem cells |
publisher |
SAGE Publishing |
series |
Cell Transplantation |
issn |
0963-6897 1555-3892 |
publishDate |
2009-05-01 |
description |
Sendai virus (SeV) vectors have potential clinical applications because they can efficiently introduce foreign genes without toxicity into various organs. A recent study reported the green fluorescent protein (GFP) gene transfer to adipose tissue-derived stem cells (ASCs) with SeV vectors results in more efficient expression of GFP than AdV and identified the preservation of the multilineage potential of ASCs transfected with SeV vectors. This study assessed the gene transfer efficiency to floating ASCs with SeV vectors. Although a slight cytotoxicity was observed, the efficiency of gene transfer to cells in the floating state was much higher at all times and all concentrations at MOIs of 2, 10, and 20 than in the adhesion state. Moreover, ASCs transfected with SeV vectors in floating state have the same potential for their differentiation into specific tissues, such as adipocytes and osteocytes, as untransfected ASCs. These data suggest that SeV transfection to ASCs in the floating state could therefore be useful for gene transfer technology. |
url |
https://doi.org/10.1177/096368970901805-616 |
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