Edaravone combined with Schwann cell transplantation may repair spinal cord injury in rats

• Main Authors: Shu-quan Zhang, Min-fei Wu, Zhe Piao, Jin Yao, Ji-hai Li, Xin-gang Wang, Jun Liu
• Format: Article
• Language: English
• Published: Wolters Kluwer Medknow Publications 2015-01-01
• Series: Neural Regeneration Research
• Subjects: Alzheimer′s disease; amyloid-β; astrocytes; Ca 2+; calcilytic; calcium-sensing receptor; nitromemantine; NPS 2143; α7-nicotinic acetylcholine receptor; nerve regeneration; spinal cord injury; surgical decompression; tumor necrosis factor α; cell apoptosis; neurological function; neural regeneration; contusion; Nogo-A; axon growth; immunohistochemistry; fluorescent quantitative PCR; Schwann cells; cell transplantation; edaravone; motor function; electrophysiological function
• Online Access: http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=2;spage=230;epage=236;aulast=Zhang

Edaravone has been shown to delay neuronal apoptosis, thereby improving nerve function and the microenvironment after spinal cord injury. Edaravone can provide a favorable environment for the treatment of spinal cord injury using Schwann cell transplantation. This study used rat models of complete spinal cord transection at T 9. Six hours later, Schwann cells were transplanted in the head and tail ends of the injury site. Simultaneously, edaravone was injected through the caudal vein. Eight weeks later, the PKH-26-labeled Schwann cells had survived and migrated to the center of the spinal cord injury region in rats after combined treatment with edaravone and Schwann cells. Moreover, the number of PKH-26-labeled Schwann cells in the rat spinal cord was more than that in rats undergoing Schwann cell transplantation alone or rats without any treatment. Horseradish peroxidase retrograde tracing revealed that the number of horseradish peroxidase-positive nerve fibers was greater in rats treated with edaravone combined withSchwann cells than in rats with Schwann cell transplantation alone. The results demonstrated that lower extremity motor function and neurophysiological function were better in rats treated with edaravone and Schwann cells than in rats with Schwann cell transplantation only. These data confirmed that Schwann cell transplantation combined with edaravone injection promoted the regeneration of nerve fibers of rats with spinal cord injury and improved neurological function.