Targeted Nanoparticles Harboring Jasmine-Oil-Entrapped Paclitaxel for Elimination of Lung Cancer Cells
Selectively targeted drug delivery systems are preferable chemotherapeutic platforms, as they specifically deliver the drug cargo into tumor cells, while minimizing untoward toxic effects. However, these delivery systems suffer from insufficient encapsulation efficiency (EE), encapsulation capacity...
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doaj-44333a57eeb14a00bceaf32ebacefad32021-01-21T00:04:48ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-01221019101910.3390/ijms22031019Targeted Nanoparticles Harboring Jasmine-Oil-Entrapped Paclitaxel for Elimination of Lung Cancer CellsShira Engelberg0Yuexi Lin1Yehuda G. Assaraf2Yoav D. Livney3The Laboratory of Biopolymers for Food and Health, Department of Biotechnology and Food Engineering, Technion—Israel Institute of Technology, Haifa 3200003, IsraelThe Laboratory of Biopolymers for Food and Health, Department of Biotechnology and Food Engineering, Technion—Israel Institute of Technology, Haifa 3200003, IsraelThe Fred Wyszkowski Cancer Research Laboratory, Department of Biology, Technion—Israel Institute of Technology, Haifa 3200003, IsraelThe Fred Wyszkowski Cancer Research Laboratory, Department of Biology, Technion—Israel Institute of Technology, Haifa 3200003, IsraelSelectively targeted drug delivery systems are preferable chemotherapeutic platforms, as they specifically deliver the drug cargo into tumor cells, while minimizing untoward toxic effects. However, these delivery systems suffer from insufficient encapsulation efficiency (EE), encapsulation capacity (EC), and premature drug release. Herein, we coencapsulated paclitaxel (PTX) and Jasmine oil (JO) within PEG-PCL nanoparticles (NPs), with an average diameter < 50 nm, selectively targeted to non-small cell lung cancer (NSCLC) cells, via S15-aptamer (APT) decoration. JO was selected as an “adhesive” oily core to enhance PTX entrapment, as JO and PTX share similar hydrophobicity and terpenoid structure. JO markedly enhanced EE of PTX from 23% to 87.8% and EC from 35 ± 6 to 74 ± 8 µg PTX/mg PEG-PCL. JO also markedly increased the residual amount of PTX after 69 h, from 18.3% to 65%. Moreover, PTX cytotoxicity against human NSCLC A549 cells was significantly enhanced due to the co-encapsulation with JO; the IC<sub>50</sub> value for PTX encapsulated within JO-containing APT-NPs was 20-fold lower than that for APT-NPs lacking JO. Remarkably, JO-containing APT-NPs displayed a 6-fold more potent cell-killing, relatively to the free-drug. Collectively, these findings reveal a marked synergistic contribution of JO to the cytotoxic activity of APT-NP-based systems, for targeted PTX delivery against NSCLC, which may be readily applied to various hydrophobic chemotherapeutics.https://www.mdpi.com/1422-0067/22/3/1019lung cancertargeted deliverynanoparticlesjasmine oilpaclitaxelcoencapsulation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shira Engelberg Yuexi Lin Yehuda G. Assaraf Yoav D. Livney |
spellingShingle |
Shira Engelberg Yuexi Lin Yehuda G. Assaraf Yoav D. Livney Targeted Nanoparticles Harboring Jasmine-Oil-Entrapped Paclitaxel for Elimination of Lung Cancer Cells International Journal of Molecular Sciences lung cancer targeted delivery nanoparticles jasmine oil paclitaxel coencapsulation |
author_facet |
Shira Engelberg Yuexi Lin Yehuda G. Assaraf Yoav D. Livney |
author_sort |
Shira Engelberg |
title |
Targeted Nanoparticles Harboring Jasmine-Oil-Entrapped Paclitaxel for Elimination of Lung Cancer Cells |
title_short |
Targeted Nanoparticles Harboring Jasmine-Oil-Entrapped Paclitaxel for Elimination of Lung Cancer Cells |
title_full |
Targeted Nanoparticles Harboring Jasmine-Oil-Entrapped Paclitaxel for Elimination of Lung Cancer Cells |
title_fullStr |
Targeted Nanoparticles Harboring Jasmine-Oil-Entrapped Paclitaxel for Elimination of Lung Cancer Cells |
title_full_unstemmed |
Targeted Nanoparticles Harboring Jasmine-Oil-Entrapped Paclitaxel for Elimination of Lung Cancer Cells |
title_sort |
targeted nanoparticles harboring jasmine-oil-entrapped paclitaxel for elimination of lung cancer cells |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-01-01 |
description |
Selectively targeted drug delivery systems are preferable chemotherapeutic platforms, as they specifically deliver the drug cargo into tumor cells, while minimizing untoward toxic effects. However, these delivery systems suffer from insufficient encapsulation efficiency (EE), encapsulation capacity (EC), and premature drug release. Herein, we coencapsulated paclitaxel (PTX) and Jasmine oil (JO) within PEG-PCL nanoparticles (NPs), with an average diameter < 50 nm, selectively targeted to non-small cell lung cancer (NSCLC) cells, via S15-aptamer (APT) decoration. JO was selected as an “adhesive” oily core to enhance PTX entrapment, as JO and PTX share similar hydrophobicity and terpenoid structure. JO markedly enhanced EE of PTX from 23% to 87.8% and EC from 35 ± 6 to 74 ± 8 µg PTX/mg PEG-PCL. JO also markedly increased the residual amount of PTX after 69 h, from 18.3% to 65%. Moreover, PTX cytotoxicity against human NSCLC A549 cells was significantly enhanced due to the co-encapsulation with JO; the IC<sub>50</sub> value for PTX encapsulated within JO-containing APT-NPs was 20-fold lower than that for APT-NPs lacking JO. Remarkably, JO-containing APT-NPs displayed a 6-fold more potent cell-killing, relatively to the free-drug. Collectively, these findings reveal a marked synergistic contribution of JO to the cytotoxic activity of APT-NP-based systems, for targeted PTX delivery against NSCLC, which may be readily applied to various hydrophobic chemotherapeutics. |
topic |
lung cancer targeted delivery nanoparticles jasmine oil paclitaxel coencapsulation |
url |
https://www.mdpi.com/1422-0067/22/3/1019 |
work_keys_str_mv |
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