Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time

Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time

The circadian clock proteins KaiA, KaiB, and KaiC reconstitute a circa-24 h oscillation of KaiC phosphorylation in vitro. Here the authors use high-speed atomic force microscopy to visualize in real time and quantify the dynamic interactions of KaiA with KaiC on the sub-second timescale to discover...

Full description

Bibliographic Details
Main Authors: Tetsuya Mori, Shogo Sugiyama, Mark Byrne, Carl Hirschie Johnson, Takayuki Uchihashi, Toshio Ando
Format: Article
Language:English
Published: Nature Publishing Group 2018-08-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-05438-4
id doaj-449004dac2544cb1b23b08abbadf63e7
record_format Article
spelling doaj-449004dac2544cb1b23b08abbadf63e72021-05-11T10:12:54ZengNature Publishing GroupNature Communications2041-17232018-08-019111310.1038/s41467-018-05438-4Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real timeTetsuya Mori0Shogo Sugiyama1Mark Byrne2Carl Hirschie Johnson3Takayuki Uchihashi4Toshio Ando5Department of Biological Sciences, Vanderbilt UniversityDepartment of Physics, College of Science and Engineering, Kanazawa UniversityDepartment of Chemistry, Physics, and EngineeringDepartment of Biological Sciences, Vanderbilt UniversityDepartment of Physics and Structural Biology Research Center, Nagoya UniversityNano Life Science Institute (WPI-NanoLSI), Kanazawa UniversityThe circadian clock proteins KaiA, KaiB, and KaiC reconstitute a circa-24 h oscillation of KaiC phosphorylation in vitro. Here the authors use high-speed atomic force microscopy to visualize in real time and quantify the dynamic interactions of KaiA with KaiC on the sub-second timescale to discover mechanisms of oscillatory resilience.https://doi.org/10.1038/s41467-018-05438-4
collection DOAJ
language English
format Article
sources DOAJ
author Tetsuya Mori
Shogo Sugiyama
Mark Byrne
Carl Hirschie Johnson
Takayuki Uchihashi
Toshio Ando
spellingShingle Tetsuya Mori
Shogo Sugiyama
Mark Byrne
Carl Hirschie Johnson
Takayuki Uchihashi
Toshio Ando
Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time
Nature Communications
author_facet Tetsuya Mori
Shogo Sugiyama
Mark Byrne
Carl Hirschie Johnson
Takayuki Uchihashi
Toshio Ando
author_sort Tetsuya Mori
title Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time
title_short Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time
title_full Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time
title_fullStr Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time
title_full_unstemmed Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time
title_sort revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2018-08-01
description The circadian clock proteins KaiA, KaiB, and KaiC reconstitute a circa-24 h oscillation of KaiC phosphorylation in vitro. Here the authors use high-speed atomic force microscopy to visualize in real time and quantify the dynamic interactions of KaiA with KaiC on the sub-second timescale to discover mechanisms of oscillatory resilience.
url https://doi.org/10.1038/s41467-018-05438-4
work_keys_str_mv AT tetsuyamori revealingcircadianmechanismsofintegrationandresiliencebyvisualizingclockproteinsworkinginrealtime
AT shogosugiyama revealingcircadianmechanismsofintegrationandresiliencebyvisualizingclockproteinsworkinginrealtime
AT markbyrne revealingcircadianmechanismsofintegrationandresiliencebyvisualizingclockproteinsworkinginrealtime
AT carlhirschiejohnson revealingcircadianmechanismsofintegrationandresiliencebyvisualizingclockproteinsworkinginrealtime
AT takayukiuchihashi revealingcircadianmechanismsofintegrationandresiliencebyvisualizingclockproteinsworkinginrealtime
AT toshioando revealingcircadianmechanismsofintegrationandresiliencebyvisualizingclockproteinsworkinginrealtime
_version_ 1721448493192577024

Similar Items