Antifungal Activity in Compounds from the Australian Desert Plant <i>Eremophila alternifolia</i> with Potency Against <i>Cryptococcus spp.</i>
Plant metabolites that have shown activity against bacteria and/or environmental fungi represent valuable leads for the identification and development of novel drugs against clinically important human pathogenic fungi. Plants from the genus <i>Eremophila</i> were highly valued in traditi...
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doaj-44b75aa538014e4e854b891316bd96ce2020-11-24T21:49:07ZengMDPI AGAntibiotics2079-63822019-03-01823410.3390/antibiotics8020034antibiotics8020034Antifungal Activity in Compounds from the Australian Desert Plant <i>Eremophila alternifolia</i> with Potency Against <i>Cryptococcus spp.</i>Mohammed A. Hossain0Israt J. Biva1Sarah E. Kidd2Jason D. Whittle3Hans J. Griesser4Bryan R. Coad5Future Industries Institute, University of South Australia, Mawson Lakes, South Australia 5095, AustraliaFuture Industries Institute, University of South Australia, Mawson Lakes, South Australia 5095, AustraliaNational Mycology Reference Centre, SA Pathology, Adelaide, South Australia 5000, AustraliaSchool of Engineering, University of South Australia, Mawson Lakes, South Australia 5095, AustraliaFuture Industries Institute, University of South Australia, Mawson Lakes, South Australia 5095, AustraliaFuture Industries Institute, University of South Australia, Mawson Lakes, South Australia 5095, AustraliaPlant metabolites that have shown activity against bacteria and/or environmental fungi represent valuable leads for the identification and development of novel drugs against clinically important human pathogenic fungi. Plants from the genus <i>Eremophila</i> were highly valued in traditional Australian Aboriginal medicinal practices, and <i>E. alternifolia</i> was the most prized among them. As antibacterial activity of extracts from <i>E. alternifolia</i> has been documented, this study addresses the question whether there is also activity against infectious fungal human pathogens. Compounds from leaf-extracts were purified and identified by 1- and 2-D NMR. These were then tested by disk diffusion and broth microdilution assays against ten clinically and environmentally relevant yeast and mould species. The most potent activity was observed with the diterpene compound, 8,19-dihydroxyserrulat-14-ene against <i>Cryptococcus gattii</i> and <i>Cryptococcus neoformans</i>, with minimum inhibition concentrations (MIC) comparable to those of Amphotericin B. This compound also exhibited activity against six <i>Candida</i> species. Combined with previous studies showing an antibacterial effect, this finding could explain a broad antimicrobial effect from <i>Eremophila</i> extracts in their traditional medicinal usage. The discovery of potent antifungal compounds from <i>Eremophila</i> extracts is a promising development in the search for desperately needed antifungal compounds particularly for <i>Cryptococcus</i> infections.https://www.mdpi.com/2079-6382/8/2/34Diterpenoidsantifungalwound healingserrulatanefungi<i>Eremophila</i><i>Cryptococcus</i>disk diffusionbroth microdilution |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohammed A. Hossain Israt J. Biva Sarah E. Kidd Jason D. Whittle Hans J. Griesser Bryan R. Coad |
spellingShingle |
Mohammed A. Hossain Israt J. Biva Sarah E. Kidd Jason D. Whittle Hans J. Griesser Bryan R. Coad Antifungal Activity in Compounds from the Australian Desert Plant <i>Eremophila alternifolia</i> with Potency Against <i>Cryptococcus spp.</i> Antibiotics Diterpenoids antifungal wound healing serrulatane fungi <i>Eremophila</i> <i>Cryptococcus</i> disk diffusion broth microdilution |
author_facet |
Mohammed A. Hossain Israt J. Biva Sarah E. Kidd Jason D. Whittle Hans J. Griesser Bryan R. Coad |
author_sort |
Mohammed A. Hossain |
title |
Antifungal Activity in Compounds from the Australian Desert Plant <i>Eremophila alternifolia</i> with Potency Against <i>Cryptococcus spp.</i> |
title_short |
Antifungal Activity in Compounds from the Australian Desert Plant <i>Eremophila alternifolia</i> with Potency Against <i>Cryptococcus spp.</i> |
title_full |
Antifungal Activity in Compounds from the Australian Desert Plant <i>Eremophila alternifolia</i> with Potency Against <i>Cryptococcus spp.</i> |
title_fullStr |
Antifungal Activity in Compounds from the Australian Desert Plant <i>Eremophila alternifolia</i> with Potency Against <i>Cryptococcus spp.</i> |
title_full_unstemmed |
Antifungal Activity in Compounds from the Australian Desert Plant <i>Eremophila alternifolia</i> with Potency Against <i>Cryptococcus spp.</i> |
title_sort |
antifungal activity in compounds from the australian desert plant <i>eremophila alternifolia</i> with potency against <i>cryptococcus spp.</i> |
publisher |
MDPI AG |
series |
Antibiotics |
issn |
2079-6382 |
publishDate |
2019-03-01 |
description |
Plant metabolites that have shown activity against bacteria and/or environmental fungi represent valuable leads for the identification and development of novel drugs against clinically important human pathogenic fungi. Plants from the genus <i>Eremophila</i> were highly valued in traditional Australian Aboriginal medicinal practices, and <i>E. alternifolia</i> was the most prized among them. As antibacterial activity of extracts from <i>E. alternifolia</i> has been documented, this study addresses the question whether there is also activity against infectious fungal human pathogens. Compounds from leaf-extracts were purified and identified by 1- and 2-D NMR. These were then tested by disk diffusion and broth microdilution assays against ten clinically and environmentally relevant yeast and mould species. The most potent activity was observed with the diterpene compound, 8,19-dihydroxyserrulat-14-ene against <i>Cryptococcus gattii</i> and <i>Cryptococcus neoformans</i>, with minimum inhibition concentrations (MIC) comparable to those of Amphotericin B. This compound also exhibited activity against six <i>Candida</i> species. Combined with previous studies showing an antibacterial effect, this finding could explain a broad antimicrobial effect from <i>Eremophila</i> extracts in their traditional medicinal usage. The discovery of potent antifungal compounds from <i>Eremophila</i> extracts is a promising development in the search for desperately needed antifungal compounds particularly for <i>Cryptococcus</i> infections. |
topic |
Diterpenoids antifungal wound healing serrulatane fungi <i>Eremophila</i> <i>Cryptococcus</i> disk diffusion broth microdilution |
url |
https://www.mdpi.com/2079-6382/8/2/34 |
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