The Influence of Processing Parameters on the Mitigation of Deoxynivalenol during Industrial Baking

• Main Authors: David Stadler, Francesca Lambertini, Lydia Woelflingseder, Heidi Schwartz-Zimmermann, Doris Marko, Michele Suman, Franz Berthiller, Rudolf Krska
• Format: Article
• Language: English
• Published: MDPI AG 2019-06-01
• Series: Toxins
• Subjects: mycotoxins; trichothecenes; thermal degradation; decontamination; mass spectrometry; food processing; detoxification; design of experiment; LC-MS/MS
• Online Access: https://www.mdpi.com/2072-6651/11/6/317

Deoxynivalenol (DON), a frequent contaminant of flour, can be partially degraded by baking. It is not clear: (i) How the choice of processing parameter (i.e., ingredients, leavening, and baking conditions) affects DON degradation and thus (ii) how much DON can be degraded during the large-scale industrial production of bakery products. Crackers, biscuits, and bread were produced from naturally contaminated flour using different processing conditions. DON degradation during baking was quantified with the most accurate analytical methodology available for this Fusarium toxin, which is based on liquid chromatography tandem mass spectrometry. Depending on the processing conditions, 0−21%, 4−16%, and 2−5% DON were degraded during the production of crackers, biscuits, and bread, respectively. A higher NaHCO₃ concentration, baking time, and baking temperature caused higher DON degradation. NH₄HCO₃, yeast, vinegar, and sucrose concentration as well as leavening time did not enhance DON degradation. In vitro cell viability assays confirmed that the major degradation product isoDON is considerably less toxic than DON. This proves for the first time that large-scale industrial baking results in partial detoxification of DON, which can be enhanced by process management.