Evaluation of an Integrated Ultrafiltration/Solid Phase Extraction Process for Purification of Oligomeric Grape Seed Procyanidins
The effectiveness of a preparative integrated ultrafiltration/solid-phase extraction (UF/SPE) process for purification of oligomeric procyanidins (OPCs) from a crude grape seed extract (GSE) was studied for the first time. The separation of OPCs from polymeric procyanidins (PPCs) by UF was very effi...
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doaj-447834032825470fbd6ae8cc4e80d4b12020-11-25T02:59:54ZengMDPI AGMembranes2077-03752020-07-011014714710.3390/membranes10070147Evaluation of an Integrated Ultrafiltration/Solid Phase Extraction Process for Purification of Oligomeric Grape Seed ProcyanidinsAlba Gutierrez-Docio0Paula Almodóvar1Silvia Moreno-Fernandez2Jose Manuel Silvan3Adolfo J. Martinez-Rodriguez4Gonzalo Luis Alonso5Marin Prodanov6Functional Food Ingredients Group, Department of Production and Characterization of Novel Foods, Instituto de Investigación en Ciencias de la Alimentación (CIAL), (CEI, CSIC-UAM), C/ Nicolás Cabrera, 9. Campus Cantoblanco, Universidad Autónoma de Madrid, 28049 Madrid, SpainFunctional Food Ingredients Group, Department of Production and Characterization of Novel Foods, Instituto de Investigación en Ciencias de la Alimentación (CIAL), (CEI, CSIC-UAM), C/ Nicolás Cabrera, 9. Campus Cantoblanco, Universidad Autónoma de Madrid, 28049 Madrid, SpainFunctional Food Ingredients Group, Department of Production and Characterization of Novel Foods, Instituto de Investigación en Ciencias de la Alimentación (CIAL), (CEI, CSIC-UAM), C/ Nicolás Cabrera, 9. Campus Cantoblanco, Universidad Autónoma de Madrid, 28049 Madrid, SpainMicrobiology and Food Biocatalysis Group, Department of Biotechnology and Food Microbiology, Instituto de Investigación en Ciencias de la Alimentación (CIAL) (CEI, CSIC-UAM), C/ Nicolás Cabrera, 9. Campus Cantoblanco, Universidad Autónoma de Madrid, 28049 Madrid, SpainMicrobiology and Food Biocatalysis Group, Department of Biotechnology and Food Microbiology, Instituto de Investigación en Ciencias de la Alimentación (CIAL) (CEI, CSIC-UAM), C/ Nicolás Cabrera, 9. Campus Cantoblanco, Universidad Autónoma de Madrid, 28049 Madrid, SpainCátedra de Química Agrícola, E.T.S.I. Agrónomos y Montes, Universidad de Castilla-La Mancha, Avda. de España s/n, 02071 Albacete, SpainFunctional Food Ingredients Group, Department of Production and Characterization of Novel Foods, Instituto de Investigación en Ciencias de la Alimentación (CIAL), (CEI, CSIC-UAM), C/ Nicolás Cabrera, 9. Campus Cantoblanco, Universidad Autónoma de Madrid, 28049 Madrid, SpainThe effectiveness of a preparative integrated ultrafiltration/solid-phase extraction (UF/SPE) process for purification of oligomeric procyanidins (OPCs) from a crude grape seed extract (GSE) was studied for the first time. The separation of OPCs from polymeric procyanidins (PPCs) by UF was very efficient. The membrane showed an acceptable filtration flux of 6 to 3.5 L/h·m<sup>2</sup> at 0.5 bar of transmembrane pressure and 95% recovery of its water flux after chemical cleaning. The process was scalable to a pilot scale. The separation of very polar and ionic species from OPCs by SPE (XAD7HP and XAD16 resins) was also very good, but both adsorbents lost their retention capacities quickly, due probably to irreversible retention of OPCs/PPCs. Even though the global purification of OPCs by the integrated UF/SPE process allowed the recovery of 24.2 g of highly purified OPCs (83% purity) from 14.4 L of crude grape seed extract, the use of these adsorbents for further purification of the OPCs was very limited.https://www.mdpi.com/2077-0375/10/7/147grape seed extractpurificationtangential-flow pressure-driven membrane ultrafiltrationsolid-phase extractionoligomeric procyanidins |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alba Gutierrez-Docio Paula Almodóvar Silvia Moreno-Fernandez Jose Manuel Silvan Adolfo J. Martinez-Rodriguez Gonzalo Luis Alonso Marin Prodanov |
spellingShingle |
Alba Gutierrez-Docio Paula Almodóvar Silvia Moreno-Fernandez Jose Manuel Silvan Adolfo J. Martinez-Rodriguez Gonzalo Luis Alonso Marin Prodanov Evaluation of an Integrated Ultrafiltration/Solid Phase Extraction Process for Purification of Oligomeric Grape Seed Procyanidins Membranes grape seed extract purification tangential-flow pressure-driven membrane ultrafiltration solid-phase extraction oligomeric procyanidins |
author_facet |
Alba Gutierrez-Docio Paula Almodóvar Silvia Moreno-Fernandez Jose Manuel Silvan Adolfo J. Martinez-Rodriguez Gonzalo Luis Alonso Marin Prodanov |
author_sort |
Alba Gutierrez-Docio |
title |
Evaluation of an Integrated Ultrafiltration/Solid Phase Extraction Process for Purification of Oligomeric Grape Seed Procyanidins |
title_short |
Evaluation of an Integrated Ultrafiltration/Solid Phase Extraction Process for Purification of Oligomeric Grape Seed Procyanidins |
title_full |
Evaluation of an Integrated Ultrafiltration/Solid Phase Extraction Process for Purification of Oligomeric Grape Seed Procyanidins |
title_fullStr |
Evaluation of an Integrated Ultrafiltration/Solid Phase Extraction Process for Purification of Oligomeric Grape Seed Procyanidins |
title_full_unstemmed |
Evaluation of an Integrated Ultrafiltration/Solid Phase Extraction Process for Purification of Oligomeric Grape Seed Procyanidins |
title_sort |
evaluation of an integrated ultrafiltration/solid phase extraction process for purification of oligomeric grape seed procyanidins |
publisher |
MDPI AG |
series |
Membranes |
issn |
2077-0375 |
publishDate |
2020-07-01 |
description |
The effectiveness of a preparative integrated ultrafiltration/solid-phase extraction (UF/SPE) process for purification of oligomeric procyanidins (OPCs) from a crude grape seed extract (GSE) was studied for the first time. The separation of OPCs from polymeric procyanidins (PPCs) by UF was very efficient. The membrane showed an acceptable filtration flux of 6 to 3.5 L/h·m<sup>2</sup> at 0.5 bar of transmembrane pressure and 95% recovery of its water flux after chemical cleaning. The process was scalable to a pilot scale. The separation of very polar and ionic species from OPCs by SPE (XAD7HP and XAD16 resins) was also very good, but both adsorbents lost their retention capacities quickly, due probably to irreversible retention of OPCs/PPCs. Even though the global purification of OPCs by the integrated UF/SPE process allowed the recovery of 24.2 g of highly purified OPCs (83% purity) from 14.4 L of crude grape seed extract, the use of these adsorbents for further purification of the OPCs was very limited. |
topic |
grape seed extract purification tangential-flow pressure-driven membrane ultrafiltration solid-phase extraction oligomeric procyanidins |
url |
https://www.mdpi.com/2077-0375/10/7/147 |
work_keys_str_mv |
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