Use of AFLP markers for molecular characterization of two crops with agricultural interests

Use of AFLP markers for molecular characterization of two crops with agricultural interests

The AFLP technique is based on the selective amplification of restriction fragment by polymerase chain reaction (PCR) and consists of four basic steps; digestion with restriction enzymes of deoxyribonucleic acid (DNA), adapter ligation with fragments of DNA and amplification of the fragments obtaine...

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Main Authors: Luis Rojas, Jorge López, Rafael Gómez-Kosky, Orelvis Portal
Format: Article
Language:English
Published: Universidad Central Marta Abreu de Las Villas 2007-04-01
Series:Biotecnología Vegetal
Online Access:https://revista.ibp.co.cu/index.php/BV/article/view/363
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spelling doaj-4429b4c31abf4f34917fe2798f61b7192020-11-24T21:51:51ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472007-04-0172336Use of AFLP markers for molecular characterization of two crops with agricultural interestsLuis RojasJorge LópezRafael Gómez-KoskyOrelvis PortalThe AFLP technique is based on the selective amplification of restriction fragment by polymerase chain reaction (PCR) and consists of four basic steps; digestion with restriction enzymes of deoxyribonucleic acid (DNA), adapter ligation with fragments of DNA and amplification of the fragments obtained in the earlier steps using combinations of selective primers and detection of polymorphism using radioisotopes, fluorescent dyes or silver staining. It is widely used in the evaluation of genetic diversity, analysis of genetic distance, DNA fingerprint, germoplasm collections analysis, genetic maps construction and markers diagnostic monitoring. In order to characterize molecularly two species of crops with agricultural interests (sugar cane and banana) were generated AFLP markers. In this study was demonstrated the ability to identify variations in the genome of crops studied. Key words: Musa, ‘Navolean’, Saccharum officinarum, sugarcanehttps://revista.ibp.co.cu/index.php/BV/article/view/363
collection DOAJ
language English
format Article
sources DOAJ
author Luis Rojas
Jorge López
Rafael Gómez-Kosky
Orelvis Portal
spellingShingle Luis Rojas
Jorge López
Rafael Gómez-Kosky
Orelvis Portal
Use of AFLP markers for molecular characterization of two crops with agricultural interests
Biotecnología Vegetal
author_facet Luis Rojas
Jorge López
Rafael Gómez-Kosky
Orelvis Portal
author_sort Luis Rojas
title Use of AFLP markers for molecular characterization of two crops with agricultural interests
title_short Use of AFLP markers for molecular characterization of two crops with agricultural interests
title_full Use of AFLP markers for molecular characterization of two crops with agricultural interests
title_fullStr Use of AFLP markers for molecular characterization of two crops with agricultural interests
title_full_unstemmed Use of AFLP markers for molecular characterization of two crops with agricultural interests
title_sort use of aflp markers for molecular characterization of two crops with agricultural interests
publisher Universidad Central Marta Abreu de Las Villas
series Biotecnología Vegetal
issn 1609-1841
2074-8647
publishDate 2007-04-01
description The AFLP technique is based on the selective amplification of restriction fragment by polymerase chain reaction (PCR) and consists of four basic steps; digestion with restriction enzymes of deoxyribonucleic acid (DNA), adapter ligation with fragments of DNA and amplification of the fragments obtained in the earlier steps using combinations of selective primers and detection of polymorphism using radioisotopes, fluorescent dyes or silver staining. It is widely used in the evaluation of genetic diversity, analysis of genetic distance, DNA fingerprint, germoplasm collections analysis, genetic maps construction and markers diagnostic monitoring. In order to characterize molecularly two species of crops with agricultural interests (sugar cane and banana) were generated AFLP markers. In this study was demonstrated the ability to identify variations in the genome of crops studied. Key words: Musa, ‘Navolean’, Saccharum officinarum, sugarcane
url https://revista.ibp.co.cu/index.php/BV/article/view/363
work_keys_str_mv AT luisrojas useofaflpmarkersformolecularcharacterizationoftwocropswithagriculturalinterests
AT jorgelopez useofaflpmarkersformolecularcharacterizationoftwocropswithagriculturalinterests
AT rafaelgomezkosky useofaflpmarkersformolecularcharacterizationoftwocropswithagriculturalinterests
AT orelvisportal useofaflpmarkersformolecularcharacterizationoftwocropswithagriculturalinterests
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