Caloric Restriction in Group-Housed Mice: Littermate and Sex Influence on Behavioral and Hormonal Data
Much of the research done on aging, oxidative stress, anxiety, and cognitive and social behavior in rodents has focused on caloric restriction (CR). This often involves several days of single housing, which can cause numerous logistical problems, as well as cognitive and social dysfunctions. Previou...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2021-04-01
|
Series: | Frontiers in Veterinary Science |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.639187/full |
id |
doaj-44773b95392449aaa56a9ef17f0a56b2 |
---|---|
record_format |
Article |
spelling |
doaj-44773b95392449aaa56a9ef17f0a56b22021-04-15T04:31:33ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-04-01810.3389/fvets.2021.639187639187Caloric Restriction in Group-Housed Mice: Littermate and Sex Influence on Behavioral and Hormonal DataCristina Perea0Ana Vázquez-Ágredos1Leandro Ruiz-Leyva2Leandro Ruiz-Leyva3Ignacio Morón4Jesús Martín Zúñiga5Cruz Miguel Cendán6Cruz Miguel Cendán7Center of Scientific Instrumentation, University of Granada, Granada, SpainDepartment of Psychobiology, Institute of Neurosciences, Center for Biomedical Research (CIBM), University of Granada, Granada, SpainDepartment of Pharmacology, Faculty of Medicine, Biomedical Research Center, Institute of Neuroscience, University of Granada, Parque Tecnológico de Ciencias de la Salud, Granada, SpainBiosanitary Research Institute ibs.GRANADA, Granada, SpainDepartment of Psychobiology, Faculty of Psychology, Center of Investigation of Mind, Brain, and Behavior, University of Granada, Granada, SpainCenter of Scientific Instrumentation, University of Granada, Granada, SpainDepartment of Pharmacology, Faculty of Medicine, Biomedical Research Center, Institute of Neuroscience, University of Granada, Parque Tecnológico de Ciencias de la Salud, Granada, SpainBiosanitary Research Institute ibs.GRANADA, Granada, SpainMuch of the research done on aging, oxidative stress, anxiety, and cognitive and social behavior in rodents has focused on caloric restriction (CR). This often involves several days of single housing, which can cause numerous logistical problems, as well as cognitive and social dysfunctions. Previous results in our laboratory showed the viability of long-term CR in grouped rats. Our research has studied the possibility of CR in grouped female and male littermates and unrelated CB6F1/J (C57BL/6J × BALBc/J hybrid strain) mice, measuring: (i) possible differences in body mass proportions between mice in ad libitum and CR conditions (at 70% of ad libitum), (ii) aggressive behavior, using the number of pushes and chasing behavior time as an indicator and social behavior using the time under the feeder as indicator, and (iii) difference in serum adrenocorticotropic hormone (ACTH) concentrations (stress biomarker), under ad libitum and CR conditions. Results showed the impossibility of implementing CR in unrelated male mice. In all other groups, CR was possible, with a less aggressive behavior (measured only with the number of pushes) observed in the unrelated female mice under CR conditions. In that sense, the ACTH levels measured on the last day of CR showed no difference in stress levels. These results indicate that implementantion of long-term CR in mice can be optimized technically and also related to their well-being by grouping animals, in particular, related mice.https://www.frontiersin.org/articles/10.3389/fvets.2021.639187/fullcaloric restrictiongrouped micelittermate miceadrenocorticotropiceating behaviorsocial behavior |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cristina Perea Ana Vázquez-Ágredos Leandro Ruiz-Leyva Leandro Ruiz-Leyva Ignacio Morón Jesús Martín Zúñiga Cruz Miguel Cendán Cruz Miguel Cendán |
spellingShingle |
