Separation of Biojenic Amines Using Dansyl Cloride Derivatization and Mixed Micellar Liquid Chromatography

• Main Authors: Mohammad Reza Hadjmohammadi, Soghra Jafari
• Format: Article
• Language: English
• Published: Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR 2006-03-01
• Series: Iranian Journal of Chemistry & Chemical Engineering
• Subjects: biogenic amines; hplc; micellar liquid chromatography; mixed micelles
• Online Access: http://www.ijcce.ac.ir/article_8094_8db9366f82bdde0254e41dee7e0e373b.pdf

Separation of some biogenic amines via RP-HPLC using mixed micellar mobile phase was investigated. The compounds were derivatized before hand by dansyl chloride as a chromophoric reagent. Appropriate conditions for separation, were determined by studying factors such as temperature, type and percentage of organic modifier, concentration of surfactants (SDS and Brij-35) and the pH of the mobile phase. The number of theoretical plates (N), asymmetry factor (B/A) and selectivity factor (α) considered as criteria for finding the appropriate conditions for separation. Appropriate condition, were: 40˚C, 40 mM SDS, 0.5 mM Brij-35 and 10% 1-propanol at pH=5.