Human Laryngeal Mucus from the Vocal Folds: Rheological Characterization by Particle Tracking Microrheology and Oscillatory Shear Rheology
Mucus consistency affects voice physiology and is connected to voice disorders. Nevertheless, the rheological characteristics of human laryngeal mucus from the vocal folds remain unknown. Knowledge about mucus viscoelasticity enables fabrication of artificial mucus with natural properties, more real...
Main Authors: | , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-03-01
|
Series: | Applied Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/2076-3417/11/7/3011 |
id |
doaj-442b1819402345128287a444a6366367 |
---|---|
record_format |
Article |
spelling |
doaj-442b1819402345128287a444a63663672021-03-28T00:03:01ZengMDPI AGApplied Sciences2076-34172021-03-01113011301110.3390/app11073011Human Laryngeal Mucus from the Vocal Folds: Rheological Characterization by Particle Tracking Microrheology and Oscillatory Shear RheologyGregor Peters0Olaf Wendler1David Böhringer2Antoniu-Oreste Gostian3Sarina K. Müller4Herbert Canziani5Nicolas Hesse6Marion Semmler7David A. Berry8Stefan Kniesburges9Wolfgang Peukert10Michael Döllinger11Department of Otorhinolaryngology, Div. of Phoniatrics and Pediatric Audiology, University Hospital Erlangen, Friedrich-Alexander-University Erlangen-Nürnberg, 91054 Erlangen, GermanyDepartment of Otorhinolaryngology, Div. of Phoniatrics and Pediatric Audiology, University Hospital Erlangen, Friedrich-Alexander-University Erlangen-Nürnberg, 91054 Erlangen, GermanyBiophysics Group, Department of Physics, Friedrich-Alexander-University Erlangen-Nürnberg, 91052 Erlangen, GermanyDepartment of Otorhinolaryngology, University Hospital Erlangen, Friedrich-Alexander-University Erlangen-Nürnberg, 91054 Erlangen, GermanyDepartment of Otorhinolaryngology, University Hospital Erlangen, Friedrich-Alexander-University Erlangen-Nürnberg, 91054 Erlangen, GermanyDepartment of Chemical and Biological Engineering, Chair of Particle Technology, Friedrich-Alexander-University Erlangen-Nürnberg, 91058 Erlangen, GermanyDepartment of Chemical and Biological Engineering, Chair of Particle Technology, Friedrich-Alexander-University Erlangen-Nürnberg, 91058 Erlangen, GermanyDepartment of Otorhinolaryngology, Div. of Phoniatrics and Pediatric Audiology, University Hospital Erlangen, Friedrich-Alexander-University Erlangen-Nürnberg, 91054 Erlangen, GermanyDepartment of Head and Neck Surgery, David Geffen School of Medicine at University of California Los Angeles, Los Angeles, CA 90024, USADepartment of Otorhinolaryngology, Div. of Phoniatrics and Pediatric Audiology, University Hospital Erlangen, Friedrich-Alexander-University Erlangen-Nürnberg, 91054 Erlangen, GermanyDepartment of Chemical and Biological Engineering, Chair of Particle Technology, Friedrich-Alexander-University Erlangen-Nürnberg, 91058 Erlangen, GermanyDepartment of Otorhinolaryngology, Div. of Phoniatrics and Pediatric Audiology, University Hospital Erlangen, Friedrich-Alexander-University Erlangen-Nürnberg, 91054 Erlangen, GermanyMucus consistency affects voice physiology and is connected to voice disorders. Nevertheless, the rheological characteristics of human laryngeal mucus from the vocal folds remain unknown. Knowledge about mucus viscoelasticity enables fabrication of artificial mucus with natural properties, more realistic ex-vivo experiments and promotes a better understanding and improved treatment of dysphonia with regard to mucus consistency. We studied human laryngeal mucus samples from the vocal folds with two complementary approaches: 19 samples were successfully applied to particle tracking microrheology (PTM) and five additional samples to oscillatory shear rheology (OSR). Mucus was collected by experienced laryngologists from patients together with demographic data. The analysis of the viscoelasticity revealed diversity among the investigated mucus samples according to their rigidity (absolute G’ and G”). Moreover some samples revealed throughout solid-like character (G’ > G”), whereas some underwent a change from solid-like to liquid-like (G’ < G”). This led to a subdivision into three groups. We assume that the reason for the differences is a variation in the hydration level of the mucus, which affects the mucin concentration and network formation factors of the mucin mesh. The demographic data could not be correlated to the differences, except for the smoking behavior. Mucus of predominant liquid-like character was associated with current smokers.https://www.mdpi.com/2076-3417/11/7/3011human laryngeal mucusviscoelasticityparticle tracking microrheologyoscillatory shear rheologyvocal folds |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gregor Peters Olaf Wendler David Böhringer Antoniu-Oreste Gostian Sarina K. Müller Herbert Canziani Nicolas Hesse Marion Semmler David A. Berry Stefan Kniesburges Wolfgang Peukert Michael Döllinger |
spellingShingle |
