High throughput automated colorimetric method for the screening of l-lactic acid producing microorganisms
Lactic acid is a valuable and fully degradable organic acid with promising applications in poly-lactic acid production (Taskila S and Ojamo, 2013 [1]). Despite their efficiency, the cost of the current lactic acid bio-processes is still an obstacle to this application (Miller et al., 2011 [2]). To a...
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doaj-446916858ce94fbc84874a7b0ef0fd3a2020-11-24T21:44:14ZengElsevierMethodsX2215-01612014-01-011C25425710.1016/j.mex.2014.10.001High throughput automated colorimetric method for the screening of l-lactic acid producing microorganismsNadège Liaud0David Navarro1Nicolas Vidal2Jean-Claude Sigoillot3Sana Raouche4INRA, UMR1163 Biotechnology and Biodiversity of Fungi, F-13288 Marseille, FranceINRA, UMR1163 Biotechnology and Biodiversity of Fungi, F-13288 Marseille, FranceYelen, 10 boulevard Tempête, F-13820 Ensues la redonne, FranceINRA, UMR1163 Biotechnology and Biodiversity of Fungi, F-13288 Marseille, FranceINRA, UMR1163 Biotechnology and Biodiversity of Fungi, F-13288 Marseille, FranceLactic acid is a valuable and fully degradable organic acid with promising applications in poly-lactic acid production (Taskila S and Ojamo, 2013 [1]). Despite their efficiency, the cost of the current lactic acid bio-processes is still an obstacle to this application (Miller et al., 2011 [2]). To ameliorate lactic acid producing strains, researchers are using mutations and metabolic engineering techniques, as well as medium optimization. All these studies necessitate a good and high throughput screening method. Currently, researchers mostly use HPLC methods which often necessitate sample preparation, are not stereospecific and do not allow high throughput. To help optimizing l-lactic acid production, we developed a high throughput colorimetric method inspired by the blood l-lactic acid detection method used for diagnosis (Lin et al., 1999 [3]). • Two sequential enzymatic reactions using l-lactate oxidase, peroxidase and ABTS (2,2′-azino-di-[3-ethylbenzthiazoine-sulfonate]), a chromogenic peroxidase substrate, are used to quantify l-lactate between 13.8 and 90 mg/l. • The accuracy of the method was ascertained before automation. • The method was successfully applied for the direct determination of l-lactate content in fungal culture supernatants.http://www.sciencedirect.com/science/article/pii/S2215016114000156Automated screening for lactic acid production |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nadège Liaud David Navarro Nicolas Vidal Jean-Claude Sigoillot Sana Raouche |
spellingShingle |
Nadège Liaud David Navarro Nicolas Vidal Jean-Claude Sigoillot Sana Raouche High throughput automated colorimetric method for the screening of l-lactic acid producing microorganisms MethodsX Automated screening for lactic acid production |
author_facet |
Nadège Liaud David Navarro Nicolas Vidal Jean-Claude Sigoillot Sana Raouche |
author_sort |
Nadège Liaud |
title |
High throughput automated colorimetric method for the screening of l-lactic acid producing microorganisms |
title_short |
High throughput automated colorimetric method for the screening of l-lactic acid producing microorganisms |
title_full |
High throughput automated colorimetric method for the screening of l-lactic acid producing microorganisms |
title_fullStr |
High throughput automated colorimetric method for the screening of l-lactic acid producing microorganisms |
title_full_unstemmed |
High throughput automated colorimetric method for the screening of l-lactic acid producing microorganisms |
title_sort |
high throughput automated colorimetric method for the screening of l-lactic acid producing microorganisms |
publisher |
Elsevier |
series |
MethodsX |
issn |
2215-0161 |
publishDate |
2014-01-01 |
description |
Lactic acid is a valuable and fully degradable organic acid with promising applications in poly-lactic acid production (Taskila S and Ojamo, 2013 [1]). Despite their efficiency, the cost of the current lactic acid bio-processes is still an obstacle to this application (Miller et al., 2011 [2]). To ameliorate lactic acid producing strains, researchers are using mutations and metabolic engineering techniques, as well as medium optimization. All these studies necessitate a good and high throughput screening method. Currently, researchers mostly use HPLC methods which often necessitate sample preparation, are not stereospecific and do not allow high throughput. To help optimizing l-lactic acid production, we developed a high throughput colorimetric method inspired by the blood l-lactic acid detection method used for diagnosis (Lin et al., 1999 [3]).
•
Two sequential enzymatic reactions using l-lactate oxidase, peroxidase and ABTS (2,2′-azino-di-[3-ethylbenzthiazoine-sulfonate]), a chromogenic peroxidase substrate, are used to quantify l-lactate between 13.8 and 90 mg/l.
•
The accuracy of the method was ascertained before automation.
•
The method was successfully applied for the direct determination of l-lactate content in fungal culture supernatants. |
topic |
Automated screening for lactic acid production |
url |
http://www.sciencedirect.com/science/article/pii/S2215016114000156 |
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