The origin and migration of primordial germ cells in sturgeons.
Primordial germ cells (PGCs) arise elsewhere in the embryo and migrate into developing gonadal ridges during embryonic development. In several model animals, formation and migration patterns of PGCs have been studied, and it is known that these patterns vary. Sturgeons (genus Acipenser) have great p...
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doaj-44c8ccf73a3844fb9ea495464b0304392020-11-25T00:59:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8686110.1371/journal.pone.0086861The origin and migration of primordial germ cells in sturgeons.Taiju SaitoMartin PšeničkaRie GotoShinji AdachiKunio InoueKatsutoshi AraiEtsuro YamahaPrimordial germ cells (PGCs) arise elsewhere in the embryo and migrate into developing gonadal ridges during embryonic development. In several model animals, formation and migration patterns of PGCs have been studied, and it is known that these patterns vary. Sturgeons (genus Acipenser) have great potential for comparative and evolutionary studies of development. Sturgeons belong to the super class Actinoptergii, and their developmental pattern is similar to that of amphibians, although their phylogenetic position is an out-group to teleost fishes. Here, we reveal an injection technique for sturgeon eggs allowing visualization of germplasm and PGCs. Using this technique, we demonstrate that the PGCs are generated at the vegetal pole of the egg and they migrate on the yolky cell mass toward the gonadal ridge. We also provide evidence showing that PGCs are specified by inheritance of maternally supplied germplasm. Furthermore, we demonstrate that the migratory mechanism is well-conserved between sturgeon and other remotely related teleosts, such as goldfish, by a single PGCs transplantation (SPT) assay. The mode of PGCs specification in sturgeon is similar to that of anurans, but the migration pattern resembles that of teleosts.http://europepmc.org/articles/PMC3914811?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Taiju Saito Martin Pšenička Rie Goto Shinji Adachi Kunio Inoue Katsutoshi Arai Etsuro Yamaha |
spellingShingle |
Taiju Saito Martin Pšenička Rie Goto Shinji Adachi Kunio Inoue Katsutoshi Arai Etsuro Yamaha The origin and migration of primordial germ cells in sturgeons. PLoS ONE |
author_facet |
Taiju Saito Martin Pšenička Rie Goto Shinji Adachi Kunio Inoue Katsutoshi Arai Etsuro Yamaha |
author_sort |
Taiju Saito |
title |
The origin and migration of primordial germ cells in sturgeons. |
title_short |
The origin and migration of primordial germ cells in sturgeons. |
title_full |
The origin and migration of primordial germ cells in sturgeons. |
title_fullStr |
The origin and migration of primordial germ cells in sturgeons. |
title_full_unstemmed |
The origin and migration of primordial germ cells in sturgeons. |
title_sort |
origin and migration of primordial germ cells in sturgeons. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Primordial germ cells (PGCs) arise elsewhere in the embryo and migrate into developing gonadal ridges during embryonic development. In several model animals, formation and migration patterns of PGCs have been studied, and it is known that these patterns vary. Sturgeons (genus Acipenser) have great potential for comparative and evolutionary studies of development. Sturgeons belong to the super class Actinoptergii, and their developmental pattern is similar to that of amphibians, although their phylogenetic position is an out-group to teleost fishes. Here, we reveal an injection technique for sturgeon eggs allowing visualization of germplasm and PGCs. Using this technique, we demonstrate that the PGCs are generated at the vegetal pole of the egg and they migrate on the yolky cell mass toward the gonadal ridge. We also provide evidence showing that PGCs are specified by inheritance of maternally supplied germplasm. Furthermore, we demonstrate that the migratory mechanism is well-conserved between sturgeon and other remotely related teleosts, such as goldfish, by a single PGCs transplantation (SPT) assay. The mode of PGCs specification in sturgeon is similar to that of anurans, but the migration pattern resembles that of teleosts. |
url |
http://europepmc.org/articles/PMC3914811?pdf=render |
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