Radiosensitization by Hyperthermia: The Effects of Temperature, Sequence, and Time Interval in Cervical Cell Lines

Cervical cancers are almost exclusively caused by an infection with the human papillomavirus (HPV). When patients suffering from cervical cancer have contraindications for chemoradiotherapy, radiotherapy combined with hyperthermia is a good treatment option<b>. </b>Radiation-induced DNA...

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Main Authors:	Xionge Mei, Rosemarie ten Cate, Caspar M. van Leeuwen, Hans M. Rodermond, Lidewij de Leeuw, Dionysia Dimitrakopoulou, Lukas J.A. Stalpers, Johannes Crezee, H. Petra Kok, Nicolaas A.P. Franken, Arlene L. Oei
Format:	Article
Language:	English
Published:	MDPI AG 2020-03-01
Series:	Cancers
Subjects:	human papillomavirus ionizing radiation hyperthermia time interval sequence
Online Access:	https://www.mdpi.com/2072-6694/12/3/582

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spelling	doaj-444991cb217c4d67b5ab15dfaed6f9022020-11-25T02:57:37ZengMDPI AGCancers2072-66942020-03-0112358210.3390/cancers12030582cancers12030582Radiosensitization by Hyperthermia: The Effects of Temperature, Sequence, and Time Interval in Cervical Cell LinesXionge Mei0Rosemarie ten Cate1Caspar M. van Leeuwen2Hans M. Rodermond3Lidewij de Leeuw4Dionysia Dimitrakopoulou5Lukas J.A. Stalpers6Johannes Crezee7H. Petra Kok8Nicolaas A.P. Franken9Arlene L. Oei10Laboratory for Experimental Oncology and Radiobiology (LEXOR), Center for Experimental Molecular Medicine, Amsterdam University Medical Centers, P.O. Box 22700, 1100 DE Amsterdam, The NetherlandsLaboratory for Experimental Oncology and Radiobiology (LEXOR), Center for Experimental Molecular Medicine, Amsterdam University Medical Centers, P.O. Box 22700, 1100 DE Amsterdam, The NetherlandsDepartment of Radiotherapy, Amsterdam University Medical Centers, P.O. Box 22700, 1100 DE Amsterdam, The NetherlandsLaboratory for Experimental Oncology and Radiobiology (LEXOR), Center for Experimental Molecular Medicine, Amsterdam University Medical Centers, P.O. Box 22700, 1100 DE Amsterdam, The NetherlandsLaboratory for Experimental Oncology and Radiobiology (LEXOR), Center for Experimental Molecular Medicine, Amsterdam University Medical Centers, P.O. Box 22700, 1100 DE Amsterdam, The NetherlandsLaboratory for Experimental Oncology and Radiobiology (LEXOR), Center for Experimental Molecular Medicine, Amsterdam University Medical Centers, P.O. Box 22700, 1100 DE Amsterdam, The NetherlandsLaboratory for Experimental Oncology and Radiobiology (LEXOR), Center for Experimental Molecular Medicine, Amsterdam University Medical Centers, P.O. Box 22700, 1100 DE Amsterdam, The NetherlandsDepartment of Radiotherapy, Amsterdam University Medical Centers, P.O. Box 22700, 1100 DE Amsterdam, The NetherlandsDepartment of Radiotherapy, Amsterdam University Medical Centers, P.O. Box 22700, 1100 DE Amsterdam, The NetherlandsLaboratory for Experimental Oncology and Radiobiology (LEXOR), Center for Experimental Molecular Medicine, Amsterdam University Medical Centers, P.O. Box 22700, 1100 DE Amsterdam, The NetherlandsLaboratory for Experimental Oncology and Radiobiology (LEXOR), Center for Experimental Molecular Medicine, Amsterdam University Medical Centers, P.O. Box 22700, 1100 DE Amsterdam, The NetherlandsCervical cancers are almost exclusively caused by an infection with the human papillomavirus (HPV). When patients suffering from cervical cancer have contraindications for chemoradiotherapy, radiotherapy combined with hyperthermia is a good treatment option<b>. </b>Radiation-induced DNA breaks can be repaired by nonhomologous end-joining (NHEJ) or homologous recombination (HR). Hyperthermia can temporarily inactivate homologous recombination. Therefore, combining radiotherapy with hyperthermia can result in the persistence of more fatal radiation-induced DNA breaks. However, there is no consensus on the optimal sequence of radiotherapy and hyperthermia and the optimal time interval between these modalities. Moreover, the temperature of hyperthermia and HPV-type may also be important in radiosensitization by hyperthermia. In this study we thoroughly investigated the impact of different temperatures (37−42 °C), and