Whole exome sequencing of lung adenocarcinoma and lung squamous cell carcinoma in one individual: A case report

Multiple primary lung cancers (MPLCs) refers to two or more primary malignant tumors that occur simultaneously or successively in the lung of the same patient. Distinguishing MPLCs from intrapulmonary metastases is important for treatment strategy and prognosis. MPLCs have been considered as having...

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Main Authors: Yuxi Bai, Yinghui Xu, Xu Wang, Yunpeng Liu, Chao Sun, Ye Guo, Yangyang Cai, Guoguang Shao, Zhiguang Yang, Shi Qiu, Kewei Ma
Format: Article
Language:English
Published: Wiley 2020-08-01
Series:Thoracic Cancer
Subjects:
Online Access:https://doi.org/10.1111/1759-7714.13540
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spelling doaj-44bafcd6ed274b7b814787f5c89a30c12020-11-25T02:50:01ZengWileyThoracic Cancer1759-77061759-77142020-08-011182361236410.1111/1759-7714.13540Whole exome sequencing of lung adenocarcinoma and lung squamous cell carcinoma in one individual: A case reportYuxi Bai0Yinghui Xu1Xu Wang2Yunpeng Liu3Chao Sun4Ye Guo5Yangyang Cai6Guoguang Shao7Zhiguang Yang8Shi Qiu9Kewei Ma10Cancer Center The First Hospital of Jilin University Jilin ChinaCancer Center The First Hospital of Jilin University Jilin ChinaCancer Center The First Hospital of Jilin University Jilin ChinaThoracic Surgery Department The First Hospital of Jilin University Jilin ChinaCancer Center The First Hospital of Jilin University Jilin ChinaCancer Center The First Hospital of Jilin University Jilin ChinaCancer Center The First Hospital of Jilin University Jilin ChinaThoracic Surgery Department The First Hospital of Jilin University Jilin ChinaThoracic Surgery Department The First Hospital of Jilin University Jilin ChinaCancer Center The First Hospital of Jilin University Jilin ChinaCancer Center The First Hospital of Jilin University Jilin ChinaMultiple primary lung cancers (MPLCs) refers to two or more primary malignant tumors that occur simultaneously or successively in the lung of the same patient. Distinguishing MPLCs from intrapulmonary metastases is important for treatment strategy and prognosis. MPLCs have been considered as having different origins and clonal evolutionary processes. Whole genome sequencing (WGS) and whole exome sequencing (WES) are regarded as an effective way to identify the relationship and differentiation among MPLC nodules. Here, we report the case of a 63‐year‐old MPLC male patient who smoked for 40 years. Two nodules were found on chest computed tomography (CT) scan, which were further confirmed by pathology to be lung adenocarcinoma (ADC) and lung squamous cell carcinoma (SCC), respectively. WES of the two different nodules was performed, and the results showed that there was a significant genetic difference between the two nodules. Further analysis of the tumor mutation burden (TMB) of the two tumor lesions showed that the TMB of the squamous cell carcinoma was higher than that of the adenocarcinoma, indicating that the squamous cell carcinoma had a higher mutation frequency. According to the pathology and WES sequencing results, MPLCs for this case were regarded as independent of each other, with different origins and clonal evolutionary processes. In this case report, we emphasize that WES should play an important role in determining the origin of MPLC clones, and also make some explorations for the further discovery of new potential driver genes and therapeutic targets.https://doi.org/10.1111/1759-7714.13540Multiple primary lung cancers (MPLCs)non‐small cell lung cancer (NSCLC)whole exome sequencing (WES)
collection DOAJ
language English
format Article
sources DOAJ
author Yuxi Bai
Yinghui Xu
Xu Wang
Yunpeng Liu
Chao Sun
Ye Guo
Yangyang Cai
Guoguang Shao
Zhiguang Yang
Shi Qiu
Kewei Ma
spellingShingle Yuxi Bai
Yinghui Xu
Xu Wang
Yunpeng Liu
Chao Sun
Ye Guo
Yangyang Cai
Guoguang Shao
Zhiguang Yang
Shi Qiu
Kewei Ma
Whole exome sequencing of lung adenocarcinoma and lung squamous cell carcinoma in one individual: A case report
Thoracic Cancer
Multiple primary lung cancers (MPLCs)
non‐small cell lung cancer (NSCLC)
whole exome sequencing (WES)
author_facet Yuxi Bai
Yinghui Xu
Xu Wang
Yunpeng Liu
Chao Sun
Ye Guo
Yangyang Cai
Guoguang Shao
Zhiguang Yang
Shi Qiu
Kewei Ma
author_sort Yuxi Bai
title Whole exome sequencing of lung adenocarcinoma and lung squamous cell carcinoma in one individual: A case report
title_short Whole exome sequencing of lung adenocarcinoma and lung squamous cell carcinoma in one individual: A case report
title_full Whole exome sequencing of lung adenocarcinoma and lung squamous cell carcinoma in one individual: A case report
title_fullStr Whole exome sequencing of lung adenocarcinoma and lung squamous cell carcinoma in one individual: A case report
title_full_unstemmed Whole exome sequencing of lung adenocarcinoma and lung squamous cell carcinoma in one individual: A case report
title_sort whole exome sequencing of lung adenocarcinoma and lung squamous cell carcinoma in one individual: a case report
publisher Wiley
series Thoracic Cancer
issn 1759-7706
1759-7714
publishDate 2020-08-01
description Multiple primary lung cancers (MPLCs) refers to two or more primary malignant tumors that occur simultaneously or successively in the lung of the same patient. Distinguishing MPLCs from intrapulmonary metastases is important for treatment strategy and prognosis. MPLCs have been considered as having different origins and clonal evolutionary processes. Whole genome sequencing (WGS) and whole exome sequencing (WES) are regarded as an effective way to identify the relationship and differentiation among MPLC nodules. Here, we report the case of a 63‐year‐old MPLC male patient who smoked for 40 years. Two nodules were found on chest computed tomography (CT) scan, which were further confirmed by pathology to be lung adenocarcinoma (ADC) and lung squamous cell carcinoma (SCC), respectively. WES of the two different nodules was performed, and the results showed that there was a significant genetic difference between the two nodules. Further analysis of the tumor mutation burden (TMB) of the two tumor lesions showed that the TMB of the squamous cell carcinoma was higher than that of the adenocarcinoma, indicating that the squamous cell carcinoma had a higher mutation frequency. According to the pathology and WES sequencing results, MPLCs for this case were regarded as independent of each other, with different origins and clonal evolutionary processes. In this case report, we emphasize that WES should play an important role in determining the origin of MPLC clones, and also make some explorations for the further discovery of new potential driver genes and therapeutic targets.
topic Multiple primary lung cancers (MPLCs)
non‐small cell lung cancer (NSCLC)
whole exome sequencing (WES)
url https://doi.org/10.1111/1759-7714.13540
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