Cristina Perea Ana Vázquez-Ágredos Leandro Ruiz-Leyva Leandro Ruiz-Leyva Ignacio Morón Jesús Martín Zúñiga Cruz Miguel Cendán Cruz Miguel Cendán Caloric Restriction in Group-Housed Mice: Littermate and Sex Influence on Behavioral and Hormonal Data Frontiers in Veterinary Science caloric restriction grouped mice littermate mice adrenocorticotropic eating behavior social behavior |
author_facet |
Cristina Perea Ana Vázquez-Ágredos Leandro Ruiz-Leyva Leandro Ruiz-Leyva Ignacio Morón Jesús Martín Zúñiga Cruz Miguel Cendán Cruz Miguel Cendán |
author_sort |
Cristina Perea |
title |
Caloric Restriction in Group-Housed Mice: Littermate and Sex Influence on Behavioral and Hormonal Data |
title_short |
Caloric Restriction in Group-Housed Mice: Littermate and Sex Influence on Behavioral and Hormonal Data |
title_full |
Caloric Restriction in Group-Housed Mice: Littermate and Sex Influence on Behavioral and Hormonal Data |
title_fullStr |
Caloric Restriction in Group-Housed Mice: Littermate and Sex Influence on Behavioral and Hormonal Data |
title_full_unstemmed |
Caloric Restriction in Group-Housed Mice: Littermate and Sex Influence on Behavioral and Hormonal Data |
title_sort |
caloric restriction in group-housed mice: littermate and sex influence on behavioral and hormonal data |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Veterinary Science |
issn |
2297-1769 |
publishDate |
2021-04-01 |
description |
Much of the research done on aging, oxidative stress, anxiety, and cognitive and social behavior in rodents has focused on caloric restriction (CR). This often involves several days of single housing, which can cause numerous logistical problems, as well as cognitive and social dysfunctions. Previous results in our laboratory showed the viability of long-term CR in grouped rats. Our research has studied the possibility of CR in grouped female and male littermates and unrelated CB6F1/J (C57BL/6J × BALBc/J hybrid strain) mice, measuring: (i) possible differences in body mass proportions between mice in ad libitum and CR conditions (at 70% of ad libitum), (ii) aggressive behavior, using the number of pushes and chasing behavior time as an indicator and social behavior using the time under the feeder as indicator, and (iii) difference in serum adrenocorticotropic hormone (ACTH) concentrations (stress biomarker), under ad libitum and CR conditions. Results showed the impossibility of implementing CR in unrelated male mice. In all other groups, CR was possible, with a less aggressive behavior (measured only with the number of pushes) observed in the unrelated female mice under CR conditions. In that sense, the ACTH levels measured on the last day of CR showed no difference in stress levels. These results indicate that implementantion of long-term CR in mice can be optimized technically and also related to their well-being by grouping animals, in particular, related mice. |
topic |
caloric restriction grouped mice littermate mice adrenocorticotropic eating behavior social behavior |
url |
https://www.frontiersin.org/articles/10.3389/fvets.2021.639187/full |
work_keys_str_mv |
AT cristinaperea caloricrestrictioningrouphousedmicelittermateandsexinfluenceonbehavioralandhormonaldata AT anavazquezagredos caloricrestrictioningrouphousedmicelittermateandsexinfluenceonbehavioralandhormonaldata AT leandroruizleyva caloricrestrictioningrouphousedmicelittermateandsexinfluenceonbehavioralandhormonaldata AT leandroruizleyva caloricrestrictioningrouphousedmicelittermateandsexinfluenceonbehavioralandhormonaldata AT ignaciomoron caloricrestrictioningrouphousedmicelittermateandsexinfluenceonbehavioralandhormonaldata AT jesusmartinzuniga caloricrestrictioningrouphousedmicelittermateandsexinfluenceonbehavioralandhormonaldata AT cruzmiguelcendan caloricrestrictioningrouphousedmicelittermateandsexinfluenceonbehavioralandhormonaldata AT cruzmiguelcendan caloricrestrictioningrouphousedmicelittermateandsexinfluenceonbehavioralandhormonaldata |
_version_ |
1721526635331584000 |