the sequence of and time interval (0 up to 4 h) between ionizing radiation and hyperthermia on HPV16<sup>+</sup>: SiHa, Caski; HPV18<sup>+</sup>: HeLa, C4I; and HPV<sup>−</sup>: C33A, HT3 cervical cancer cell lines. Our results demonstrate that a short time interval between treatments caused more unrepaired DNA damages and more cell kill, especially at higher temperatures. Although hyperthermia before ionizing radiation may result in slightly more DNA damage, the sequence between hyperthermia and ionizing radiation yielded similar effects on cell survival.https://www.mdpi.com/2072-6694/12/3/582human papillomavirusionizing radiationhyperthermiatime intervalsequence
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Xionge Mei Rosemarie ten Cate Caspar M. van Leeuwen Hans M. Rodermond Lidewij de Leeuw Dionysia Dimitrakopoulou Lukas J.A. Stalpers Johannes Crezee H. Petra Kok Nicolaas A.P. Franken Arlene L. Oei
spellingShingle	Xionge Mei Rosemarie ten Cate Caspar M. van Leeuwen Hans M. Rodermond Lidewij de Leeuw Dionysia Dimitrakopoulou Lukas J.A. Stalpers Johannes Crezee H. Petra Kok Nicolaas A.P. Franken Arlene L. Oei Radiosensitization by Hyperthermia: The Effects of Temperature, Sequence, and Time Interval in Cervical Cell Lines Cancers human papillomavirus ionizing radiation hyperthermia time interval sequence
author_facet	Xionge Mei Rosemarie ten Cate Caspar M. van Leeuwen Hans M. Rodermond Lidewij de Leeuw Dionysia Dimitrakopoulou Lukas J.A. Stalpers Johannes Crezee H. Petra Kok Nicolaas A.P. Franken Arlene L. Oei
author_sort	Xionge Mei
title	Radiosensitization by Hyperthermia: The Effects of Temperature, Sequence, and Time Interval in Cervical Cell Lines
title_short	Radiosensitization by Hyperthermia: The Effects of Temperature, Sequence, and Time Interval in Cervical Cell Lines
title_full	Radiosensitization by Hyperthermia: The Effects of Temperature, Sequence, and Time Interval in Cervical Cell Lines
title_fullStr	Radiosensitization by Hyperthermia: The Effects of Temperature, Sequence, and Time Interval in Cervical Cell Lines
title_full_unstemmed	Radiosensitization by Hyperthermia: The Effects of Temperature, Sequence, and Time Interval in Cervical Cell Lines
title_sort	radiosensitization by hyperthermia: the effects of temperature, sequence, and time interval in cervical cell lines
publisher	MDPI AG
series	Cancers
issn	2072-6694
publishDate	2020-03-01
description	Cervical cancers are almost exclusively caused by an infection with the human papillomavirus (HPV). When patients suffering from cervical cancer have contraindications for chemoradiotherapy, radiotherapy combined with hyperthermia is a good treatment option<b>. </b>Radiation-induced DNA breaks can be repaired by nonhomologous end-joining (NHEJ) or homologous recombination (HR). Hyperthermia can temporarily inactivate homologous recombination. Therefore, combining radiotherapy with hyperthermia can result in the persistence of more fatal radiation-induced DNA breaks. However, there is no consensus on the optimal sequence of radiotherapy and hyperthermia and the optimal time interval between these modalities. Moreover, the temperature of hyperthermia and HPV-type may also be important in radiosensitization by hyperthermia. In this study we thoroughly investigated the impact of different temperatures (37−42 °C), and the sequence of and time interval (0 up to 4 h) between ionizing radiation and hyperthermia on HPV16<sup>+</sup>: SiHa, Caski; HPV18<sup>+</sup>: HeLa, C4I; and HPV<sup>−</sup>: C33A, HT3 cervical cancer cell lines. Our results demonstrate that a short time interval between treatments caused more unrepaired DNA damages and more cell kill, especially at higher temperatures. Although hyperthermia before ionizing radiation may result in slightly more DNA damage, the sequence between hyperthermia and ionizing radiation yielded similar effects on cell survival.
topic	human papillomavirus ionizing radiation hyperthermia time interval sequence
url	https://www.mdpi.com/2072-6694/12/3/582
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Radiosensitization by Hyperthermia: The Effects of Temperature, Sequence, and Time Interval in Cervical Cell Lines

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