A human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl esters
Acyl-CoA-dependent O-acyltransferases catalyze reactions in which fatty acyl-CoAs are joined to acyl acceptors containing free hydroxyl groups to produce neutral lipids. In this report, we characterize a human multifunctional O-acyltransferase (designated MFAT) that belongs to the acyl-CoA:diacylgly...
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doaj-448609ea5dec4569a44913c37062d9f22021-04-27T04:43:28ZengElsevierJournal of Lipid Research0022-22752005-11-01461123882397A human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl estersChi-Liang Eric Yen0Charles H. Brown, IV1Mara Monetti2Robert V. Farese, Jr.3To whom correspondence should be addressed.; Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158; Cardiovascular Research Institute, University of California, San Francisco, CA 94143Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158; Cardiovascular Research Institute, University of California, San Francisco, CA 94143Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158; Cardiovascular Research Institute, University of California, San Francisco, CA 94143; Department of Medicine, University of California, San Francisco, CA 94143Acyl-CoA-dependent O-acyltransferases catalyze reactions in which fatty acyl-CoAs are joined to acyl acceptors containing free hydroxyl groups to produce neutral lipids. In this report, we characterize a human multifunctional O-acyltransferase (designated MFAT) that belongs to the acyl-CoA:diacylglycerol acyltransferase 2/acyl-CoA:monoacylglycerol acyltransferase (MGAT) gene family and is highly expressed in the skin. Membranes of insect cells and homogenates of mammalian cells overexpressing MFAT exhibited significantly increased MGAT, acyl-CoA:fatty acyl alcohol acyltransferase (wax synthase), and acyl-CoA:retinol acyltransferase (ARAT) activities, which catalyze the synthesis of diacylglycerols, wax monoesters, and retinyl esters, respectively. Furthermore, when provided with the appropriate substrates, intact mammalian cells overexpressing MFAT accumulated more waxes and retinyl esters than control cells.We conclude that MFAT is a multifunctional acyltransferase that likely plays an important role in lipid metabolism in human skin.http://www.sciencedirect.com/science/article/pii/S0022227520328765neutral lipidsdiacylglycerolfatty alcoholesterification |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chi-Liang Eric Yen Charles H. Brown, IV Mara Monetti Robert V. Farese, Jr. |
spellingShingle |
Chi-Liang Eric Yen Charles H. Brown, IV Mara Monetti Robert V. Farese, Jr. A human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl esters Journal of Lipid Research neutral lipids diacylglycerol fatty alcohol esterification |
author_facet |
Chi-Liang Eric Yen Charles H. Brown, IV Mara Monetti Robert V. Farese, Jr. |
author_sort |
Chi-Liang Eric Yen |
title |
A human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl esters |
title_short |
A human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl esters |
title_full |
A human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl esters |
title_fullStr |
A human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl esters |
title_full_unstemmed |
A human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl esters |
title_sort |
human skin multifunctional o-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl esters |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2005-11-01 |
description |
Acyl-CoA-dependent O-acyltransferases catalyze reactions in which fatty acyl-CoAs are joined to acyl acceptors containing free hydroxyl groups to produce neutral lipids. In this report, we characterize a human multifunctional O-acyltransferase (designated MFAT) that belongs to the acyl-CoA:diacylglycerol acyltransferase 2/acyl-CoA:monoacylglycerol acyltransferase (MGAT) gene family and is highly expressed in the skin. Membranes of insect cells and homogenates of mammalian cells overexpressing MFAT exhibited significantly increased MGAT, acyl-CoA:fatty acyl alcohol acyltransferase (wax synthase), and acyl-CoA:retinol acyltransferase (ARAT) activities, which catalyze the synthesis of diacylglycerols, wax monoesters, and retinyl esters, respectively. Furthermore, when provided with the appropriate substrates, intact mammalian cells overexpressing MFAT accumulated more waxes and retinyl esters than control cells.We conclude that MFAT is a multifunctional acyltransferase that likely plays an important role in lipid metabolism in human skin. |
topic |
neutral lipids diacylglycerol fatty alcohol esterification |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520328765 |
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