STAT3 induces colorectal carcinoma progression through a novel miR-572-MOAP-1 pathway
Nan Wang,1 Xianli He,1 Ru Zhou,2 Guozhan Jia,1 Qing Qiao1 1Department of General Surgery, Tangdu Hospital, Fourth Military Medical University, Xi’an, Shanxi, 710038, China; 2Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi’an, Shanxi, 710032, Chin...
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doaj-44eaf15b227c4c2d9e4e3b5da02a348a2020-11-24T21:22:19ZengDove Medical PressOncoTargets and Therapy1178-69302018-06-01Volume 113475348438851STAT3 induces colorectal carcinoma progression through a novel miR-572-MOAP-1 pathwayWang NHe XZhou RJia GQiao QNan Wang,1 Xianli He,1 Ru Zhou,2 Guozhan Jia,1 Qing Qiao1 1Department of General Surgery, Tangdu Hospital, Fourth Military Medical University, Xi’an, Shanxi, 710038, China; 2Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi’an, Shanxi, 710032, China Purpose: Colorectal carcinoma (CRC) is among the most common causes of death. Recent studies have shown that both STAT3 and miR-572 contribute to CRC progression. STAT3 plays an important role in miRNA expression. Moreover, MOAP-1, which is a pro-apoptotic protein that induces cell death or apoptosis, has a direct correlation with miRNA. Therefore, the current study is designed to explore whether miR-572 and STAT3 are involved in a common pathway and the role of MOAP-1 in this process. Patients and methods: The expressions of STAT3, miR-572, and MOAP-1 in human CRC tissues and multiple cell lines were estimated by qRT-PCR or Western blot. MTT, transwell migration, and invasion assays were used to assess cell growth, migration, and invasion, respectively. Dual-luciferase reporter assay was applied to examine the association between miR-572 and MOAP-1. Results: Elevated STAT3 levels were accompanied by increased miR-572 and decreased MOAP-1 levels in primary CRC specimens and cell lines. STAT3 promoted CRC cell growth, migration, and invasion via the upregulated expression of miR-572. Subsequently, miR-572 inhibited MOAP-1 protein expression through an interaction with its 3'UTR. Conclusion: Our study proposes a novel STAT3-miR-572-MOAP-1 pathway involved in the process of CRC progression, which might be a potential target for the development of new preventive and therapeutic approaches against human colorectal cancer. Keywords: colorectal neoplasm, STAT3, MOAP-1, miR-572, tumor progressionhttps://www.dovepress.com/stat3-induces-colorectal-carcinoma-progression-through-a-novel-mir-572-peer-reviewed-article-OTTcolorectal neoplasmSTAT3MOAP-1miR-572tumor progression |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wang N He X Zhou R Jia G Qiao Q |
spellingShingle |
Wang N He X Zhou R Jia G Qiao Q STAT3 induces colorectal carcinoma progression through a novel miR-572-MOAP-1 pathway OncoTargets and Therapy colorectal neoplasm STAT3 MOAP-1 miR-572 tumor progression |
author_facet |
Wang N He X Zhou R Jia G Qiao Q |
author_sort |
Wang N |
title |
STAT3 induces colorectal carcinoma progression through a novel miR-572-MOAP-1 pathway |
title_short |
STAT3 induces colorectal carcinoma progression through a novel miR-572-MOAP-1 pathway |
title_full |
STAT3 induces colorectal carcinoma progression through a novel miR-572-MOAP-1 pathway |
title_fullStr |
STAT3 induces colorectal carcinoma progression through a novel miR-572-MOAP-1 pathway |
title_full_unstemmed |
STAT3 induces colorectal carcinoma progression through a novel miR-572-MOAP-1 pathway |
title_sort |
stat3 induces colorectal carcinoma progression through a novel mir-572-moap-1 pathway |
publisher |
Dove Medical Press |
series |
OncoTargets and Therapy |
issn |
1178-6930 |
publishDate |
2018-06-01 |
description |
Nan Wang,1 Xianli He,1 Ru Zhou,2 Guozhan Jia,1 Qing Qiao1 1Department of General Surgery, Tangdu Hospital, Fourth Military Medical University, Xi’an, Shanxi, 710038, China; 2Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi’an, Shanxi, 710032, China Purpose: Colorectal carcinoma (CRC) is among the most common causes of death. Recent studies have shown that both STAT3 and miR-572 contribute to CRC progression. STAT3 plays an important role in miRNA expression. Moreover, MOAP-1, which is a pro-apoptotic protein that induces cell death or apoptosis, has a direct correlation with miRNA. Therefore, the current study is designed to explore whether miR-572 and STAT3 are involved in a common pathway and the role of MOAP-1 in this process. Patients and methods: The expressions of STAT3, miR-572, and MOAP-1 in human CRC tissues and multiple cell lines were estimated by qRT-PCR or Western blot. MTT, transwell migration, and invasion assays were used to assess cell growth, migration, and invasion, respectively. Dual-luciferase reporter assay was applied to examine the association between miR-572 and MOAP-1. Results: Elevated STAT3 levels were accompanied by increased miR-572 and decreased MOAP-1 levels in primary CRC specimens and cell lines. STAT3 promoted CRC cell growth, migration, and invasion via the upregulated expression of miR-572. Subsequently, miR-572 inhibited MOAP-1 protein expression through an interaction with its 3'UTR. Conclusion: Our study proposes a novel STAT3-miR-572-MOAP-1 pathway involved in the process of CRC progression, which might be a potential target for the development of new preventive and therapeutic approaches against human colorectal cancer. Keywords: colorectal neoplasm, STAT3, MOAP-1, miR-572, tumor progression |
topic |
colorectal neoplasm STAT3 MOAP-1 miR-572 tumor progression |
url |
https://www.dovepress.com/stat3-induces-colorectal-carcinoma-progression-through-a-novel-mir-572-peer-reviewed-article-OTT |
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