Gregor Peters Olaf Wendler David Böhringer Antoniu-Oreste Gostian Sarina K. Müller Herbert Canziani Nicolas Hesse Marion Semmler David A. Berry Stefan Kniesburges Wolfgang Peukert Michael Döllinger Human Laryngeal Mucus from the Vocal Folds: Rheological Characterization by Particle Tracking Microrheology and Oscillatory Shear Rheology Applied Sciences human laryngeal mucus viscoelasticity particle tracking microrheology oscillatory shear rheology vocal folds |
author_facet |
Gregor Peters Olaf Wendler David Böhringer Antoniu-Oreste Gostian Sarina K. Müller Herbert Canziani Nicolas Hesse Marion Semmler David A. Berry Stefan Kniesburges Wolfgang Peukert Michael Döllinger |
author_sort |
Gregor Peters |
title |
Human Laryngeal Mucus from the Vocal Folds: Rheological Characterization by Particle Tracking Microrheology and Oscillatory Shear Rheology |
title_short |
Human Laryngeal Mucus from the Vocal Folds: Rheological Characterization by Particle Tracking Microrheology and Oscillatory Shear Rheology |
title_full |
Human Laryngeal Mucus from the Vocal Folds: Rheological Characterization by Particle Tracking Microrheology and Oscillatory Shear Rheology |
title_fullStr |
Human Laryngeal Mucus from the Vocal Folds: Rheological Characterization by Particle Tracking Microrheology and Oscillatory Shear Rheology |
title_full_unstemmed |
Human Laryngeal Mucus from the Vocal Folds: Rheological Characterization by Particle Tracking Microrheology and Oscillatory Shear Rheology |
title_sort |
human laryngeal mucus from the vocal folds: rheological characterization by particle tracking microrheology and oscillatory shear rheology |
publisher |
MDPI AG |
series |
Applied Sciences |
issn |
2076-3417 |
publishDate |
2021-03-01 |
description |
Mucus consistency affects voice physiology and is connected to voice disorders. Nevertheless, the rheological characteristics of human laryngeal mucus from the vocal folds remain unknown. Knowledge about mucus viscoelasticity enables fabrication of artificial mucus with natural properties, more realistic ex-vivo experiments and promotes a better understanding and improved treatment of dysphonia with regard to mucus consistency. We studied human laryngeal mucus samples from the vocal folds with two complementary approaches: 19 samples were successfully applied to particle tracking microrheology (PTM) and five additional samples to oscillatory shear rheology (OSR). Mucus was collected by experienced laryngologists from patients together with demographic data. The analysis of the viscoelasticity revealed diversity among the investigated mucus samples according to their rigidity (absolute G’ and G”). Moreover some samples revealed throughout solid-like character (G’ > G”), whereas some underwent a change from solid-like to liquid-like (G’ < G”). This led to a subdivision into three groups. We assume that the reason for the differences is a variation in the hydration level of the mucus, which affects the mucin concentration and network formation factors of the mucin mesh. The demographic data could not be correlated to the differences, except for the smoking behavior. Mucus of predominant liquid-like character was associated with current smokers. |
topic |
human laryngeal mucus viscoelasticity particle tracking microrheology oscillatory shear rheology vocal folds |
url |
https://www.mdpi.com/2076-3417/11/7/3011 |
work_keys_str_mv |
AT gregorpeters humanlaryngealmucusfromthevocalfoldsrheologicalcharacterizationbyparticletrackingmicrorheologyandoscillatoryshearrheology AT olafwendler humanlaryngealmucusfromthevocalfoldsrheologicalcharacterizationbyparticletrackingmicrorheologyandoscillatoryshearrheology AT davidbohringer humanlaryngealmucusfromthevocalfoldsrheologicalcharacterizationbyparticletrackingmicrorheologyandoscillatoryshearrheology AT antoniuorestegostian humanlaryngealmucusfromthevocalfoldsrheologicalcharacterizationbyparticletrackingmicrorheologyandoscillatoryshearrheology AT sarinakmuller humanlaryngealmucusfromthevocalfoldsrheologicalcharacterizationbyparticletrackingmicrorheologyandoscillatoryshearrheology AT herbertcanziani humanlaryngealmucusfromthevocalfoldsrheologicalcharacterizationbyparticletrackingmicrorheologyandoscillatoryshearrheology AT nicolashesse humanlaryngealmucusfromthevocalfoldsrheologicalcharacterizationbyparticletrackingmicrorheologyandoscillatoryshearrheology AT marionsemmler humanlaryngealmucusfromthevocalfoldsrheologicalcharacterizationbyparticletrackingmicrorheologyandoscillatoryshearrheology AT davidaberry humanlaryngealmucusfromthevocalfoldsrheologicalcharacterizationbyparticletrackingmicrorheologyandoscillatoryshearrheology AT stefankniesburges humanlaryngealmucusfromthevocalfoldsrheologicalcharacterizationbyparticletrackingmicrorheologyandoscillatoryshearrheology AT wolfgangpeukert humanlaryngealmucusfromthevocalfoldsrheologicalcharacterizationbyparticletrackingmicrorheologyandoscillatoryshearrheology AT michaeldollinger humanlaryngealmucusfromthevocalfoldsrheologicalcharacterizationbyparticletrackingmicrorheologyandoscillatoryshearrheology |
_version_ |
1724200583986937856 |